Open Access

MicroRNA‑210 improves perfusion recovery following hindlimb ischemia via suppressing reactive oxygen species

  • Authors:
    • Jinfeng Zhang
    • Guotao Rao
    • Junying Qiu
    • Ronghua He
    • Qiongtao Wang
  • View Affiliations

  • Published online on: October 16, 2020     https://doi.org/10.3892/etm.2020.9366
  • Article Number: 236
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In peripheral arterial disease (PAD), angiogenesis is a major process involved in repairing the microvasculature in the ischemic lower limb. MicroRNA‑210 (miR‑210) is a microRNA that is substantially increased in patients with PAD. However, the effects of miR‑210 on angiogenesis following PAD remain elusive. In the present study, mice with hindlimb ischemia (HLI) were generated as an animal model of PAD, and miR‑210 levels were overexpressed in the ischemic limb. The overexpression of miR‑210 using microRNA mimics greatly improved angiogenesis and perfusion recovery; in contrast, the knockdown of miR‑210 impaired perfusion recovery 28 days after HLI. Ischemic muscle tissue was harvested 7 days after experimental PAD in order to perform biochemical tests, and miR‑210 antagonism resulted in increased malondialdehyde levels. In cultured endothelial cells under simulated ischemia, miR‑210 mimic improved endothelial cell viability and enhanced tube formation; and a miR‑210 inhibitor decreased cell survival, reduced tube formation and increased reactive oxygen species (ROS) levels. Furthermore, miR‑210 antagonism increased the protein disulfide‑isomerase levels in cultured endothelial cells. These results demonstrate that ischemia‑induced miR‑210 elevation is adaptive in PAD, and that miR‑210 improves angiogenesis at least partially through decreasing ROS production.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang J, Rao G, Qiu J, He R and Wang Q: MicroRNA‑210 improves perfusion recovery following hindlimb ischemia via suppressing reactive oxygen species. Exp Ther Med 20: 236, 2020
APA
Zhang, J., Rao, G., Qiu, J., He, R., & Wang, Q. (2020). MicroRNA‑210 improves perfusion recovery following hindlimb ischemia via suppressing reactive oxygen species. Experimental and Therapeutic Medicine, 20, 236. https://doi.org/10.3892/etm.2020.9366
MLA
Zhang, J., Rao, G., Qiu, J., He, R., Wang, Q."MicroRNA‑210 improves perfusion recovery following hindlimb ischemia via suppressing reactive oxygen species". Experimental and Therapeutic Medicine 20.6 (2020): 236.
Chicago
Zhang, J., Rao, G., Qiu, J., He, R., Wang, Q."MicroRNA‑210 improves perfusion recovery following hindlimb ischemia via suppressing reactive oxygen species". Experimental and Therapeutic Medicine 20, no. 6 (2020): 236. https://doi.org/10.3892/etm.2020.9366