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Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis

  • Authors:
    • Liping Qi
    • Kui Wu
    • Shutian Shi
    • Qingwei Ji
    • Huangtai Miao
    • Que Bin
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Clinical Center, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Lab for Cardiovascular Precision Medicine, Beijing 100029, P.R. China
    Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 150
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    Published online on: October 6, 2020
       https://doi.org/10.3892/etm.2020.9279
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Abstract

Thrombospondin‑2 (TSP‑2) is an important extracellular matrix protein that is involved in a variety of cardiovascular diseases, including viral myocarditis and abdominal aortic aneurysm. The present study aimed to investigate TSP‑2 expression in patients with aortic dissection (AD). Aortas were obtained from patients with AD and healthy donors, and TSP‑2 expression level in all samples was measured by western blotting and immunofluorescence assays. Blood samples were also collected from patients with AD and non‑AD (NAD) subjects. Circulating TSP‑2, tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 levels in each sample were detected using ELISAs. In addition, the effect of TSP‑2 on angiotensin II (Ang II)‑induced smooth muscle cell (SMC) apoptosis was assessed in vitro. Compared with healthy donors, aortic TSP‑2 expression level was significantly increased in patients with AD. Furthermore, TSP‑2 was secreted primarily by SMCs, but also by endothelial cells. TSP‑2 plasma expression level was also elevated in patients with AD compared with non‑AD subjects. Furthermore, TSP‑2 serum expression level was positively correlated with TNF‑α and IL‑6 expression levels in patients with AD. In addition, recombinant mouse TSP‑2 treatment increased Bax mRNA expression and decreased Bcl2 mRNA expression in Ang II‑treated SMCs; however, the effects were reversed following treatment with the NF‑κB p65 signaling pathway inhibitor JSH‑23 or with the anti‑TNF‑α and anti‑IL‑6 neutralizing antibodies. The present study demonstrated that TSP‑2 expression was increased in the aortic tissues and plasma of patients with AD. These findings suggested that TSP‑2 may participate in the progression of AD by activating the NF‑κB p65 signaling pathway and amplifying the inflammatory response.
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Copy and paste a formatted citation
Spandidos Publications style
Qi L, Wu K, Shi S, Ji Q, Miao H and Bin Q: Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis. Exp Ther Med 20: 150, 2020.
APA
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., & Bin, Q. (2020). Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis. Experimental and Therapeutic Medicine, 20, 150. https://doi.org/10.3892/etm.2020.9279
MLA
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., Bin, Q."Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis". Experimental and Therapeutic Medicine 20.6 (2020): 150.
Chicago
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., Bin, Q."Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis". Experimental and Therapeutic Medicine 20, no. 6 (2020): 150. https://doi.org/10.3892/etm.2020.9279
Copy and paste a formatted citation
x
Spandidos Publications style
Qi L, Wu K, Shi S, Ji Q, Miao H and Bin Q: Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis. Exp Ther Med 20: 150, 2020.
APA
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., & Bin, Q. (2020). Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis. Experimental and Therapeutic Medicine, 20, 150. https://doi.org/10.3892/etm.2020.9279
MLA
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., Bin, Q."Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis". Experimental and Therapeutic Medicine 20.6 (2020): 150.
Chicago
Qi, L., Wu, K., Shi, S., Ji, Q., Miao, H., Bin, Q."Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis". Experimental and Therapeutic Medicine 20, no. 6 (2020): 150. https://doi.org/10.3892/etm.2020.9279
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