Open Access

CLDN3 expression and function in pregnancy‑induced hypertension

  • Authors:
    • Aixin Zhao
    • Yunfang Qi
    • Kun Liu
  • View Affiliations

  • Published online on: July 31, 2020     https://doi.org/10.3892/etm.2020.9084
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

This aim of the present study was to investigate the expression and function of claudin 3 (CLDN3) in pregnancy‑induced hypertension. The mRNA expression levels of CLDN3 in the placental tissue and peripheral blood of patients with pregnancy‑induced hypertension were measured using reverse transcription‑quantitative PCR. Human trophoblast HTR8/SVneo cells overexpressing CLDN3 were generated using a lentiviral vector. Cell Counting kit‑8 (CCK‑8) assay, flow cytometry, Transwell chamber assays, confocal laser scanning microscopy and western blot analysis were performed to detect cell proliferation, invasion, migration and apoptosis, in addition to matrix metalloproteinase (MMP) expression and ERK1/2 phosphorylation. The mRNA expression levels of CLDN3 were significantly reduced in the placental tissues and peripheral blood samples of patients with pregnancy‑induced hypertension compared with healthy pregnant controls. CLDN3 overexpression significantly increased HTR8/SVneo cell proliferation, invasion and migration whilst reducing apoptosis. HTR8/SVneo cells overexpressing CLDN3 also exhibited increased myofiber levels, increased MMP‑2 and MMP‑9 expression and increased ERK1/2 signaling activity. CLDN3 downregulation may be associated with the pathogenesis of pregnancy‑induced hypertension. In conclusion, CLDN3 promotes the proliferative and invasive capabilities of human trophoblast cells, with the underlying mechanisms possibly involving upregulation of MMP expression via the ERK1/2 signaling pathway.

Related Articles

Journal Cover

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao A, Qi Y and Liu K: CLDN3 expression and function in pregnancy‑induced hypertension. Exp Ther Med 0: 0-0, 1899
APA
Zhao, A., Qi, Y., & Liu, K. (1899). CLDN3 expression and function in pregnancy‑induced hypertension. Experimental and Therapeutic Medicine, 0, 0-0. https://doi.org/10.3892/etm.2020.9084
MLA
Zhao, A., Qi, Y., Liu, K."CLDN3 expression and function in pregnancy‑induced hypertension". Experimental and Therapeutic Medicine 0.0 (1899): 0-0.
Chicago
Zhao, A., Qi, Y., Liu, K."CLDN3 expression and function in pregnancy‑induced hypertension". Experimental and Therapeutic Medicine 0, no. 0 (1899): 0-0. https://doi.org/10.3892/etm.2020.9084