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MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts

  • Authors:
    • Wei Liu
    • Chao Ma
    • Hao-Yu Li
    • Lan Chen
    • Shan-Si Yuan
    • Kai-Jun Li
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 38
    |
    Published online on: September 1, 2020
       https://doi.org/10.3892/etm.2020.9165
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Abstract

The present study aimed to investigate the effect of microRNA (miR)‑146a on the secretion of hyaluronic acid (HA) and collagen I in Graves' ophthalmopathy (GO) orbital fibroblasts, and identify potential novel targets for the clinical treatment of GO. Orbital fibroblasts were extracted from orbital connective tissue, and primary cells were identified via immunohistochemistry. The levels of HA and collagen I in orbital fibroblasts of non‑GO controls and patients with GO were examined via reverse transcription‑quantitative PCR (RT‑qPCR). miR‑146a was overexpressed or inhibited in primary orbital fibroblasts via lentiviral infection, and the levels of HA and collagen I following miR‑146a overexpression or inhibition were detected via ELISA and RT‑qPCR. The results indicated that the mRNA expression of HA and collagen I was higher in orbital fibroblasts from patients with GO compared with the non‑GO cohort. Overexpression of miR‑146a reduced, and inhibition of miR‑146a increased the production of HA and collagen I in GO orbital fibroblasts. In conclusion, it was demonstrated that miR‑146a downregulated the secretion of HA and collagen I in GO orbital fibroblasts in vitro, which may affect glycosaminoglycan aggregation and collagen deposition in GO.
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1 

Hiromatsu Y, Eguchi H, Tani J, Kasaoka M and Teshima Y: Graves' ophthalmopathy: Epidemiology and natural history. Intern Med. 53:353–360. 2014.PubMed/NCBI View Article : Google Scholar

2 

Wang L and Ma JM: Progression of the pathogenesis of thyroid associated ophthalmopathy. Zhonghua Yan Ke Za Zhi. 53:474–480. 2017.PubMed/NCBI View Article : Google Scholar : (In Chinese).

3 

Gu LQ, Jia HY, Zhao YJ, Liu N, Wang S, Cui B and Ning G: Association studies of interleukin-8 gene in Graves' disease and Graves' ophthalmopathy. Endocrine. 36:452–456. 2009.PubMed/NCBI View Article : Google Scholar

4 

Park M, Banga JP, Kim GJ, Kim M and Lew H: Human placenta-derived mesenchymal stem cells ameliorate orbital adipogenesis in female mice models of Graves' ophthalmopathy. Stem Cell Res Ther. 10(246)2019.PubMed/NCBI View Article : Google Scholar

5 

Bahn RS and Heufelder AE: Retroocular fibroblasts: Important effector cells in Graves' ophthalmopathy. Thyroid. 2:89–94. 1992.PubMed/NCBI View Article : Google Scholar

6 

Weetman AP, Cohen S, Gatter KC, Fells P and Shine B: Immunohistochemical analysis of the retrobulbar tissues in Graves' ophthalmopathy. Clin Exp Immunol. 75:222–227. 1989.PubMed/NCBI

7 

Khong JJ, McNab AA, Ebeling PR, Craig JE and Selva D: Pathogenesis of thyroid eye disease: Review and update on molecular mechanisms. Br J Ophthalmol. 100:142–150. 2016.PubMed/NCBI View Article : Google Scholar

8 

Kumar S, Coenen M, Iyer S and Bahn RS: Forkhead transcription factor FOXO1 is regulated by both a stimulatory thyrotropin receptor antibody and insulin-like growth factor-1 in orbital fibroblasts from patients with Graves' ophthalmopathy. Thyroid. 25:1145–1150. 2015.PubMed/NCBI View Article : Google Scholar

9 

Underhill CB: The interaction of hyaluronate with the cell surface: The hyaluronate receptor and the core protein. Ciba Found Symp. 143:87–99; discussion 100-106, 281-285. 1989.PubMed/NCBI View Article : Google Scholar

10 

van Steensel L, Paridaens D, Schrijver B, Dingjan GM, van Daele PL, van Hagen PM, van den Bosch WA, Drexhage HA, Hooijkaas H and Dik WA: Imatinib mesylate and AMN107 inhibit PDGF-signaling in orbital fibroblasts: A potential treatment for Graves' ophthalmopathy. Invest Ophthalmol Vis Sci. 50:3091–3098. 2009.PubMed/NCBI View Article : Google Scholar

11 

Jang SY, Park SJ, Chae MK, Lee JH, Lee EJ and Yoon JS: Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy. Br J Ophthalmol. 102:407–414. 2018.PubMed/NCBI View Article : Google Scholar

12 

Wang Y and Smith TJ: Current concepts in the molecular pathogenesis of thyroid-associated ophthalmopathy. Invest Ophthalmol Vis Sci. 55:1735–1748. 2014.PubMed/NCBI View Article : Google Scholar

13 

Li K, Du Y, Jiang BL and He JF: Increased microRNA-155 and decreased microRNA-146a may promote ocular inflammation and proliferation in Graves' ophthalmopathy. Med Sci Monit. 20:639–643. 2014.PubMed/NCBI View Article : Google Scholar

14 

Bhaskaran M and Mohan M: MicroRNAs: History, biogenesis, and their evolving role in animal development and disease. Vet Pathol. 51:759–774. 2014.PubMed/NCBI View Article : Google Scholar

15 

Bernardo BC, Ooi JYY, Lin RCY and McMullen JR: miRNA therapeutics: A new class of drugs with potential therapeutic applications in the heart. Future Med Chem. 7:1771–1792. 2015.PubMed/NCBI View Article : Google Scholar

16 

Wu F, Zikusoka M, Trindade A, Dassopoulos T, Harris ML, Bayless TM, Brant SR, Chakravarti S and Kwon JH: MicroRNAs are differentially expressed in ulcerative colitis and alter expression of macrophage inflammatory peptide-2α. Gastroenterology. 135:1624–1635.e24. 2008.PubMed/NCBI View Article : Google Scholar

17 

Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M and Asahara H: Expression of microRNA-146 in rheumatoid arthritis synovial tissue. Arthritis Rheum. 58:1284–1292. 2008.PubMed/NCBI View Article : Google Scholar

18 

Tang Y, Luo X, Cui H, Ni X, Yuan M, Guo Y, Huang X, Zhou H, de Vries N, Tak PP, et al: MicroRNA-146A contributes to abnormal activation of the type I interferon pathway in human lupus by targeting the key signaling proteins. Arthritis Rheum. 60:1065–1075. 2009.PubMed/NCBI View Article : Google Scholar

19 

Jang SY, Chae MK, Lee JH, Lee EJ and Yoon JS: Role of miR-146a in the regulation of inflammation in an in vitro model of Graves' orbitopathy. Invest Ophthalmol Vis Sci. 57:4027–4034. 2016.PubMed/NCBI View Article : Google Scholar

20 

Wang N, Chen FE and Long ZW: Mechanism of microrna-146a/notch2 signaling regulating il-6 in Graves ophthalmopathy. Cell Physiol Biochem. 41:1285–1297. 2017.PubMed/NCBI View Article : Google Scholar

21 

Li H, Ma C, Liu W, He J and Li K: Gypenosides protect orbital fibroblasts in Graves ophthalmopathy via anti-inflammation and anti-fibrosis effects. Invest Ophthalmol Vis Sci. 61(64)2020.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Blandford Z, Zhang D, Chundury RV and Perry JD: Dysthyroid optic neuropathy: Update on pathogenesis, diagnosis, and management. Expert Rev Ophthalmol. 12:111–121. 2017.PubMed/NCBI View Article : Google Scholar

24 

Yang IH, Rose GE, Ezra DG and Bailly M: Macrophages promote a profibrotic phenotype in orbital fibroblasts through increased hyaluronic acid production and cell contractility. Sci Rep. 9(9622)2019.PubMed/NCBI View Article : Google Scholar

25 

Iyer S and Bahn R: Immunopathogenesis of Graves' ophthalmopathy: The role of the TSH receptor. Best Pract Res Clin Endocrinol Metab. 26:281–289. 2012.PubMed/NCBI View Article : Google Scholar

26 

Longo CM and Higgins PJ: Molecular biomarkers of Graves' ophthalmopathy. Exp Mol Pathol. 106:1–6. 2019.PubMed/NCBI View Article : Google Scholar

27 

Jang SY, Chae MK, Lee JH, Lee EJ and Yoon JS: MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathy. PLoS One. 14(e0221077)2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Ma C, Li H, Chen L, Yuan S and Li K: MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts. Exp Ther Med 20: 38, 2020.
APA
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., & Li, K. (2020). MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts. Experimental and Therapeutic Medicine, 20, 38. https://doi.org/10.3892/etm.2020.9165
MLA
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., Li, K."MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts". Experimental and Therapeutic Medicine 20.5 (2020): 38.
Chicago
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., Li, K."MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts". Experimental and Therapeutic Medicine 20, no. 5 (2020): 38. https://doi.org/10.3892/etm.2020.9165
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Ma C, Li H, Chen L, Yuan S and Li K: MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts. Exp Ther Med 20: 38, 2020.
APA
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., & Li, K. (2020). MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts. Experimental and Therapeutic Medicine, 20, 38. https://doi.org/10.3892/etm.2020.9165
MLA
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., Li, K."MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts". Experimental and Therapeutic Medicine 20.5 (2020): 38.
Chicago
Liu, W., Ma, C., Li, H., Chen, L., Yuan, S., Li, K."MicroRNA-146a downregulates the production of hyaluronic acid and collagen I in Graves' ophthalmopathy orbital fibroblasts". Experimental and Therapeutic Medicine 20, no. 5 (2020): 38. https://doi.org/10.3892/etm.2020.9165
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