Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2020 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2020 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway

  • Authors:
    • Wei Xie
    • Zhuqing Wang
    • Yanbo Zhang
    • Zhenpeng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Sport Medicine, Traditional Chinese Medicine Hospital of Weifang, Weifang, Shandong 261041, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 271
    |
    Published online on: October 27, 2020
       https://doi.org/10.3892/etm.2020.9401
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Although fragility fracture is a global public health burden, the mechanisms underlying fracture healing remain unclear. The present study aimed to assess the dynamic expression pattern of microRNA‑328‑3p (miR‑328‑3p) during fracture healing in patients with fragility fracture and to explore the functional role and mechanisms of miR‑328‑3p in the regulation of osteoblastic viability. The expression levels of miR‑328‑3p was examined using reverse transcription‑quantitative PCR (RT‑qPCR). Osteoblastic proliferation and apoptosis were analyzed via MTT and flow cytometry assays. A luciferase reporter assay was adopted to confirm the interaction between miR‑328‑3p and its target gene PTEN, and western blotting was used to explore the activity of PI3K/AKT signaling. The results of the present study demonstrated that serum miR‑328‑3p expression did not significantly differ at the early stage of healing in patients with fracture, but was markedly decreased 14 and 21 days post fixation (P<0.01). PTEN was demonstrated to be a target gene of miR‑328‑3p and was inhibited by miR‑328‑3p overexpression in osteoblasts (P<0.001). miR‑328‑3p overexpression increased osteoblastic proliferation but decreased apoptotic rate, with these effects being reversed by PTEN overexpression (P<0.05). The expression of phosphorylated‑AKT was elevated in osteoblasts by miR‑328‑3p overexpression, but this effect was abolished by overexpressing PTEN. Thus, the present study revealed that miR‑328‑3p may accelerate fracture healing by promoting osteoblastic viability through the PTEN/PI3K/AKT pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Dreinhofer KE, Mitchell PJ, Bégué T, Cooper C, Costa ML, Falaschi P, Hertz K, Marsh D, Maggi S, Nana A, et al: A global call to action to improve the care of people with fragility fractures. Injury. 49:1393–1397. 2018.PubMed/NCBI View Article : Google Scholar

2 

Horowitz JA, Puvanesarajah V, Jain A, Raad M, Gjolaj JP, Shen FH and Hassanzadeh H: Fragility fracture risk in elderly patients with cervical myelopathy. Spine (Phila Pa 1976). 44:96–102. 2019.PubMed/NCBI View Article : Google Scholar

3 

Friedman SM and Mendelson DA: Epidemiology of fragility fractures. Clin Geriatr Med. 30:175–181. 2014.PubMed/NCBI View Article : Google Scholar

4 

Wasserman H, Backeljauw PF, Khoury JC, Kalkwarf HJ and Gordon CM: Bone fragility in Turner syndrome: Fracture prevalence and risk factors determined by a national patient survey. Clin Endocrinol (Oxf). 89:46–55. 2018.PubMed/NCBI View Article : Google Scholar

5 

Oryan A, Monazzah S and Bigham-Sadegh A: Bone injury and fracture healing biology. Biomed Environ Sci. 28:57–71. 2015.PubMed/NCBI View Article : Google Scholar

6 

Cheng C and Shoback D: Mechanisms underlying normal fracture healing and risk factors for delayed healing. Curr Osteoporos Rep. 17:36–47. 2019.PubMed/NCBI View Article : Google Scholar

7 

Mohr AM and Mott JL: Overview of microRNA biology. Semin Liver Dis. 35:3–11. 2015.PubMed/NCBI View Article : Google Scholar

8 

Wang CZ, Deng F, Li H, Wang DD, Zhang W, Ding L and Tang JH: MiR-101: A potential therapeutic target of cancers. Am J Transl Res. 10:3310–3321. 2018.PubMed/NCBI

9 

Zhang B, Yi J, Zhang CL, Zhang QH, Xu JF, Shen HQ and Ge DW: MiR-146a inhibits proliferation and induces apoptosis in murine osteoblastic MC3T3-E1 by regulating Bcl2. Eur Rev Med Pharmacol Sci. 21:3754–3762. 2017.PubMed/NCBI

10 

Li X, Li K, Yu G, Yu C and Liu C: miR-342-5p inhibits expression of Bmp7 to regulate proliferation, differentiation and migration of osteoblasts. Mol Immunol. 114:251–259. 2019.PubMed/NCBI View Article : Google Scholar

11 

Tu XM, Gu YL and Ren GQ: miR-125a-3p targetedly regulates GIT1 expression to inhibit osteoblastic proliferation and differentiation. Exp Ther Med. 12:4099–4106. 2016.PubMed/NCBI View Article : Google Scholar

12 

Hu WX, Li H and Jiang JZ: MiR-491-3p is down-regulated in postmenopausal osteoporosis and affects growth, differentiation and apoptosis of hFOB1.19 cells through targeting CTSS. Folia Histochem Cytobiol. 1:9–16. 2020.PubMed/NCBI View Article : Google Scholar

13 

Pan S, Ren F, Li L, Liu D, Li Y, Wang A, Li W, Dong Y and Guo W: MiR-328-3p inhibits cell proliferation and metastasis in colorectal cancer by targeting Girdin and inhibiting the PI3K/Akt signaling pathway. Exp Cell Res. 390(111939)2020.PubMed/NCBI View Article : Google Scholar

14 

Yi W, Tu MJ, Liu Z, Zhang C, Batra N, Yu AX and Yu AM: Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics. Acta Pharm Sin B. 10:159–170. 2020.PubMed/NCBI View Article : Google Scholar

15 

Weilner S, Skalicky S, Salzer B, Keider V, Wagner M, Hildner F, Gabriel C, Dovjak P, Pietschmann P, Grillari-Voglauer R, et al: Differentially circulating miRNAs after recent osteoporotic fractures can influence osteogenic differentiation. Bone. 79:43–51. 2015.PubMed/NCBI View Article : Google Scholar

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

17 

Malaney P, Uversky VN and Dave V: PTEN proteoforms in biology and disease. Cell Mol Life Sci. 74:2783–2794. 2017.PubMed/NCBI View Article : Google Scholar

18 

Li D, Li L, Chen X, Gao Y, Cao Y and Hao B: LncRNA GHET1 promotes osteoblast proliferation and differentiation by inhibiting PTEN. Panminerva Med Jul 29, 2019 (Online ahead of print).

19 

Xu W, Yang Z, Xie C, Zhu Y, Shu X, Zhang Z, Li N, Chai N, Zhang S, Wu K, et al: PTEN lipid phosphatase inactivation links the hippo and PI3K/Akt pathways to induce gastric tumorigenesis. J Exp Clin Cancer Res. 37(198)2018.PubMed/NCBI View Article : Google Scholar

20 

Einhorn TA and Gerstenfeld LC: Fracture healing: Mechanisms and interventions. Nat Rev Rheumatol. 11:45–54. 2015.PubMed/NCBI View Article : Google Scholar

21 

Zhao C, Zhou Y, Ran Q, Yao Y, Zhang H, Ju J, Yang T, Zhang W, Yu X and He S: MicroRNA-381-3p functions as a dual suppressor of apoptosis and necroptosis and promotes proliferation of renal cancer cells. Front Cell Dev Biol. 8(290)2020.PubMed/NCBI View Article : Google Scholar

22 

Lang Y, Sun Q, Zhu LM, Qiu XD, Hu BS, Yang J and Zhang JD: MiR-25 overexpression promotes fracture healing by activating the Wnt signaling pathway. Eur Rev Med Pharmacol Sci. 23:7200–7208. 2019.PubMed/NCBI View Article : Google Scholar

23 

Mi B, Xiong Y, Chen L, Yan C, Endo Y, Liu Y, Liu J, Hu L, Hu Y, Sun Y, et al: CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis. Aging (Albany NY). 11:11988–12001. 2019.PubMed/NCBI View Article : Google Scholar

24 

Zhang SY, Gao F, Peng CG, Zheng CJ and Wu MF: Hsa-miR-203 inhibits fracture healing via targeting PBOV1. Eur Rev Med Pharmacol Sci. 22:5797–5803. 2018.PubMed/NCBI View Article : Google Scholar

25 

Shi J, An G, Guan Y, Wei T, Peng Z, Liang M and Wang Y: miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16. Cancer Cell Int. 19(104)2019.PubMed/NCBI View Article : Google Scholar

26 

Li JZ, Li J and Liu BZ: MicroRNA-328-3p inhibits malignant progression of hepatocellular carcinoma by regulating MMP-9 level. Eur Rev Med Pharmacol Sci. 23:9331–9340. 2019.PubMed/NCBI View Article : Google Scholar

27 

Rommens PM, Wagner D and Hofmann A: Fragility fractures of the pelvis. JBJS Rev. 5:01874474–201703000-00004. 2017.PubMed/NCBI View Article : Google Scholar

28 

Rossi LMM, Copes RM, Dal Osto LC, Flores C, Comim FV and Premaor MO: Factors related with osteoporosis treatment in postmenopausal women. Medicine (Baltimore). 97(e11524)2018.PubMed/NCBI View Article : Google Scholar

29 

Chen R, Liao X, Chen F, Wang B, Huang J, Jian G, Huang Z, Yin G, Liu H and Jin D: Circulating microRNAs, miR-10b-5p, miR-328-3p, miR-100 and let-7, are associated with osteoblast differentiation in osteoporosis. Int J Clin Exp Pathol. 11:1383–1390. 2018.PubMed/NCBI

30 

Zhou Y, Wang M, Zhang J, Xu P and Wang H: MicroRNA-29a-3p regulates abdominal aortic aneurysm development and progression via direct interaction with PTEN. J Cell Physiol. 235:9414–9423. 2020.PubMed/NCBI View Article : Google Scholar

31 

Zha X, Xi X, Fan X, Ma M, Zhang Y and Yang Y: Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8. Aging (Albany NY). 12:8137–8150. 2020.PubMed/NCBI View Article : Google Scholar

32 

Ge JB, Lin JT, Hong HY, Sun YJ, Li Y and Zhang CM: MiR-374b promotes osteogenic differentiation of MSCs by degrading PTEN and promoting fracture healing. Eur Rev Med Pharmacol Sci. 22:3303–3310. 2018.PubMed/NCBI View Article : Google Scholar

33 

Burgers TA, Hoffmann MF, Collins CJ, Zahatnansky J, Alvarado MA, Morris MR, Sietsema DL, Mason JJ, Jones CB, Ploeg HL and Williams BO: Mice lacking pten in osteoblasts have improved intramembranous and late endochondral fracture healing. PLoS One. 8(e63857)2013.PubMed/NCBI View Article : Google Scholar

34 

Liu Y, Liu J, Xia T, Mi BB, Xiong Y, Hu LC, Ruan TY, Zhou W and Liu GH: MiR-21 promotes fracture healing by activating the PI3K/Akt signaling pathway. Eur Rev Med Pharmacol Sci. 23:2727–2733. 2019.PubMed/NCBI View Article : Google Scholar

35 

Sun Z, Liu F, Cai X, Yu W, Xu L and Yang B: MiR-126 affects femoral fracture healing in rats through PI3K/AKT signaling pathway. Panminerva Med Jun 28, 2019.

36 

Yao LZ, Zhu YL and Liu JJ: Inhibition of PTEN gene expression by small interfering RNA on PI3K/Akt/FoxO3a signaling pathway in human nasopharyngeal carcinoma. Technol Cancer Res Treat 19: 1533033820917959, 2020.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xie W, Wang Z, Zhang Y and Zhang Z: Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway. Exp Ther Med 20: 271, 2020.
APA
Xie, W., Wang, Z., Zhang, Y., & Zhang, Z. (2020). Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway. Experimental and Therapeutic Medicine, 20, 271. https://doi.org/10.3892/etm.2020.9401
MLA
Xie, W., Wang, Z., Zhang, Y., Zhang, Z."Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway". Experimental and Therapeutic Medicine 20.6 (2020): 271.
Chicago
Xie, W., Wang, Z., Zhang, Y., Zhang, Z."Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway". Experimental and Therapeutic Medicine 20, no. 6 (2020): 271. https://doi.org/10.3892/etm.2020.9401
Copy and paste a formatted citation
x
Spandidos Publications style
Xie W, Wang Z, Zhang Y and Zhang Z: Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway. Exp Ther Med 20: 271, 2020.
APA
Xie, W., Wang, Z., Zhang, Y., & Zhang, Z. (2020). Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway. Experimental and Therapeutic Medicine, 20, 271. https://doi.org/10.3892/etm.2020.9401
MLA
Xie, W., Wang, Z., Zhang, Y., Zhang, Z."Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway". Experimental and Therapeutic Medicine 20.6 (2020): 271.
Chicago
Xie, W., Wang, Z., Zhang, Y., Zhang, Z."Beneficial role of microRNA‑328‑3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway". Experimental and Therapeutic Medicine 20, no. 6 (2020): 271. https://doi.org/10.3892/etm.2020.9401
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team