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Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation

  • Authors:
    • Na Wang
    • Miaomiao Mi
    • Xiaonan Wei
    • Chengming Sun
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Center, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Laboratory Center Qingdao University, Qingdao, Shandong 266000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 147
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    Published online on: October 5, 2020
       https://doi.org/10.3892/etm.2020.9276
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Abstract

Circadian clock genes (CCGs) are reported to serve pivotal roles in regulating the development of certain tumors, including lung cancer and colon cancer . However, their expression patterns and function in chronic myeloid leukemia (CML) remains poorly understood. The present study aimed to investigate the expression and function of circadian clock gene Period2 (Per2) in human CML. Per2 expression levels in neutrophils isolated from patients with CML and healthy donors were measured via reverse transcription‑quantitative PCR. Subsequently, through lentivirus transduction, Per2 was stably overexpressed in human CML cell line KCL22 cells, which were injected into nude mice to investigate the in vivo role of Per2 by measuring CML tumor size and weight. Additionally, Per2 expression levels in patients with acute myeloid leukemia (AML) or chronic lymphocytic leukemia (CLL) were analyzed by re‑analyzing microarray data in the Gene Expression Omnibus database. Per2 expression was significantly lower in neutrophils isolated from patients with CML patients compared with healthy donors, and was negatively correlated with the expression level of c‑Myc. Similarly, patients with AML or CLL displayed lower Per2 expression levels compared with healthy controls. Per2 overexpression inhibited KCL22 cell proliferation in nude mice and in vitro, and induced cell cycle arrest at the G1 phase. By contrast, the results also indicated that KCL22 cell apoptosis was not regulated by Per2. The present study identified Per2 as a potential tumor suppressor in human CML.
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Copy and paste a formatted citation
Spandidos Publications style
Wang N, Mi M, Wei X and Sun C: Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation. Exp Ther Med 20: 147, 2020.
APA
Wang, N., Mi, M., Wei, X., & Sun, C. (2020). Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation. Experimental and Therapeutic Medicine, 20, 147. https://doi.org/10.3892/etm.2020.9276
MLA
Wang, N., Mi, M., Wei, X., Sun, C."Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation". Experimental and Therapeutic Medicine 20.6 (2020): 147.
Chicago
Wang, N., Mi, M., Wei, X., Sun, C."Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation". Experimental and Therapeutic Medicine 20, no. 6 (2020): 147. https://doi.org/10.3892/etm.2020.9276
Copy and paste a formatted citation
x
Spandidos Publications style
Wang N, Mi M, Wei X and Sun C: Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation. Exp Ther Med 20: 147, 2020.
APA
Wang, N., Mi, M., Wei, X., & Sun, C. (2020). Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation. Experimental and Therapeutic Medicine, 20, 147. https://doi.org/10.3892/etm.2020.9276
MLA
Wang, N., Mi, M., Wei, X., Sun, C."Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation". Experimental and Therapeutic Medicine 20.6 (2020): 147.
Chicago
Wang, N., Mi, M., Wei, X., Sun, C."Circadian clock gene Period2 suppresses human chronic myeloid leukemia cell proliferation". Experimental and Therapeutic Medicine 20, no. 6 (2020): 147. https://doi.org/10.3892/etm.2020.9276
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