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miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1

  • Authors:
    • Yuxia Li
    • Jun Zhuang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Wuhan Children's Hospital, Wuhan Maternal and Child Healthcare Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430010, P.R. China, Department of Obstetrics, Lianshui County People's Hospital, Huai'an, Jiangsu 223400, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
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    Published online on: November 2, 2020
       https://doi.org/10.3892/etm.2020.9434
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Abstract

Recent studies have demonstrated that microRNAs (miRs) serve a crucial role during the development of gestational diabetes mellitus (GDM). However, the mechanisms underlying miR‑345‑3p and its protective role during GDM have not been previously reported. The present study investigated miR‑345‑3p expression and function in vitro, and the possible molecular mechanisms underlying GDM. Compared with healthy pregnant women, miR‑345‑3p was downregulated in the placental tissue and peripheral blood of patients with GDM. Further investigation revealed that BCL2‑antagonist/killer 1 (BAK1) was a predicted target gene of miR‑345‑3p, and the expression of BAK1 was significantly increased in patients with GDM compared with healthy pregnant women. In vitro analysis revealed that miR‑345‑3p mimic significantly increased cell viability, migration and invasion, inhibited apoptosis, upregulated Bcl‑2 and matrix metallopeptidase 9 expression, and decreased Bax expression compared with the control group. Furthermore, miR‑245‑3p mimic‑induced alterations were reversed by BAK1 overexpression. The results suggested that miR‑345‑3p overexpression exhibited a protective role in patients with GDM by inhibiting HTR8‑/SVneo cell apoptosis, and promoting cell proliferation and migration via targeting BAK1. The use of miR‑345‑3p for the diagnosis of GDM requires further investigation.
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1 

Metzger BE and Coustan DR: Summary and recommendations of the Fourth International Workshop-Conference on Gestational Diabetes Mellitus. The Organizing Committee. Diabetes Care. 21 (Suppl 2):B161–B167. 1998.PubMed/NCBI

2 

Chiefari E, Arcidiacono B, Foti D and Brunetti A: Gestational diabetes mellitus: An updated overview. J Endocrinol Invest. 40:899–909. 2017.PubMed/NCBI View Article : Google Scholar

3 

Zhu Y and Zhang C: Prevalence of gestational diabetes and risk of progression to type 2 diabetes: A global perspective. Curr Diab Rep. 16(7)2016.PubMed/NCBI View Article : Google Scholar

4 

Yuen L and Wong VW: Gestational diabetes mellitus: Challenges for different ethnic groups. World J Diabetes. 6:1024–1032. 2015.PubMed/NCBI View Article : Google Scholar

5 

Egan AM, Bogdanet D, Griffin TP, Kgosidialwa O, Cervar-Zivkovic M, Dempsey E, Allotey J, Alvarado F, Clarson C, Cooray SD, et al: A core outcome set for studies of gestational diabetes mellitus prevention and treatment. Diabetologia: Mar 20, 2020 (Epub ahead of print).

6 

Tryggestad JB, Vishwanath A, Jiang S, Mallappa A, Teague AM, Takahashi Y, Thompson DM and Chernausek SD: Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA. Clin Sci (Lond). 21:1955–1967. 2016.PubMed/NCBI View Article : Google Scholar

7 

Gabbay-Benziv R and Baschat AA: Gestational diabetes as one of the ‘great obstetrical syndromes’-The maternal, placental, and fetal dialog. Best Pract Res Clin Obstet Gynaecol. 29:150–155. 2015.PubMed/NCBI View Article : Google Scholar

8 

Winship A, Sorby K, Correia J, Rainczuk A, Yap J and Dimitriadis E: Interleukin-11 up-regulates endoplasmic reticulum stress induced target, PDIA4 in human first trimester placenta and in vivo in mice. Placenta. 53:92–100. 2017.PubMed/NCBI View Article : Google Scholar

9 

Antoniotti GS, Coughlan M, Salamonsen LA and Evans J: Obesity associated advanced glycation end products within the human uterine cavity adversely impact endometrial function and embryo implantation competence. Hum Reprod. 33:654–665. 2018.PubMed/NCBI View Article : Google Scholar

10 

Graham CH, Hawley TS, Hawley RG, MacDougall JR, Kerbel RS, Khoo N and Lala PK: Establishment and characterization of first trimester human trophoblast cells with extended lifespan. Exp Cell Res. 206:204–211. 1993.PubMed/NCBI View Article : Google Scholar

11 

Monfared YK, Ghadimi F, Foroughi F, Honardoost M, Hashemipour S, Sefidi F and Sarookhani MR: Determination and comparison miR135a in the serum between women with GDM, non-pregnant type 2 diabetes, healthy pregnant and control group. Middle East J Fam Med. 2:193–197. 2018.

12 

Eminaga O, Fries J, Woetzel F, Alakus H, Warnecke-Eberz U and Heidenreich A: MP66-12 the expression profiles of miR-210, miR-375, miR-378, miR-345, miR-143 miR-183 and miR-98 in the progression of prostate cancer from high-grade prostatic intraepithelial neoplasia to metastatic diseases. J Urology. 4(e877)2016.

13 

Mou T, Xie F, Zhong P, Hua H, Lai L, Yang Q and Wang J: MiR-345-5p functions as a tumor suppressor in pancreatic cancer by directly targeting CCL8. Biomed Pharmacother. 111:891–900. 2019.PubMed/NCBI View Article : Google Scholar

14 

Danese E, Minicozzi AM, Benati M, Paviati E, Lima-Oliveira G, Gusella M, Pasini F, Salvagno GL, Montagnana M and Lippi G: Reference miRNAs for colorectal cancer: Analysis and verification of current data. Sci Rep. 7(8413)2017.PubMed/NCBI View Article : Google Scholar

15 

Ying X, Zhang W, Fang M, Zhang W, Wang C and Han L: miR-345-5p regulates proliferation, cell cycle, and apoptosis of acute myeloid leukemia cells by targeting AKT2. J Cell Biochem. 2:1620–1629. 2019.PubMed/NCBI View Article : Google Scholar

16 

Wang SY, Shiboski S, Belair CD, Cooperberg MR, Simko JP, Stoppler H, Cowan J, Carroll PR and Blelloch R: MiR-19, miR-345, miR-519c-5p serum levels predict adverse pathology in prostate cancer patients eligible for active surveillance. PLoS One. 6(e98597)2014.PubMed/NCBI View Article : Google Scholar

17 

Tinay I, Tan M, Gui B, Werner L, Kibel AS and Jia L: Functional roles and potential clinical application of miRNA-345-5p in prostate cancer. Prostate. 78:927–937. 2018.PubMed/NCBI View Article : Google Scholar

18 

Srivastava SK, Bhardwaj A, Arora S, Tyagi N, Singh S, Andrews J, McClellan S, Wang B and Singh AP: MicroRNA-345 induces apoptosis in pancreatic cancer cells through potentiation of caspase-dependent and -independent pathways. Brit J Cancer. 113:660–668. 2015.PubMed/NCBI View Article : Google Scholar

19 

Tinay I, Gui B, Werner L, Rafiei S, Gelpi-Hammerschmidt F, Kibel A and Jia L: 24-Upregulation of microRNAs miR-9, miR-330-3p and miR-345 in prostate cancer. Eur Urol Suppl. 15:e1286–e1287. 2016.

20 

Siengdee P, Trakooljul N, Murani E, Schwerin M, Wimmers K and Ponsuksili S: MicroRNAs regulate cellular ATP levels by targeting mitochondrial energy metabolism genes during C2C12 myoblast differentiation. PLoS One. 10(e0127850)2015.PubMed/NCBI View Article : Google Scholar

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

22 

Uren RT, O'Hely M, Iyer S, Bartolo R, Shi MX, Brouwer JM, Alsop AE, Dewson G and Kluck RM: Disordered clusters of Bak dimers rupture mitochondria during apoptosis. Elife. 6(pii: 19944)2017.PubMed/NCBI View Article : Google Scholar

23 

Loegl J, Nussbaumer E, Cvitic S, Huppertz B, Desoye G and Hiden U: GDM alters paracrine regulation of feto-placental angiogenesis via the trophoblast. Lab Invest. 97:409–418. 2017.PubMed/NCBI View Article : Google Scholar

24 

Mohr AM and Mott JL: Overview of microRNA biology. Semin Liver Dis. 35:3–11. 2015.PubMed/NCBI View Article : Google Scholar

25 

Lu TX and Rothenberg ME: MicroRNA. J Allergy Clin Immunol. 141:1202–1207. 2018.PubMed/NCBI View Article : Google Scholar

26 

Li J, Song L, Zhou L, Wu J, Sheng C, Chen H, Liu Y, Gao S and Huang W: A MicroRNA signature in gestational diabetes mellitus associated with risk of macrosomia. Cell Physiol Bioche. 1:243–252. 2015.PubMed/NCBI View Article : Google Scholar

27 

Erener S, Marwaha A, Tan R, Panagiotopoulos C and Kieffer TJ: Profiling of circulating microRNAs in children with recent onset of type 1 diabetes. JCI Insight. 4(e89656)2017.PubMed/NCBI View Article : Google Scholar

28 

Silva VA, Polesskaya A, Sousa TA, Corrêa VM, André ND, Reis RI, Kettelhut IC, Harel-Bellan A and De Lucca FL: Expression and cellular localization of microRNA-29b and RAX, an activator of the RNA-dependent protein kinase (PKR), in the retina of streptozotocin-induced diabetic rats. Mol Vis. 17:2228–2240. 2011.PubMed/NCBI

29 

Chen HY, Zhong X, Huang XR, Meng XM, You Y, Chung AC and Lan HY: MicroRNA-29b inhibits diabetic nephropathy in db/db mice. Mol Ther. 22:842–853. 2014.PubMed/NCBI View Article : Google Scholar

30 

Knöfler M, Haider S, Saleh L, Pollheimer J, Gamage TKJB and James J: Human placenta and trophoblast development: Key molecular mechanisms and model systems. Cell Mol Life Sci. 76:3479–3496. 2019.PubMed/NCBI View Article : Google Scholar

31 

Pollheimer J, Vondra S, Baltayeva J, Beristain AG and Knöfler M: Regulation of placental extravillous trophoblasts by the maternal uterine environment. Front Immunol. 9(2597)2018.PubMed/NCBI View Article : Google Scholar

32 

Haider S, Meinhardt G, Saleh L, Kunihs V, Gamperl M, Kaindl U, Ellinger A, Burkard TR, Fiala C, Pollheimer J, et al: Self-renewing trophoblast organoids recapitulate the developmental program of the early human placenta. Stem Cell Reports. 11:537–551. 2018.PubMed/NCBI View Article : Google Scholar

33 

Wang X, Peng S, Cui K, Hou F, Ding J, Li A, Wang M and Geng L: MicroRNA-576-5p enhances the invasion ability of trophoblast cells in preeclampsia by targeting TFAP2A. Mol Genet Genomic Med. 8(e1025)2020.PubMed/NCBI View Article : Google Scholar

34 

Baines KJ and Renaud SJ: Transcription factors that regulate trophoblast development and function. Prog Mol Biol Transl Sci. 145:39–88. 2017.PubMed/NCBI View Article : Google Scholar

35 

Aplin JD: Developmental cell biology of human villous trophoblast: Current research problems. Int J Dev Biol. 54:323–329. 2010.PubMed/NCBI View Article : Google Scholar

36 

Meekins JW, Pijnenborg R, Hanssens M, McFadyen IR and van Asshe A: A study of placental bed spiral arteries and trophoblast invasion in normal and severe pre-eclamptic pregnancies. Brit J Obstet Gynaec. 8:669–674. 1994.PubMed/NCBI View Article : Google Scholar

37 

Mayama R, Izawa T, Sakai K, Suciu N and Iwashita M: Improvement of insulin sensitivity promotes extravillous trophoblast cell migration stimulated by insulin-like growth factor-I. Endocr J. 60:359–368. 2013.PubMed/NCBI View Article : Google Scholar

38 

Aires MB and Anne Carolline Veríssimo dos Santos: Effects of maternal diabetes on trophoblast cells. World J Diabetes. 6:338–344. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li Y and Zhuang J: miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Exp Ther Med 21: 2, 2021.
APA
Li, Y., & Zhuang, J. (2021). miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Experimental and Therapeutic Medicine, 21, 2. https://doi.org/10.3892/etm.2020.9434
MLA
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21.1 (2021): 2.
Chicago
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21, no. 1 (2021): 2. https://doi.org/10.3892/etm.2020.9434
Copy and paste a formatted citation
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Spandidos Publications style
Li Y and Zhuang J: miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Exp Ther Med 21: 2, 2021.
APA
Li, Y., & Zhuang, J. (2021). miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Experimental and Therapeutic Medicine, 21, 2. https://doi.org/10.3892/etm.2020.9434
MLA
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21.1 (2021): 2.
Chicago
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21, no. 1 (2021): 2. https://doi.org/10.3892/etm.2020.9434
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