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Immune cell infiltration and related core genes expression characteristics in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps

  • Authors:
    • Gaoyun Xiong
    • Xiaoxing Xie
    • Qingliang Wang
    • Yanyan Zhang
    • Yanping Ge
    • Wei Lin
    • Mingqian Li
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  • Published online on: October 12, 2020     https://doi.org/10.3892/etm.2020.9310
  • Article Number: 180
  • Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic rhinosinusitis with nasal polyps (CRSwNP) refers to chronic inflammation of the sinonasal mucosa. It can either be eosinophilic (ECRSwNP) or non‑eosinophilic (non‑ECRSwNP). However, immune cell infiltration in the microenvironment and pathogenesis of ECRSwNP and non‑ECRSwNP are still unclear. The aim of the present study was to assess the immune cell infiltration and molecular mechanisms of ECRSwNP and non‑ECRSwNP. In the present study, 22 immune cell types in ECRSwNP and non‑ECRSwNP were investigated by CIBERSORT based on transcriptome data. The core gene related pathophysiology of CRSwNP was analyzed using Weighted Gene Correlation Network Analysis according to the phenotype of the infiltrated eosinophils and nasal polyps (NP). A total of four types of immune cells (mast cells, activated dendritic cells, M2 macrophages and activated natural killer cells) were demonstrated to have a direct and indirect correlation with eosinophilic infiltration in ECRSwNP. M1 macrophages and activated CD4+ memory T cells were correlated with major immune cell types in non‑ECRSwNP. NP could affect the expression of ‘olfactory receptor activity’ and ‘channel activity’ genes to impair the olfactory signaling pathway and neuroactive ligand receptor pathway. ‘Cell adhesion molecule binding’, ‘cytokine receptor binding’ and ‘glucocorticoid receptor binding’ were significantly enriched in ECRSwNP, whereas epithelial cell injury, autophagy and the mTOR pathway (hsa04140 and hsa04150) may serve an important role in the pathogenesis of non‑ECRSwNP. There were significantly different immune cell infiltration and related core genes expression characteristics between ECRSwNP and non‑ECRSwNP. The results of the present study provide an improved basis for elucidation of the mechanism and treatment of CRSwNP.
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Spandidos Publications style
Xiong G, Xie X, Wang Q, Zhang Y, Ge Y, Lin W and Li M: Immune cell infiltration and related core genes expression characteristics in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Exp Ther Med 20: 180, 2020
APA
Xiong, G., Xie, X., Wang, Q., Zhang, Y., Ge, Y., Lin, W., & Li, M. (2020). Immune cell infiltration and related core genes expression characteristics in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps. Experimental and Therapeutic Medicine, 20, 180. https://doi.org/10.3892/etm.2020.9310
MLA
Xiong, G., Xie, X., Wang, Q., Zhang, Y., Ge, Y., Lin, W., Li, M."Immune cell infiltration and related core genes expression characteristics in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Experimental and Therapeutic Medicine 20.6 (2020): 180.
Chicago
Xiong, G., Xie, X., Wang, Q., Zhang, Y., Ge, Y., Lin, W., Li, M."Immune cell infiltration and related core genes expression characteristics in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps". Experimental and Therapeutic Medicine 20, no. 6 (2020): 180. https://doi.org/10.3892/etm.2020.9310