Open Access

Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells

  • Authors:
    • Chengchun Shen
    • Yan Li
    • Yunlin Chen
    • Lei Huang
    • Feng Zhang
    • Wei Wu
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  • Published online on: September 18, 2020     https://doi.org/10.3892/etm.2020.9227
  • Article Number: 106
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The mechanisms of intervertebral disc degeneration (IDD) involve numerous factors, including loss of the extracellular matrix (ECM) and vascular ingrowth. Melatonin has been reported to protect intervertebral discs (IVDs) from degeneration and to exert a potential anti‑angiogenic effect. The aim of the present study was to investigate the anti‑angiogenic and anabolic effects of melatonin in IVDs. Human nucleus pulposus (NP) and degenerative nucleus pulposus (DNP) cells were isolated and treated with melatonin. The results indicated that melatonin promoted ECM synthesis and NP cell proliferation. In addition, an NP/DNP and human umbilical vein endothelial cell (HUVEC) co‑culture model was used to investigate the anti‑angiogenesis effect of melatonin. Melatonin was indicated to suppress tube formation and migration of HUVECs in culture with NP cell‑conditioned medium, as well as in an NP cell co‑culture model. Fluorescence‑labeled vascular endothelial growth factor (VEGF) was used to study the binding between VEGF and its receptor. The results of the present study indicated that melatonin exerts an angiogenic effect via inhibition of the binding of VEGF to its receptor in HUVECs. Taken together, these results suggest that melatonin is a potential agent to prevent IDD.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shen C, Li Y, Chen Y, Huang L, Zhang F and Wu W: Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells. Exp Ther Med 20: 106, 2020
APA
Shen, C., Li, Y., Chen, Y., Huang, L., Zhang, F., & Wu, W. (2020). Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells. Experimental and Therapeutic Medicine, 20, 106. https://doi.org/10.3892/etm.2020.9227
MLA
Shen, C., Li, Y., Chen, Y., Huang, L., Zhang, F., Wu, W."Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells". Experimental and Therapeutic Medicine 20.5 (2020): 106.
Chicago
Shen, C., Li, Y., Chen, Y., Huang, L., Zhang, F., Wu, W."Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells". Experimental and Therapeutic Medicine 20, no. 5 (2020): 106. https://doi.org/10.3892/etm.2020.9227