Open Access

Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis

  • Authors:
    • Shengdan Zhang
    • Yulong Niu
    • Zhuo Yang
    • Yuwei Zhang
    • Qiang Guo
    • Yi Yang
    • Xuedong Zhou
    • Yi Ding
    • Chengcheng Liu
  • View Affiliations

  • Published online on: October 23, 2020     https://doi.org/10.3892/etm.2020.9381
  • Article Number: 251
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Biochanin A (BA) is an organic compound produced by Trifolium pretense and Arachis hypogaea with anti‑inflammatory and antioxidative effects. The aim of the current study was to evaluate the effects of BA on gingival inflammation and alveolar bone destruction in rats with experimental periodontitis. Experimental rats (n=25) were distributed equally into five groups: i) Healthy control (control) group; ii) experimental periodontitis (ligation) group; and iii) and ligation plus low, medium and high dose of BA (12.5, 25 and 50 mg/kg/day, respectively) groups. A nylon ligature was inserted around rats' maxillary molars for 14 days to trigger the experimental periodontitis. BA was intravenous injected once daily for 4 weeks. After that, interleukin‑1β (IL‑1β), tumor necrosis factor‑α (TNF‑α), reactive oxygen species (ROS) and osteocalcin (OCN) levels were determined in gingival and/or serum samples using ELISA or reverse transcription‑quantitative PCR. Alveolar bone volume was assessed via hematoxylin and eosin staining and micro‑computed tomography. Osteoclasts were identified by tartrate‑resistant acid phosphatase staining, and the level of the nuclear factor erythroid‑2 related factor 2 (Nrf2) was also detected by immunohistochemical staining. BA treatment groups showed alleviated alveolar bone resorption compared with the ligation group. Moreover, BA treatment significantly inhibited IL‑1β, TNF‑α, ROS levels, and reduced leukocyte acid phosphatase‑positive cells, as well as increased OCN and Nrf2 levels compared with the ligation group. BA had beneficial effects on experimental periodontitis of rats. BA treatment inhibited inflammation, regulated unbalanced oxidative stress response and ameliorated the alveolar bone loss.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang S, Niu Y, Yang Z, Zhang Y, Guo Q, Yang Y, Zhou X, Ding Y and Liu C: Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis. Exp Ther Med 20: 251, 2020
APA
Zhang, S., Niu, Y., Yang, Z., Zhang, Y., Guo, Q., Yang, Y. ... Liu, C. (2020). Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis. Experimental and Therapeutic Medicine, 20, 251. https://doi.org/10.3892/etm.2020.9381
MLA
Zhang, S., Niu, Y., Yang, Z., Zhang, Y., Guo, Q., Yang, Y., Zhou, X., Ding, Y., Liu, C."Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis". Experimental and Therapeutic Medicine 20.6 (2020): 251.
Chicago
Zhang, S., Niu, Y., Yang, Z., Zhang, Y., Guo, Q., Yang, Y., Zhou, X., Ding, Y., Liu, C."Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis". Experimental and Therapeutic Medicine 20, no. 6 (2020): 251. https://doi.org/10.3892/etm.2020.9381