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Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits

  • Authors:
    • Huanxin Xie
    • Lei Cao
    • Linlin Ye
    • Jubao Du
    • Guixiang Shan
    • Jie Hu
    • Chunjing Jiang
    • Weiqun Song
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 279
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    Published online on: October 27, 2020
       https://doi.org/10.3892/etm.2020.9409
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Abstract

Long‑term bone defects are a key clinical problem. Autogenous bone graft remains the gold standard for the treatment of these defects; however, improving the osteogenic properties and reducing the amount of autogenous bone is challenging. Autologous platelet‑rich plasma (PRP) has been widely considered for treatment, due to its potentially beneficial effect on bone regeneration and vascularization. The aim of the present study was to explore the effects of autogenous bone particles combined with PRP on repairing segmental bone defects in rabbits. Briefly, a critical‑size diaphyseal radius defect was established in 45 New Zealand White rabbits. Animals were randomly divided into four groups, according to the different implants: Group A, empty bone defect; group B, PRP; group C, autogenous bone particles + bone mesenchymal stem cells (BMSCs) on the left radius; group D, autogenous bone particles + PRP + BMSCs on the right radius. Bone samples were collected and further analyzed using X‑ray, histology and histomorphometry 4, 8 and 12 weeks post‑surgery. In addition, the effect of PRP on cell proliferation was detected by Cell Counting Kit‑8 and the concentrations of growth factors (GFs), transforming GF (TGF)‑β1 and platelet‑derived GF (PDGF), in PRP were verified by ELISA. X‑ray, histology and histomorphometry data revealed that the fraction area of the newly formed bone was larger in group D. In addition, PRP could improve cell proliferation, osteogenic differentiation and the release of GFs, TGF‑β1 and PDGF‑AB. In conclusion, these findings indicated that an autogenous bone particle + PRP + BMSC scaffold may be used as a potential treatment strategy for segmental defects in humans.
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1 

Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R and Szalay K: The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials. 29:3983–3992. 2008.PubMed/NCBI View Article : Google Scholar

2 

Nooeaid P, Salih V, Beier JP and Boccaccini AR: Osteochondral tissue engineering: Scaffolds, stem cells and applications. J Cell Mol Med. 16:2247–2270. 2012.PubMed/NCBI View Article : Google Scholar

3 

Cheng X, Wan Q and Pei X: Graphene Family Materials in Bone Tissue Regeneration: Perspectives and Challenges. Nanoscale Res Lett. 13(289)2018.PubMed/NCBI View Article : Google Scholar

4 

Liao HT, Tsai MJ, Brahmayya M and Chen JP: Bone Regeneration Using Adipose-Derived Stem Cells in Injectable Thermo-Gelling Hydrogel Scaffold Containing Platelet-Rich Plasma and Biphasic Calcium Phosphate. Int J Mol Sci. 19(2537)2018.PubMed/NCBI View Article : Google Scholar

5 

Wen Y, Gu W, Cui J, Yu M, Zhang Y, Tang C, Yang P and Xu X: Platelet-rich plasma enhanced umbilical cord mesenchymal stem cells-based bone tissue regeneration. Arch Oral Biol. 59:1146–1154. 2014.PubMed/NCBI View Article : Google Scholar

6 

Ferrari M, Zia S, Valbonesi M, Henriquet F, Venere G, Spagnolo S, Grasso MA and Panzani I: A new technique for hemodilution, preparation of autologous platelet-rich plasma and intraoperative blood salvage in cardiac surgery. Int J Artif Organs. 10:47–50. 1987.PubMed/NCBI

7 

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE and Georgeff KR: Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 85:638–646. 1998.PubMed/NCBI View Article : Google Scholar

8 

Xie X, Wang Y, Zhao C, Guo S, Liu S, Jia W, Tuan RS and Zhang C: Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials. 33:7008–7018. 2012.PubMed/NCBI View Article : Google Scholar

9 

Son SR, Sarkar SK, Nguyen-Thuy BL, Padalhin AR, Kim BR, Jung HI and Lee BT: Platelet-rich plasma encapsulation in hyaluronic acid/gelatin-BCP hydrogel for growth factor delivery in BCP sponge scaffold for bone regeneration. J Biomater Appl. 29:988–1002. 2015.PubMed/NCBI View Article : Google Scholar

10 

Zou J, Yuan C, Wu C, Cao C and Yang H: The effects of platelet-rich plasma on the osteogenic induction of bone marrow mesenchymal stem cells. Connect Tissue Res. 55:304–309. 2014.PubMed/NCBI View Article : Google Scholar

11 

Chahla J, Cinque ME, Piuzzi NS, Mannava S, Geeslin AG, Murray IR, Dornan GJ, Muschler GF and LaPrade RF: A Call for Standardization in Platelet-Rich Plasma Preparation Protocols and Composition Reporting: A Systematic Review of the Clinical Orthopaedic Literature. J Bone Joint Surg Am. 99:1769–1779. 2017.PubMed/NCBI View Article : Google Scholar

12 

Oryan A, Alidadi S and Moshiri A: Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. 16:213–232. 2016.PubMed/NCBI View Article : Google Scholar

13 

Landesberg R, Burke A, Pinsky D, Katz R, Vo J, Eisig SB and Lu HH: Activation of platelet-rich plasma using thrombin receptor agonist peptide. J Maxillofac Surg. 63:529–535. 2005.PubMed/NCBI View Article : Google Scholar

14 

Oryan A, Alidadi S, Moshiri A and Maffulli N: Bone regenerative medicine: Classic options, novel strategies, and future directions. J Orthop Surg Res. 9(18)2014.PubMed/NCBI View Article : Google Scholar

15 

Duan R, Barbieri D, de Groot F, de Bruijn JD and Yuan H: Modulating Bone Regeneration in Rabbit Condyle Defects with Three Surface-Structured Tricalcium Phosphate Ceramics. ACS Biomater Sci Eng. 4:3347–3355. 2018.PubMed/NCBI View Article : Google Scholar

16 

Wang XT, Zhou CL, Yan JL, Yan X, Xie HX and Sun CL: The fate of donor osteocytes in fine particulate bone powders during repair of bone defects in experimental rats. Acta Histochem. 114:192–198. 2012.PubMed/NCBI View Article : Google Scholar

17 

Xie H, Ji Y, Tian Q, Wang X, Zhang N, Zhang Y, Xu J, Wang N and Yan J: Autogenous bone particle/titanium fiber composites for bone regeneration in a rabbit radius critical-size defect model. Connect Tissue Res. 58:553–561. 2017.PubMed/NCBI View Article : Google Scholar

18 

Sun YX, Sun CL, Tian Y, Xu WX, Zhou CL, Xi CY, Yan JL and Wang XT: A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model. Acta Histochem. 116:1015–1021. 2014.PubMed/NCBI View Article : Google Scholar

19 

Sun C, Tian Y, Xu W, Zhou C, Xie H and Wang X: Development and performance analysis of Si-CaP/fine particulate bone powder combined grafts for bone regeneration. Biomed Eng Online. 14(47)2015.PubMed/NCBI View Article : Google Scholar

20 

Graham N and Qian BZ: Mesenchymal Stromal Cells: Emerging Roles in Bone Metastasis. Int J Mol Sci. 19(1121)2018.PubMed/NCBI View Article : Google Scholar

21 

Tajima S, Tobita M, Orbay H, Hyakusoku H and Mizuno H: Direct and indirect effects of a combination of adipose-derived stem cells and platelet-rich plasma on bone regeneration. Tissue Eng Part A. 21:895–905. 2015.PubMed/NCBI View Article : Google Scholar

22 

Tian Y, Cui L-H, Xiang S-Y, Xu WX, Chen DC, Fu R, Zhou CL, Liu XQ, Wang YF and Wang XT: Osteoblast-oriented differentiation of BMSCs by co-culturing with composite scaffolds constructed using silicon-substituted calcium phosphate, autogenous fine particulate bone powder and alginate in vitro. Oncotarget. 8:88308–88319. 2017.PubMed/NCBI View Article : Google Scholar

23 

Zhang D, Huang D, Huang Y, Liu Y, Lin B, Yu C, Mou Y, Wu W, Zhang H and Lin H: Efficacy of combined therapy of periosteum and bone allograft in a critical-sized defect model in New Zealand white rabbits. Med Sci Monit. 20:2394–2403. 2014.PubMed/NCBI View Article : Google Scholar

24 

Liu F, Chen K, Hou L, Li K, Wang D, Zhang B and Wang X: Determining the critical size of a rabbit rib segmental bone defect model. Regen Biomater. 3:323–328. 2016.PubMed/NCBI View Article : Google Scholar

25 

Błaszczyk B, Kaspera W, Ficek K, Kajor M, Binkowski M, Stodolak-Zych E, Grajoszek A, Stojko J, Bursig H and Ładziński P: Effects of Polylactide Copolymer Implants and Platelet-Rich Plasma on Bone Regeneration within a Large Calvarial Defect in Sheep. BioMed Res Int. 2018(4120471)2018.PubMed/NCBI View Article : Google Scholar

26 

Qi Y, Niu L, Zhao T, Shi Z, Di T, Feng G, Li J and Huang Z: Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: A novel strategy to promote bone regeneration. Stem Cell Res Ther. 6(256)2015.PubMed/NCBI View Article : Google Scholar

27 

Guerra I, Morais Branco F, Vasconcelos M, Afonso A, Figueiral H and Zita R: Evaluation of implant osseointegration with different regeneration techniques in the treatment of bone defects around implants: An experimental study in a rabbit model. Clin Oral Implants Res. 22:314–322. 2011.PubMed/NCBI View Article : Google Scholar

28 

Broggini N, Hofstetter W, Hunziker E, Bosshardt DD, Bornstein MM, Seto I, Weibrich G and Buser D: The influence of PRP on early bone formation in membrane protected defects. A histological and histomorphometric study in the rabbit calvaria. Clin Implant Dent Relat Res. 13:1–12. 2011.PubMed/NCBI View Article : Google Scholar

29 

Fernandes G and Yang S: Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering. Bone Res. 4(16036)2016.PubMed/NCBI View Article : Google Scholar

30 

Niemeyer P, Fechner K, Milz S, Richter W, Suedkamp NP, Mehlhorn AT, Pearce S and Kasten P: Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma. Biomaterials. 31:3572–3579. 2010.PubMed/NCBI View Article : Google Scholar

31 

Lai F, Kakudo N, Morimoto N, Taketani S, Hara T, Ogawa T and Kusumoto K: Platelet-rich plasma enhances the proliferation of human adipose stem cells through multiple signaling pathways. Stem Cell Res Ther. 9(107)2018.PubMed/NCBI View Article : Google Scholar

32 

Eda T, Takahashi K, Kanao S, et al: Comparison study between plasma rich in growth factors and platelet-rich plasma for osteoconduction in rat calvaria. J Oral Maxillofac Surg. 29:563–569. 2017.

33 

Wang D, Weng Y, Guo S, Zhang Y, Zhou T, Zhang M, Wang L and Ma J: Platelet-rich plasma inhibits RANKL-induced osteoclast differentiation through activation of Wnt pathway during bone remodeling. Int J Mol Med. 41:729–738. 2018.PubMed/NCBI View Article : Google Scholar

34 

Qian Y, Han Q, Chen W, Song J, Zhao X, Ouyang Y, Yuan W and Fan C: Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration. Front Chem. 5(89)2017.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Xie H, Cao L, Ye L, Du J, Shan G, Hu J, Jiang C and Song W: Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits. Exp Ther Med 20: 279, 2020.
APA
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J. ... Song, W. (2020). Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits. Experimental and Therapeutic Medicine, 20, 279. https://doi.org/10.3892/etm.2020.9409
MLA
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J., Jiang, C., Song, W."Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits". Experimental and Therapeutic Medicine 20.6 (2020): 279.
Chicago
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J., Jiang, C., Song, W."Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits". Experimental and Therapeutic Medicine 20, no. 6 (2020): 279. https://doi.org/10.3892/etm.2020.9409
Copy and paste a formatted citation
x
Spandidos Publications style
Xie H, Cao L, Ye L, Du J, Shan G, Hu J, Jiang C and Song W: Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits. Exp Ther Med 20: 279, 2020.
APA
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J. ... Song, W. (2020). Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits. Experimental and Therapeutic Medicine, 20, 279. https://doi.org/10.3892/etm.2020.9409
MLA
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J., Jiang, C., Song, W."Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits". Experimental and Therapeutic Medicine 20.6 (2020): 279.
Chicago
Xie, H., Cao, L., Ye, L., Du, J., Shan, G., Hu, J., Jiang, C., Song, W."Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits". Experimental and Therapeutic Medicine 20, no. 6 (2020): 279. https://doi.org/10.3892/etm.2020.9409
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