Open Access

Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection

  • Authors:
    • Ye Zhang
    • Shuo Feng
    • Wang Chen
    • Qing‑Chen Zhang
    • Si‑Feng Shi
    • Xiang‑Yang Chen
  • View Affiliations

  • Published online on: July 29, 2020     https://doi.org/10.3892/etm.2020.9082
  • Pages: 3104-3113
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

16S ribosomal RNA (rRNA) PCR has been reported to be an effective diagnostic means in patients with prosthetic joint infection (PJI). The aim of the present meta‑analysis is to establish the overall diagnostic accuracy of the measurement of 16S rRNA PCR for diagnosing PJI. PubMed, Web of Science, Cochrane Library, EMBASE and Wiley Online Library were searched for studies on 16S rRNA PCR in the diagnosis of PJI. The search incorporated all literature published up until December 2018 and the QUADAS‑2 checklist were used for quality assessment. The sensitivity, specificity and other measures of accuracy of 16S rRNA PCR in the diagnosis of PJI were pooled. Statistical analysis was performed by employing Meta‑Disc 1.4 and Stata 12.0 software. A total of 15 studies met the inclusion criteria. The summary estimates for 16S rRNA PCR in the diagnosis of PJI in these studies were pooled: Sensitivity, 0.70 (95% CI, 0.67‑0.73); specificity, 0.93 (95% CI, 0.91‑0.94); positive likelihood ratio, 10.93 (95% CI, 5.55‑21.51); negative likelihood ratio, 0.33 (95% CI, 0.28‑0.40); diagnostic odds ratio, 41.77 (95% CI, 19.90‑87.68); and the area under the curve, 0.89. Subgroup analysis showed that the use of sonicate fluid and periprosthetic tissue has higher sensitivity (0.76; 95% CI, 0.69‑0.82; and 0.73; 95% CI, 0.68‑0.78, respectively), specificity (0.93, 95% CI, 0.90‑0.96; and 0.95; 95% CI, 0.90‑0.98, respectively) and area under the curve (0.93 and 0.98, respectively). 16S rRNA PCR assay plays an important role in the diagnosis of PJI. The results of 16S rRNA PCR assays should be interpreted in parallel with clinical findings, the results of microbiological, and other laboratory tests.
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October-2020
Volume 20 Issue 4

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Y, Feng S, Chen W, Zhang QC, Shi SF and Chen XY: Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection. Exp Ther Med 20: 3104-3113, 2020
APA
Zhang, Y., Feng, S., Chen, W., Zhang, Q., Shi, S., & Chen, X. (2020). Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection. Experimental and Therapeutic Medicine, 20, 3104-3113. https://doi.org/10.3892/etm.2020.9082
MLA
Zhang, Y., Feng, S., Chen, W., Zhang, Q., Shi, S., Chen, X."Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection". Experimental and Therapeutic Medicine 20.4 (2020): 3104-3113.
Chicago
Zhang, Y., Feng, S., Chen, W., Zhang, Q., Shi, S., Chen, X."Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3104-3113. https://doi.org/10.3892/etm.2020.9082