Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2020 Volume 20 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2020 Volume 20 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Paclitaxel inhibits the migration of CD133+ U251 malignant glioma cells by reducing the expression of glycolytic enzymes

  • Authors:
    • Niya Long
    • Shuo Peng
    • Liangzhao Chu
    • Jun Jia
    • Minghao Dong
    • Jian Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Neurosurgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Long et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 72
    |
    Published online on: September 9, 2020
       https://doi.org/10.3892/etm.2020.9200
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Energy metabolic reprogramming (EMR) allows for the rearrangement of a series of metabolic genes and proteins when tumor cells adapt to their microenvironment. EMR is characterized by changes in the metabolic pattern and metabolic intermediates to meet the needs of tumor cells for their malignant proliferation and infiltrative growth. The present study investigated the role of low‑dose paclitaxel (PTX) in changing the expression levels of key genes and proteins during glycolysis in CD133+ U251 glioma cells and explored the relevant regulatory mechanisms of action at the molecular level. CD133 immunomagnetic beads were applied to malignant CD133+ U251 glioma cells, which were then divided into a negative control and an experimental group treated with 1, 2, 4 or 8 µM PTX for 72 h. Cell Counting Kit‑8 (CCK‑8) was used to measure U251 cell proliferation. RNA and protein were extracted from the malignant glioma cells in all groups to observe changes in the expression levels of key glycolytic enzymes, such as glucose transporter 1 (GLUT1), pyruvate kinase M (PKM) and lactate dehydrogenase A (LDHA), using reverse transcription‑quantitative PCR and western blot assays. Transwell migration assays were performed to quantify the effects of PTX solution on U251 cells. CD133+ U251 glioma cells were isolated successfully. CD1133+ cells had a higher rate of proliferation compared with CD1133‑ cells. In CD1133+ cells treated with PTX, a dose‑dependent reduction in the expression levels of the key glycolytic enzymes GLUT1, PKM and LDHA was observed at both the mRNA and protein levels. PTX solution also inhibited cell migration. Differences between the control and experimental groups were statistically significant (P<0.05). Since glycolysis plays an indispensable role in the proliferation and migration of stem cell‑like glioma cells, PTX may inhibit tumor cell growth by downregulating the gene and protein expression levels of glycolytic enzymes in CD133+ glioma cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Fan X, Li Y, Shan X, You G, Wu Z, Li Z, Qiao H and Jiang T: Seizures at presentation are correlated with better survival outcomes in adult diffuse glioma: A systematic review and meta-analysis. Seizure. 59:16–23. 2018.PubMed/NCBI View Article : Google Scholar

2 

Sun X, Chen Y, Zhao H, Qiao G, Liu M, Zhang C, Cui D and Ma L: Dual-modified cationic liposomes loaded with paclitaxel and survivin siRNA for targeted imaging and therapy of cancer stem cells in brain glioma. Drug Deliv. 25:1718–1727. 2018.PubMed/NCBI View Article : Google Scholar

3 

Bastiancich C, Bianco J, Vanvarenberg K, Ucakar B, Joudiou N, Gallez B, Bastiat G, Lagarce F, Préat V and Danhier F: Injectable nanomedicine hydrogel for local chemotherapy of glioblastoma after surgical resection. J Control Release. 264:45–54. 2017.PubMed/NCBI View Article : Google Scholar

4 

Jiapaer S, Furuta T, Tanaka S, Kitabayashi T and Nakada M: Potential Strategies Overcoming the Temozolomide Resistance for Glioblastoma. Neurol Med Chir (Tokyo). 58:405–421. 2018.PubMed/NCBI View Article : Google Scholar

5 

Sakamoto D, Takagi T, Fujita M, Omura S, Yoshida Y, Iida T and Yoshimura S: Basic Gene Expression Characteristics of Glioma Stem Cells and Human Glioblastoma. Anticancer Res. 39:597–607. 2019.PubMed/NCBI View Article : Google Scholar

6 

Liebelt BD, Shingu T, Zhou X, Ren J, Shin SA and Hu J: Glioma stem cells: Signaling, microenvironment, and therapy. Stem Cells Int. 2016(7849890)2016.PubMed/NCBI View Article : Google Scholar

7 

Seyfried TN, Flores R, Poff AM, D'Agostino DP and Mukherjee P: Metabolic therapy: A new paradigm for managing malignant brain cancer. Cancer Lett. 356A:289–300. 2015.PubMed/NCBI View Article : Google Scholar

8 

Nakano I: Stem cell signature in glioblastoma: Therapeutic development for a moving target. J Neurosurg. 122:324–330. 2015.PubMed/NCBI View Article : Google Scholar

9 

Codrici E, Enciu AM, Popescu ID, Mihai S and Tanase C: Glioma Stem Cells and Their Microenvironments: Providers of Challenging Therapeutic Targets. Stem Cells Int. 2016(5728438)2016.PubMed/NCBI View Article : Google Scholar

10 

Shokoohinia Y, Jafari F, Mohammadi Z, Bazvandi L, Hosseinzadeh L, Chow N, Bhattacharyya P, Farzaei MH, Farooqi AA, Nabavi SM, et al: Potential Anticancer Properties of Osthol: A Comprehensive Mechanistic Review. Nutrients. 10:36–51. 2018.PubMed/NCBI View Article : Google Scholar

11 

Gao C, He XF, Xu QR, Xu YJ and Shen J: Sevoflurane downregulates insulin-like growth factor-1 to inhibit cell proliferation, invasion and trigger apoptosis in glioma through the PI3K/AKT signaling pathway. Anticancer Drugs. 30(e0744)2019.PubMed/NCBI View Article : Google Scholar

12 

Eissa IR, Bustos-Villalobos I, Ichinose T, Matsumura S, Naoe Y, Miyajima N, Morimoto D, Mukoyama N, Zhiwen W, Tanaka M, et al: The Current Status and Future Prospects of Oncolytic Viruses in Clinical Trials against Melanoma, Glioma, Pancreatic, and Breast Cancers. Cancers (Basel). 10(E356)2018.PubMed/NCBI View Article : Google Scholar

13 

Shen X, Liu X, Wan S, Fan X, He H, Wei R, Pu W, Peng Y and Wang C: Discovery of coumarin as microtubule affinity-regulating kinase 4 inhibitor that sensitize hepatocellular carcinoma to paclitaxel. Front Chem. 7(366)2019.PubMed/NCBI View Article : Google Scholar

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

15 

Barbato L, Bocchetti M, Di Biase A and Regad T: Cancer stem cells and targeting strategies. Cells. 8:1–19. 2019.PubMed/NCBI View Article : Google Scholar

16 

Seano G: Targeting the perivascular niche in brain tumors. Curr Opin Oncol. 30:54–60. 2018.PubMed/NCBI View Article : Google Scholar

17 

Katayama R, Sakashita T, Yanagitani N, Ninomiya H, Horiike A, Friboulet L, Gainor JF, Motoi N, Dobashi A, Sakata S, et al: P-glycoprotein Mediates Ceritinib Resistance in Anaplastic Lymphoma Kinase-rearranged Non-small Cell Lung Cancer. EBio Medicine. 12:54–66. 2015.PubMed/NCBI View Article : Google Scholar

18 

Hossain M, Banik NL and Ray SK: Synergistic anti-cancer mechanisms of curcumin and paclitaxel for growth inhibition of human brain tumor stem cells and LN18 and U138MG cells. Neurochem Int. 61:1102–1113. 2012.PubMed/NCBI View Article : Google Scholar

19 

Jiang X, Xin H, Ren Q, Gu J, Zhu L, Du F, Feng C, Xie Y, Sha X and Fang X: Nanoparticles of 2-deoxy-D-glucose functionalized poly(ethylene glycol)-co-poly(trimethylene carbonate) for dual-targeted drug delivery in glioma treatment. Biomaterials. 35:518–529. 2014.PubMed/NCBI View Article : Google Scholar

20 

Ancey PB, Contat C and Meylan E: Glucose transporters in cancer - from tumor cells to the tumor microenvironment. FEBS J. 285:2926–2943. 2018.PubMed/NCBI View Article : Google Scholar

21 

Dacevic MP, Tasic JS, Pejanovic VM, Segal MB, Ugliesic-Kilibarda DD, Isakovic AJ, Begley DJ, Rakic LM and Redzic ZB: The linkage of glucose to tiazofurin decreases in vitro uptake into rat glioma C6 cells. J Drug Target. 10:633–636. 2002.PubMed/NCBI View Article : Google Scholar

22 

Oh S, Kim H, Nam K and Shin I: Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells. BMB Rep. 50:132–137. 2017.PubMed/NCBI View Article : Google Scholar

23 

Madunić IV, Madunić J, Breljak D, Karaica D and Sabolić I: Sodium-glucose cotransporters: New targets of cancer therapy? Arh Hig Rada Toksikol. 69:278–285. 2018.PubMed/NCBI View Article : Google Scholar

24 

Jin X, Su H, Ding G, Sun Z and Li Z: Exposure to ambient fine particles causes abnormal energy metabolism and ATP decrease in lung tissues. Chemosphere. 224:29–38. 2019.PubMed/NCBI View Article : Google Scholar

25 

Ma Z, Cui X, Lu L, Chen G, Yang Y, Hu Y, Lu Y, Cao Z, Wang Y and Wang X: Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis. Stem Cell Res Ther. 10(60)2019.PubMed/NCBI View Article : Google Scholar

26 

Tech K, Tikunov AP, Farooq H, Morrissy AS, Meidinger J, Fish T, Green SC, Liu H, Li Y, Mungall AJ, et al: Pyruvate kinase inhibits proliferation during postnatal cerebellar neurogenesis and suppresses medulloblastoma formation. Cancer Res. 77:3217–3230. 2017.PubMed/NCBI View Article : Google Scholar

27 

Miao P, Sheng S, Sun X, Liu J and Huang G: Lactate dehydrogenase A in cancer: A promising target for diagnosis and therapy. IUBMB Life. 65:904–910. 2013.PubMed/NCBI View Article : Google Scholar

28 

Xintaropoulou C, Ward C, Wise A, Queckborner S, Turnbull A, Michie CO, Williams ARW, Rye T, Gourley C and Langdon SP: Expression of glycolytic enzymes in ovarian cancers and evaluation of the glycolytic pathway as a strategy for ovarian cancer treatment. BMC Cancer. 18:636–651. 2018.PubMed/NCBI View Article : Google Scholar

29 

Li S, Gao J, Zhuang X, Zhao C, Hou X, Xing X, Chen C, Liu Q, Liu S and Luo Y: Cyclin G2 Inhibits the Warburg Effect and Tumour Progression by Suppressing LDHA Phosphorylation in Glioma. Int J Biol Sci. 15:544–555. 2019.PubMed/NCBI View Article : Google Scholar

30 

Di H, Zhang X, Guo Y, Shi Y, Fang C, Yuan Y, Wang J, Shang C, Guo W and Li C: Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells. Oncol Lett. 15:5131–5136. 2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Long N, Peng S, Chu L, Jia J, Dong M and Liu J: Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes. Exp Ther Med 20: 72, 2020.
APA
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., & Liu, J. (2020). Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes. Experimental and Therapeutic Medicine, 20, 72. https://doi.org/10.3892/etm.2020.9200
MLA
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., Liu, J."Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes". Experimental and Therapeutic Medicine 20.5 (2020): 72.
Chicago
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., Liu, J."Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes". Experimental and Therapeutic Medicine 20, no. 5 (2020): 72. https://doi.org/10.3892/etm.2020.9200
Copy and paste a formatted citation
x
Spandidos Publications style
Long N, Peng S, Chu L, Jia J, Dong M and Liu J: Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes. Exp Ther Med 20: 72, 2020.
APA
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., & Liu, J. (2020). Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes. Experimental and Therapeutic Medicine, 20, 72. https://doi.org/10.3892/etm.2020.9200
MLA
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., Liu, J."Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes". Experimental and Therapeutic Medicine 20.5 (2020): 72.
Chicago
Long, N., Peng, S., Chu, L., Jia, J., Dong, M., Liu, J."Paclitaxel inhibits the migration of CD133<sup>+</sup> U251 malignant glioma cells by reducing the expression of glycolytic enzymes". Experimental and Therapeutic Medicine 20, no. 5 (2020): 72. https://doi.org/10.3892/etm.2020.9200
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team