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Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model

  • Authors:
    • Yining Xiao
    • Mingyue Fan
    • Wei Jin
    • William A. Li
    • Yanqiu Jia
    • Yanhong Dong
    • Xin Jiang
    • Jing Xu
    • Nan Meng
    • Peiyuan Lv
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China, Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 109
    |
    Published online on: September 18, 2020
       https://doi.org/10.3892/etm.2020.9237
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Abstract

Lithium has been previously demonstrated to alleviate cognitive impairment caused by neurodegenerative diseases and acute brain injuries; however, the specific mechanism remains elusive. In the present study, the C57BL/6 mouse model of spatial cognitive impairment induced by repeated cerebral ischemia‑
reperfusion was established. Morris water maze test was performed to evaluate the levels of spatial cognitive impairment. Nissl staining was used to observe any morphological alterations, whilst western blotting was performed to measure the expression levels of microtubule‑associated protein light chain 3 (LC3) and Beclin1 in addition to mTOR phosphorylation. LiCl was found to significantly improve spatial learning and memory impairments according to data from the Morris water maze test. Nissl staining indicated that LiCl inhibited neuronal damage in the CA1 region of the hippocampus. Additionally, LiCl increased mTOR phosphorylation, reduced beclin1 expression and reduced the LC3 II/I expression ratio. Taken together, these findings suggest that LiCl may alleviate the spatial cognitive impairment induced by repeated cerebral ischemia‑reperfusion. This observation may be attributed to the inhibition of excessive autophagy by LiCl through mTOR signaling activation.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao Y, Fan M, Jin W, Li WA, Jia Y, Dong Y, Jiang X, Xu J, Meng N, Lv P, Lv P, et al: Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model. Exp Ther Med 20: 109, 2020.
APA
Xiao, Y., Fan, M., Jin, W., Li, W.A., Jia, Y., Dong, Y. ... Lv, P. (2020). Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model. Experimental and Therapeutic Medicine, 20, 109. https://doi.org/10.3892/etm.2020.9237
MLA
Xiao, Y., Fan, M., Jin, W., Li, W. A., Jia, Y., Dong, Y., Jiang, X., Xu, J., Meng, N., Lv, P."Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model". Experimental and Therapeutic Medicine 20.5 (2020): 109.
Chicago
Xiao, Y., Fan, M., Jin, W., Li, W. A., Jia, Y., Dong, Y., Jiang, X., Xu, J., Meng, N., Lv, P."Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model". Experimental and Therapeutic Medicine 20, no. 5 (2020): 109. https://doi.org/10.3892/etm.2020.9237
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao Y, Fan M, Jin W, Li WA, Jia Y, Dong Y, Jiang X, Xu J, Meng N, Lv P, Lv P, et al: Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model. Exp Ther Med 20: 109, 2020.
APA
Xiao, Y., Fan, M., Jin, W., Li, W.A., Jia, Y., Dong, Y. ... Lv, P. (2020). Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model. Experimental and Therapeutic Medicine, 20, 109. https://doi.org/10.3892/etm.2020.9237
MLA
Xiao, Y., Fan, M., Jin, W., Li, W. A., Jia, Y., Dong, Y., Jiang, X., Xu, J., Meng, N., Lv, P."Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model". Experimental and Therapeutic Medicine 20.5 (2020): 109.
Chicago
Xiao, Y., Fan, M., Jin, W., Li, W. A., Jia, Y., Dong, Y., Jiang, X., Xu, J., Meng, N., Lv, P."Lithium chloride ameliorated spatial cognitive impairment through activating mTOR phosphorylation and inhibiting excessive autophagy in the repeated cerebral ischemia‑reperfusion mouse model". Experimental and Therapeutic Medicine 20, no. 5 (2020): 109. https://doi.org/10.3892/etm.2020.9237
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