Open Access

Small interfering RNA‑mediated knockdown of KRT80 suppresses colorectal cancer proliferation

  • Authors:
    • Jiatian Lin
    • Xiaoqin Fan
    • Junhui Chen
    • Xina Xie
    • Hongjian Yu
  • View Affiliations

  • Published online on: October 9, 2020     https://doi.org/10.3892/etm.2020.9306
  • Article Number: 176
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is the third most common cancer in the world and its development is associated with oncogenic dysfunction. Therefore, the present study aimed to identify differentially expressed genes (DEGs) in CRC tissues and to determine the role of keratin 80 (KRT80) in CRC cell proliferation. DEGs were initially screened in 32 paired CRC tissues and matched adjacent normal tissues from RNA‑Seq datasets in The Cancer Genome Atlas database using the limma package in R software. In total, 2,114 DEGs were identified, of which KRT80 was discovered to be the most upregulated in CRC tissues. Moreover, increased KRT80 expression levels were confirmed in tissues collected from 50 patients with CRC using reverse transcription‑quantitative PCR, and its increased expression levels were significantly associated with increased lymph node and distant metastasis and a higher pathological stage. Furthermore, KRT80 knockdown using siRNA decreased the viability and proliferation of CRC cells. Finally, pathway analysis revealed that the proteins co‑expressed with KRT80 in CRC were enriched in the cell cycle, DNA replication, immune system, metabolism of protein and RNA, signal transduction and other cellular processes. Among them, the cell cycle and DNA replication pathways contained the highest number of the proteins identified. In conclusion, the findings of the present study suggested that KRT80 may be overexpressed in CRC tissues. Furthermore, KRT80 may be involved in the proliferation of CRC cells, which is likely through its ability to regulate the cell cycle and DNA replication pathways, thus it may serve as a potential therapeutic target for patients with CRC.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lin J, Fan X, Chen J, Xie X and Yu H: Small interfering RNA‑mediated knockdown of KRT80 suppresses colorectal cancer proliferation. Exp Ther Med 20: 176, 2020
APA
Lin, J., Fan, X., Chen, J., Xie, X., & Yu, H. (2020). Small interfering RNA‑mediated knockdown of KRT80 suppresses colorectal cancer proliferation. Experimental and Therapeutic Medicine, 20, 176. https://doi.org/10.3892/etm.2020.9306
MLA
Lin, J., Fan, X., Chen, J., Xie, X., Yu, H."Small interfering RNA‑mediated knockdown of KRT80 suppresses colorectal cancer proliferation". Experimental and Therapeutic Medicine 20.6 (2020): 176.
Chicago
Lin, J., Fan, X., Chen, J., Xie, X., Yu, H."Small interfering RNA‑mediated knockdown of KRT80 suppresses colorectal cancer proliferation". Experimental and Therapeutic Medicine 20, no. 6 (2020): 176. https://doi.org/10.3892/etm.2020.9306