Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2020 Volume 20 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2020 Volume 20 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture

  • Authors:
    • Haoyuan Gao
    • Xuyou Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Fourth Hospital of Jinan, Jinan, Shandong 250021, P.R. China, Department of Laboratory Medicine, Wulian People's Hospital, Shandong 262300, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 105
    |
    Published online on: September 18, 2020
       https://doi.org/10.3892/etm.2020.9235
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the changes in miRNA‑142 and bone morphogenetic protein‑2 (BMP‑2) expression before and after hip replacement surgery, and to determine their association with receptor activator of NF‑κB ligand (RANKL) and osteoprotegerin (OPG). For this purpose, 142 cases of hip arthroplasty in patients with femoral neck fracture were selected as the research group, and 50 cases of healthy individuals who underwent a physical examination during the same time period were selected the control group. Serum miR‑142 and BMP‑2 levels were measured by RT‑qPCR before and after surgery in the research group and the control group. Serum RANKL and OPG levels were detected before and after surgery by enzyme‑linked immunosorbent assay (ELISA). The levels of serum miR‑142 and BMP‑2 in the research group were significantly lower than those in the control group. At 1 month after surgery, the levels of serum miR‑142 and BMP‑2 in the research group were significantly higher than those before surgery, and at 6 months after surgery, and they were higher than those in the control group. Pearson's correlation analysis revealed that the serum levels of miR‑142 positively correlated with the BMP‑2 levels before surgery, at 1 month after surgery, and at 6 months after surgery in the research group. The results of ROC curve analysis revealed that the AUC values of serum miR‑142 and BMP‑2 were 0.911 and 0.861, respectively. At 1 month after surgery, the levels of serum miR‑142 and BMP‑2 in group A (patients with a good or excellent recovery) were significantly higher than those in group B (patients with a fair or poor recovery). The levels of serum RANKL and OPG in the research group significantly increased at 1 month after surgery. The serum levels of miR‑142 and BMP‑2 positively correlated with those of RANKL and OPG before surgery and at 1 month after surgery. On the whole, the findings of the present study indicate that pre‑operative serum miR‑142 and BMP‑2 levels are valuable for evaluating the post‑operative recovery of patients with femoral neck fracture undergoing hip replacement surgery. In addition, in the present study, at 1 month after surgery, the levels of both miR‑142 and BMP‑2 were related to the recovery of the patients, and positively correlated with the RANKL and OPG levels.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Yu L, Wang Y and Chen J: Total hip arthroplasty versus hemiarthroplasty for displaced femoral neck fractures: Meta-analysis of randomized trials. Clin Orthop Relat Res. 470:2235–2243. 2012.PubMed/NCBI View Article : Google Scholar

2 

Lowe JA, Crist BD, Bhandari M and Ferguson TA: Optimal treatment of femoral neck fractures according to patient's physiologic age: An evidence-based review. Orthop Clin North Am. 41:157–166. 2010.PubMed/NCBI View Article : Google Scholar

3 

Miyamoto RG, Kaplan KM, Levine BR, Egol KA and Zuckerman JD: Surgical management of hip fractures: An evidence-based review of the literature. II: Intertrochanteric fractures. J Am Acad Orthop Surg. 16:596–607. 2008.PubMed/NCBI View Article : Google Scholar

4 

Alnahhal A, Aslam-Pervez N and Sheikh HQ: Templating Hip arthroplasty. Open Access Maced J Med Sci. 7(672)2019.PubMed/NCBI View Article : Google Scholar

5 

Nemes S, Lind D, Cnudde P, Bülow E, Rolfson O and Rogmark C: Relative survival following hemi-and total hip arthroplasty for hip fractures in Sweden. BMC Musculoskelet Disord. 19(407)2018.PubMed/NCBI View Article : Google Scholar

6 

Maratt JD, Gagnier JJ, Butler PD, Hallstrom BR, Urquhart AG and Roberts KC: No difference in dislocation seen in anterior vs posterior approach total hip arthroplasty. J Arthroplasty. 31:127–130. 2016.PubMed/NCBI View Article : Google Scholar

7 

Li D, Liu J, Guo B, Liang C, Dang L, Lu C, He X, Cheung HY, Xu L, Lu C, et al: Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation. Nat Commun. 7(10872)2016.PubMed/NCBI View Article : Google Scholar

8 

Lian JB, Stein GS, van Wijnen AJ, Stein JL, Hassan MQ, Gaur T and Zhang Y: MicroRNA control of bone formation and homeostasis. Nat Rev Endocrinol. 8(212)2012.PubMed/NCBI View Article : Google Scholar

9 

Xu R, Bi C, Song J, Wang L, Ge C, Liu X and Zhang M: Upregulation of miR-142-5p in atherosclerotic plaques and regulation of oxidized low-density lipoprotein-induced apoptosis in macrophages. Mol Med Rep. 11:3229–3234. 2015.PubMed/NCBI View Article : Google Scholar

10 

Schaefer JS, Montufar-Solis D, Vigneswaran N and Klein JR: Selective upregulation of microRNA expression in peripheral blood leukocytes in IL-10-/- mice precedes expression in the colon. J Immunol. 187:5834–5841. 2011.PubMed/NCBI View Article : Google Scholar

11 

Zhang X, Yan Z, Zhang J, Gong L, Li W, Cui J, Liu Y, Gao Z, Li J, Shen L and Lu Y: Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Ann Oncol. 22:2257–2266. 2011.PubMed/NCBI View Article : Google Scholar

12 

Wang YK, Yu X, Cohen DM, Wozniak MA, Yang MT, Gao L, Eyckmans J and Chen CS: Bone morphogenetic protein-2-induced signaling and osteogenesis is regulated by cell shape, RhoA/ROCK, and cytoskeletal tension. Stem Cells Dev. 21:1176–1186. 2011.PubMed/NCBI View Article : Google Scholar

13 

Chen J, Li K, Pang Q, Yang C, Zhang H, Wu F, Cao H, Liu H, Wan Y, Xia W, et al: Identification of suitable reference gene and biomarkers of serum miRNAs for osteoporosis. Sci Rep. 6(36347)2016.PubMed/NCBI View Article : Google Scholar

14 

Mi M, Jin H, Wang B, Yukata K, Sheu TJ, Ke QH, Tong P, Im HJ, Xiao G and Chen D: Chondrocyte BMP2 signaling plays an essential role in bone fracture healing. Gene. 512:211–218. 2013.PubMed/NCBI View Article : Google Scholar

15 

Krege JH, Lane NE, Harris JM and Miller PD: PINP as a biological response marker during teriparatide treatment for osteoporosis. Osteoporos Int. 25:2159–2171. 2014.PubMed/NCBI View Article : Google Scholar

16 

Martin TJ and Sims NA: RANKL/OPG; Critical role in bone physiology. Rev Endocr Metab Disord. 16:131–139. 2015.PubMed/NCBI View Article : Google Scholar

17 

Cassuto J, Folestad A, Göthlin J, Malchau H and Kärrholm J: The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients. Bone. 107:66–77. 2018.PubMed/NCBI View Article : Google Scholar

18 

Davenport DL, Bowe EA, Henderson WG, Khuri SF and Mentzer RM Jr: National surgical quality improvement program (NSQIP) risk factors can be used to validate American Society of Anesthesiologists Physical Status Classification (ASA PS) levels. Ann Surg. 243:636–644. 2006.PubMed/NCBI View Article : Google Scholar

19 

Wright NC, Saag KG, Dawson-Hughes B, Khosla S and Siris ES: The impact of the new National Bone Health Alliance (NBHA) diagnostic criteria on the prevalence of osteoporosis in the USA. Osteoporos Int. 28:1225–1232. 2017.PubMed/NCBI View Article : Google Scholar

20 

Sprague S, Bhandari M, Heetveld MJ, Liew S, Scott T, Bzovsky S, Heels-Ansdell D, Zhou Q, Swiontkowski M and Schemitsch EH: FAITH Investigators: Factors associated with health-related quality of life, hip function, and health utility after operative management of femoral neck fractures. Bone Joint J. 100-B:361–369. 2018.PubMed/NCBI View Article : Google Scholar

21 

Singh JA, Schleck C, Harmsen S and Lewallen D: Clinically important improvement thresholds for Harris Hip Score and its ability to predict revision risk after primary total hip arthroplasty. BMC Musculoskelet Disord. 17(256)2016.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Abdel MP, Watts CD, Houdek MT, Lewallen DG and Berry DJ: Epidemiology of periprosthetic fracture of the femur in 32 644 primary total hip arthroplasties: A 40-year experience. Bone Joint J. 98:461–467. 2016.PubMed/NCBI View Article : Google Scholar

24 

Mahmood SS, Mukka SS, Crnalic S, Wretenberg P and Sayed-Noor AS: Association between changes in global femoral offset after total hip arthroplasty and function, quality of life, and abductor muscle strength: A prospective cohort study of 222 patients. Acta Orthop. 87:36–41. 2016.PubMed/NCBI View Article : Google Scholar

25 

Angerame MR, Fehring TK, Masonis JL, Mason JB, Odum SM and Springer BD: Early failure of primary total hip arthroplasty: Is surgical approach a risk factor? J Arthroplasty. 33:1780–1785. 2018.PubMed/NCBI View Article : Google Scholar

26 

Lee WY, Li N, Lin S, Wang B, Lan HY and Li G: miRNA-29b improves bone healing in mouse fracture model. Mol Cell Endocrinol. 430:97–107. 2016.PubMed/NCBI View Article : Google Scholar

27 

Waki T, Lee SY, Niikura T, Iwakura T, Dogaki Y, Okumachi E, Oe K, Kuroda R and Kurosaka M: Profiling microRNA expression during fracture healing. BMC Musculoskelet Disord. 17(83)2016.PubMed/NCBI View Article : Google Scholar

28 

Liu X and Xu X: MicroRNA-137 dysregulation predisposes to osteoporotic fracture by impeding ALP activity and expression via suppression of leucine-rich repeat-containing G-protein-coupled receptor 4 expression. Int J Mol Med. 42:1026–1033. 2018.PubMed/NCBI View Article : Google Scholar

29 

Yao CJ, Lv Y, Zhang CJ, Jin JX, Xu LH, Jiang J, Geng B, Li H, Xia YY and Wu M: MicroRNA-185 inhibits the growth and proliferation of osteoblasts in fracture healing by targeting PTH gene through down-regulating Wnt/β-catenin axis: In an animal experiment. Biochem Biophys Res Commun. 501:55–63. 2018.PubMed/NCBI View Article : Google Scholar

30 

Rogers MB, Shah TA and Shaikh NN: Turning bone morphogenetic protein 2 (BMP2) on and off in mesenchymal cells. J Cell Biochem. 116:2127–2138. 2015.PubMed/NCBI View Article : Google Scholar

31 

Zhao R, Zhu Y and Sun B: Exploration of the Effect of mmu-miR-142-5p on Osteoblast and the Mechanism. Cell Biochem Biophys. 71:255–260. 2015.PubMed/NCBI View Article : Google Scholar

32 

Tsuji K, Bandyopadhyay A, Harfe BD, Cox K, Kakar S, Gerstenfeld L, Einhorn T, Tabin CJ and Rosen V: BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. Nat Genet. 38(1424)2006.PubMed/NCBI View Article : Google Scholar

33 

Singh JA, Schelck C, Harmsen S and Lewallen D: AB0757 Clinically important improvement thresholds for Harris hip score and its ability to predict revision risk after primary total hip arthroplasty. BMC Musculoskelet Disord. 17(256)2016.PubMed/NCBI View Article : Google Scholar

34 

Higgins BT, Barlow DR, Heagerty NE and Lin TJ: Anterior vs. posterior approach for total hip arthroplasty, a systematic review and meta-analysis. J Arthroplasty. 30:419–434. 2015.PubMed/NCBI View Article : Google Scholar

35 

de Amorim FP, Ornelas SS, Diniz SF, Batista AC and da Silva TA: Imbalance of RANK, RANKL and OPG expression during tibial fracture repair in diabetic rats. J Mol Histol. 39(401)2008.PubMed/NCBI View Article : Google Scholar

36 

Wang XF, Zhang YK, Yu ZS and Zhou JL: The role of the serum RANKL/OPG ratio in the healing of intertrochanteric fractures in elderly patients. Mol Med Rep. 7:1169–1172. 2013.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gao H and Wang X: Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture. Exp Ther Med 20: 105, 2020.
APA
Gao, H., & Wang, X. (2020). Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture. Experimental and Therapeutic Medicine, 20, 105. https://doi.org/10.3892/etm.2020.9235
MLA
Gao, H., Wang, X."Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture". Experimental and Therapeutic Medicine 20.5 (2020): 105.
Chicago
Gao, H., Wang, X."Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture". Experimental and Therapeutic Medicine 20, no. 5 (2020): 105. https://doi.org/10.3892/etm.2020.9235
Copy and paste a formatted citation
x
Spandidos Publications style
Gao H and Wang X: Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture. Exp Ther Med 20: 105, 2020.
APA
Gao, H., & Wang, X. (2020). Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture. Experimental and Therapeutic Medicine, 20, 105. https://doi.org/10.3892/etm.2020.9235
MLA
Gao, H., Wang, X."Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture". Experimental and Therapeutic Medicine 20.5 (2020): 105.
Chicago
Gao, H., Wang, X."Serum miRNA‑142 and BMP‑2 are markers of recovery following hip replacement surgery for femoral neck fracture". Experimental and Therapeutic Medicine 20, no. 5 (2020): 105. https://doi.org/10.3892/etm.2020.9235
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team