Open Access

miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway

  • Authors:
    • Yiming Deng
    • Jingyu Zhang
    • Xuan Sun
    • Gaoting Ma
    • Gang Luo
    • Zhongrong Miao
    • Ligang Song
  • View Affiliations

  • Published online on: October 7, 2020     https://doi.org/10.3892/etm.2020.9288
  • Article Number: 159
  • Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Alzheimer's disease (AD) is a common worldwide progressive neurodegenerative disease. The dysregulation of miRNA is crucial in neurodegenerative diseases and neuron apoptosis during AD and is closely associated with the MAPK pathway. By bioinformatic website, we found that there was target inhibiting relationship between microRNA (miR)‑132 and MAPK1. Therefore, the current study speculated that miR‑132 could improve the cognitive function of rats with AD by inhibiting MAPK1 expression. To verify our hypothesis, 10 normal rats and 60 rats with AD were selected and divided into model, Ad‑miR‑132 negative control (NC), Ad‑miR‑132, Ad‑small interfering (si)MAPK1 NC, Ad‑siMAPK1 and Ad‑miR‑132 + Ad‑MAPK1 groups. Rats were evaluated for learning by performing morris water maze tests and pathological changes of the hippocampus were assessed via HE staining. Additionally, hippocampus cell apoptosis was determined using a TUNEL assay and levels of acetylcholinesterase (AChE), reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH‑Px) were evaluated in sera via ELISA. The mRNA and protein expression of miR‑132, iNOS, MAPK1 and phosphorylated (p)‑MAPK1 was determined in hippocampus tissues via reverse transcription‑quantitative PCR and western blotting, respectively. Compared with normal mice, rats with AD had significantly decreased learning abilities, increased cell apoptosis rates, increased levels of AChE, iNOS, ROS, MDA, MAPK1 and p‑MAPK1 and decreased levels of SOD, GSH‑Px and miR‑132. Upregulation of miR‑132 group improved the above indictors and silencing MAKP1 worsened the condition of rats. miR‑132 upregulation therefore reversed the negative effects caused by MAPK1 silencing in rats with AD. In conclusion, miR‑132 inhibited hippocampal iNOS expression and oxidative stress by inhibiting MAPK1expression to improve the cognitive function of rats with AD.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Deng Y, Zhang J, Sun X, Ma G, Luo G, Miao Z and Song L: miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway. Exp Ther Med 20: 159, 2020
APA
Deng, Y., Zhang, J., Sun, X., Ma, G., Luo, G., Miao, Z., & Song, L. (2020). miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway. Experimental and Therapeutic Medicine, 20, 159. https://doi.org/10.3892/etm.2020.9288
MLA
Deng, Y., Zhang, J., Sun, X., Ma, G., Luo, G., Miao, Z., Song, L."miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway". Experimental and Therapeutic Medicine 20.6 (2020): 159.
Chicago
Deng, Y., Zhang, J., Sun, X., Ma, G., Luo, G., Miao, Z., Song, L."miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway". Experimental and Therapeutic Medicine 20, no. 6 (2020): 159. https://doi.org/10.3892/etm.2020.9288