Rapid method for direct identification of positive blood cultures by MALDI‑TOF MS

  • Authors:
    • Yueling Wang
    • Yan Jin
    • Yuanyuan Bai
    • Zhen Song
    • Wenjun Chu
    • Mengqi Zhao
    • Yingying Hao
    • Zhiming Lu
  • View Affiliations

  • Published online on: October 16, 2020     https://doi.org/10.3892/etm.2020.9365
  • Article Number: 235
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Abstract

Application of matrix‑assisted laser desorption ionization‑time of flight mass spectrometry (MALDI‑TOF MS) using positive blood cultures (BCs) is a revolution in identification of microorganisms in clinical microbiology laboratories. Although there are several commercial pretreatment protocols they are expensive. Here, we evaluated the performance of a locally produced Bioyong pre‑treatment kit for the direct identification of microorganisms in positive BCs by MALDI‑TOF MS method. The mocked positive BCs were performed using 200 Thermo aerobic blood culture bottles and 200 aerobic Scenker blood culture bottles. A total of 200 organisms were invovled, including 91 strains of Gram‑positive bacteria, 97 strains of Gram‑negative bacteria and 12 strains of Candida. The positive BCs were subcultured and identified by classical biochemical Vitek II testing as the gold standard of identification. The Bioyong pre‑treatment kit could successfully identify microorganisms in 189 (94.5%) Thermo positive BCs and 189 (94.5%) Scenker positive blood cultures, respectively. In total, 94 (96.9%) Gram‑negative bacteria, 86 (94.5%) Gram‑positive bacteria and 9 (75.0%) candida isolated from Thermo positive BCs were correctly identified to species level and 95 (97.9%) Gram‑negative bacteria, 86 (94.5%) Gram‑positive bacteria and 8 (66.7%) candida isolated from Scenker positive BCs were correctly identified to species level. This method provides a rapid, accurate identification of bacteria and Candida within one hour in positive blood cultures. Routine application of this technique will improve the antimicrobial treatment within 24 h among the patients with bacteremia and candidemia.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang Y, Jin Y, Bai Y, Song Z, Chu W, Zhao M, Hao Y and Lu Z: Rapid method for direct identification of positive blood cultures by MALDI‑TOF MS. Exp Ther Med 20: 235, 2020
APA
Wang, Y., Jin, Y., Bai, Y., Song, Z., Chu, W., Zhao, M. ... Lu, Z. (2020). Rapid method for direct identification of positive blood cultures by MALDI‑TOF MS. Experimental and Therapeutic Medicine, 20, 235. https://doi.org/10.3892/etm.2020.9365
MLA
Wang, Y., Jin, Y., Bai, Y., Song, Z., Chu, W., Zhao, M., Hao, Y., Lu, Z."Rapid method for direct identification of positive blood cultures by MALDI‑TOF MS". Experimental and Therapeutic Medicine 20.6 (2020): 235.
Chicago
Wang, Y., Jin, Y., Bai, Y., Song, Z., Chu, W., Zhao, M., Hao, Y., Lu, Z."Rapid method for direct identification of positive blood cultures by MALDI‑TOF MS". Experimental and Therapeutic Medicine 20, no. 6 (2020): 235. https://doi.org/10.3892/etm.2020.9365