Open Access

Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p

  • Authors:
    • Xianming Liu
    • Cunchuan Wang
  • View Affiliations

  • Published online on: October 27, 2020     https://doi.org/10.3892/etm.2020.9391
  • Article Number: 261
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) serve crucial roles in cancer development and progression. lncRNA‑activated by transforming growth factor‑β (lncRNA‑ATB) mediates cell proliferation. However, the association between lncRNA‑ATB and human colorectal cancer (CRC) is not completely understood. Therefore, the present study aimed to investigate the role of lncRNA‑ATB in CRC, as well as the underlying mechanism. 50 pairs of tumor tissues and adjacent normal tissues from patients with primary CRC were collected. The expression of lncRNA‑ATB and microRNA (miR)‑141‑3p in CRC tissues, adjacent normal tissues and cell lines was detected using reverse transcription‑quantitative PCR. CCK‑8, colony formation, Transwell, western blot, dual luciferase reporter gene, RNA immunoprecipitation and immunohistochemistry staining assays were conducted to assess the biological function of lncRNA‑ATB and miR‑141‑3p in CRC progression. lncRNA‑ATB was upregulated in CRC tissues and cell lines compared with healthy tissues and cells, respectively. Moreover, high expression of lncRNA‑ATB was significantly associated with advanced TNM stage and metastasis in CRC. In addition, the results indicated that lncRNA‑ATB expression predicted the prognosis and overall survival of patients with CRC. Compared with small interfering RNA‑negative control, lncRNA‑ATB knockdown inhibited CRC cell proliferation, migration and invasion, whereas, compared with vector, lncRNA‑ATB overexpression promoted CRC cell proliferation, migration and invasion. Furthermore, the in vivo experiment suggested that lncRNA‑ATB knockdown inhibited tumor growth. The results also indicated that lncRNA‑ATB may contribute to CRC progression via binding to tumor suppressor microRNA‑141‑3p. Collectively, the present study suggested a crucial role of lncRNA‑ATB in CRC tumorigenesis, suggesting that lncRNA‑ATB may serve as an important marker for the diagnosis and development of CRC.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu X and Liu X: Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p. Exp Ther Med 20: 261, 2020
APA
Liu, X., & Liu, X. (2020). Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p. Experimental and Therapeutic Medicine, 20, 261. https://doi.org/10.3892/etm.2020.9391
MLA
Liu, X., Wang, C."Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p". Experimental and Therapeutic Medicine 20.6 (2020): 261.
Chicago
Liu, X., Wang, C."Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p". Experimental and Therapeutic Medicine 20, no. 6 (2020): 261. https://doi.org/10.3892/etm.2020.9391