lncRNA ZEB1‑AS1 is downregulated in diabetic lung and regulates lung cell apoptosis

  • Authors:
    • Lizhi Gu
    • Hong Sun
    • Zhuan Yan
  • View Affiliations

  • Published online on: October 15, 2020     https://doi.org/10.3892/etm.2020.9355
  • Article Number: 225
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Abstract

The present study aimed to investigate the role of ZEB1‑antisense RNA 1 (AS1) in diabetic lung (pneumonia with excluded causes other than diabetes). In the present study, the expression of ZEB1‑AS1 in plasma was detected by performing reverse transcription‑quantitative PCR. A receiver operating characteristic curve was used for diagnostic analysis. Lung cell apoptosis under the treatment of high glucose was analyzed by a cell apoptosis assay. p53 expression in lung cells was detected by performing western blotting. The present data demonstrated that ZEB1‑AS1 was downregulated in the plasma of patients with diabetic lung (DL) compared with diabetic patients without complications (~1.6‑fold) and healthy controls (~2.4‑fold), and downregulation of ZEB1‑AS1 distinguished patients with DL from healthy controls. ZEB1‑AS1 in lung cells was downregulated by high glucose treatment, and overexpression of ZEB1‑AS1 resulted in inhibited lung cancer cell apoptosis and downregulated p53. p53 overexpression attenuated the effects of ZEB1‑AS1 overexpression on lung cell apoptosis. In conclusion, the present study demonstrated that ZEB1‑AS1 was downregulated in patients with DL and regulates lung cancer cell apoptosis by downregulating p53.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gu L, Sun H and Yan Z: lncRNA ZEB1‑AS1 is downregulated in diabetic lung and regulates lung cell apoptosis. Exp Ther Med 20: 225, 2020
APA
Gu, L., Sun, H., & Yan, Z. (2020). lncRNA ZEB1‑AS1 is downregulated in diabetic lung and regulates lung cell apoptosis. Experimental and Therapeutic Medicine, 20, 225. https://doi.org/10.3892/etm.2020.9355
MLA
Gu, L., Sun, H., Yan, Z."lncRNA ZEB1‑AS1 is downregulated in diabetic lung and regulates lung cell apoptosis". Experimental and Therapeutic Medicine 20.6 (2020): 225.
Chicago
Gu, L., Sun, H., Yan, Z."lncRNA ZEB1‑AS1 is downregulated in diabetic lung and regulates lung cell apoptosis". Experimental and Therapeutic Medicine 20, no. 6 (2020): 225. https://doi.org/10.3892/etm.2020.9355