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Article

The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring

  • Authors:
    • Shi Yan
    • Fengjuan Wang
    • Qingyun Shi
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, P.R. China, Department of Obstetrics and Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China
  • Article Number: 135
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    Published online on: October 2, 2020
       https://doi.org/10.3892/etm.2020.9264
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Abstract

A maternal high‑fat diet (HFD) has been shown to exert deleterious effects on fetal programming by impairing embryo growth, and exerts a long‑term effect on the health of offspring. The present study aimed to evaluate the effect of a maternal HFD on the ovaries of offspring from the perspective of oxidative stress. Female C57BL/6J mice were randomly assigned to four groups fed various HFDs during the preconception (4 weeks) and gestation‑lactation periods. Offspring were fed a normal diet after weaning, and serum and ovaries were collected at 10 weeks of age. The developmental status of follicles was observed using hematoxylin and eosin staining. The serum oxidative stress levels and insulin resistance were detected using ELISA test kits. The expression of phosphorylated form of H2AX histone variant (γH2AX), forkhead box protein O3a (FOXO3a), Bcl‑2‑like protein 11 (Bim)and insulin‑like growth factor (IGF)‑1 in ovarian tissue was analyzed using reverse transcription‑quantitative PCR and western blot analyses to further explore the pathogenic mechanism. Prenatal exposure to a maternal HFD resulted in a reduced number of primordial and preantral follicles in the ovaries of offspring. Exposure to an HFD in the preconception period exerted a greater effect on the offspring compared with exposure to an HFD in the gestation‑lactation period. A reduction in fat intake during the preconception, gestation and lactation periods significantly reduced the associated adverse outcomes. The expression of genes (FOXO3a, IGF‑1, γH2AX and Bim) involved in oxidative stress showed a decreasing trend (high‑fat/high‑fat>high‑fat/control>control/high‑fat >control/control) in the ovaries of offspring. Overall, HFD exposure during the preconception period exerted a greater effect on offspring compared with HFD exposure during the gestation‑lactation period. The long‑term effect on follicular growth and development may be associated with increased oxidative stress and the activation of the insulin/PI3K/Akt pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yan S, Wang F and Shi Q: The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring. Exp Ther Med 20: 135, 2020.
APA
Yan, S., Wang, F., & Shi, Q. (2020). The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring. Experimental and Therapeutic Medicine, 20, 135. https://doi.org/10.3892/etm.2020.9264
MLA
Yan, S., Wang, F., Shi, Q."The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring". Experimental and Therapeutic Medicine 20.6 (2020): 135.
Chicago
Yan, S., Wang, F., Shi, Q."The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring". Experimental and Therapeutic Medicine 20, no. 6 (2020): 135. https://doi.org/10.3892/etm.2020.9264
Copy and paste a formatted citation
x
Spandidos Publications style
Yan S, Wang F and Shi Q: The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring. Exp Ther Med 20: 135, 2020.
APA
Yan, S., Wang, F., & Shi, Q. (2020). The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring. Experimental and Therapeutic Medicine, 20, 135. https://doi.org/10.3892/etm.2020.9264
MLA
Yan, S., Wang, F., Shi, Q."The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring". Experimental and Therapeutic Medicine 20.6 (2020): 135.
Chicago
Yan, S., Wang, F., Shi, Q."The effect of maternal high‑fat‑diet mediated oxidative stress on ovarian function in mice offspring". Experimental and Therapeutic Medicine 20, no. 6 (2020): 135. https://doi.org/10.3892/etm.2020.9264
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