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Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury

  • Authors:
    • Xuewen Guo
    • Wenyang Li
    • Ran An
    • Mei Huang
    • Zugong Yu
  • View Affiliations / Copyright

    Affiliations: Department of Veterinary Preventive Medicine, Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China, Department of Veterinary Preventive Medicine, Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Article Number: 52
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    Published online on: September 4, 2020
       https://doi.org/10.3892/etm.2020.9180
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Abstract

Composite ammonium glycyrrhizin (CAG) has anti‑inflammatory activity. Lipopolysaccharide (LPS) and enrofloxacin (ENR) induce liver damage; however, the mechanism underlying LPS/ENR‑induced hepatic injury remains to be elucidated. In the present study, the mechanism of LPS/ENR‑induced liver injury was investigated in vitro and the protective effects of CAG were also evaluated. Primary chicken hepatocytes were isolated and a model of LPS/ENR‑induced hepatocyte injury was established. mRNA and protein expression levels were evaluated by reverse transcription‑quantitative polymerase chain reaction and western blot, respectively. LPS/ENR exposure significantly increased supernatant aspartate aminotransferase (AST) and alanine aminotransferase (ALT). In the LPS/ENR‑treated group, glutathione (GSH) and the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were significantly increased. Flow cytometry results revealed that the apoptotic rate significantly increased in the LPS/ENR‑treated group compared with the control, while treatment with CAG given 24 h prior to LPS/ENR caused a significant decrease in the apoptotic rate compared with the model group. Furthermore, CAG treatment reversed LPS/ENR‑associated alterations in the mRNA and protein expression of Caspase‑3, apoptosis regulator Bcl‑2 (Bcl‑2) and Bcl‑2 associated X‑protein. The mitochondrial membrane potential significantly decreased and the mitochondrial microstructure was notably altered following exposure to LPS/ENR compared with the control. In conclusion, these results suggested that LPS/ENR‑treated hepatocytes were damaged via apoptotic signaling pathways and CAG prevented LPS/ENR‑induced hepatocyte injury.
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Copy and paste a formatted citation
Spandidos Publications style
Guo X, Li W, An R, Huang M and Yu Z: Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury. Exp Ther Med 20: 52, 2020.
APA
Guo, X., Li, W., An, R., Huang, M., & Yu, Z. (2020). Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury. Experimental and Therapeutic Medicine, 20, 52. https://doi.org/10.3892/etm.2020.9180
MLA
Guo, X., Li, W., An, R., Huang, M., Yu, Z."Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury". Experimental and Therapeutic Medicine 20.5 (2020): 52.
Chicago
Guo, X., Li, W., An, R., Huang, M., Yu, Z."Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury". Experimental and Therapeutic Medicine 20, no. 5 (2020): 52. https://doi.org/10.3892/etm.2020.9180
Copy and paste a formatted citation
x
Spandidos Publications style
Guo X, Li W, An R, Huang M and Yu Z: Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury. Exp Ther Med 20: 52, 2020.
APA
Guo, X., Li, W., An, R., Huang, M., & Yu, Z. (2020). Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury. Experimental and Therapeutic Medicine, 20, 52. https://doi.org/10.3892/etm.2020.9180
MLA
Guo, X., Li, W., An, R., Huang, M., Yu, Z."Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury". Experimental and Therapeutic Medicine 20.5 (2020): 52.
Chicago
Guo, X., Li, W., An, R., Huang, M., Yu, Z."Composite ammonium glycyrrhizin has hepatoprotective effects in chicken hepatocytes with lipopolysaccharide/enrofloxacin‑induced injury". Experimental and Therapeutic Medicine 20, no. 5 (2020): 52. https://doi.org/10.3892/etm.2020.9180
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