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Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas

  • Authors:
    • Chen Liang
    • Jian Shangguan
    • Ling Yang
    • Shiwen Guo
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Aeromedical Physical Examination, Xi'an Civil Aviation Hospital, Xi'an, Shaanxi 710082, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 22
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    Published online on: November 6, 2020
       https://doi.org/10.3892/etm.2020.9454
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Abstract

Vasculogenic mimicry (VM) contributes to the resistance of anti‑angiogenic therapies in glioma. Certain genes, including MMP‑2 and VEGF may be associated with the development of VM. Astrocyte elevated gene‑1 (AEG‑1) is considered to be an oncogene that promotes autophagy, invasion, metastasis, angiogenesis and drug resistance; however, the association between AEG‑1 and VM formation is still unknown. The present study investigated the effects of AEG‑1 downregulation on VM formation in the U87 glioma cell line in vitro and in xenograft models of glioma, and the potential underlying mechanisms of action. In the present study, U87 glioma cells were infected with the AEG‑1 short hairpin RNA lentivirus. A Matrigel‑based tube formation assay was performed to evaluate VM formation in vitro. Reverse transcription‑quantitative PCR and western blot analysis were conducted to investigate the mRNA and protein expression levels of MMP‑2 and VEGF. Glioma xenograft models were generated through the intracerebral implantation of U87 glioma cells into nude rats; CD34/Periodic Acid‑Schiff double‑staining was performed to detect VM channels in vivo. Following AEG‑1 downregulation in U87 cells, the development of VM was significantly decreased in vitro and in vivo. In addition, the expression levels of MMP‑2 and VEGF in glioma cells were decreased compared with the control group. These results suggested that downregulation of AEG‑1 expression could significantly inhibit the development of VM in gliomas, both in vitro and in vivo, and may be partially related to the regulation of VEGF and MMP‑2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Liang C, Shangguan J, Yang L and Guo S: Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med 21: 22, 2021.
APA
Liang, C., Shangguan, J., Yang, L., & Guo, S. (2021). Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas. Experimental and Therapeutic Medicine, 21, 22. https://doi.org/10.3892/etm.2020.9454
MLA
Liang, C., Shangguan, J., Yang, L., Guo, S."Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas". Experimental and Therapeutic Medicine 21.1 (2021): 22.
Chicago
Liang, C., Shangguan, J., Yang, L., Guo, S."Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas". Experimental and Therapeutic Medicine 21, no. 1 (2021): 22. https://doi.org/10.3892/etm.2020.9454
Copy and paste a formatted citation
x
Spandidos Publications style
Liang C, Shangguan J, Yang L and Guo S: Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med 21: 22, 2021.
APA
Liang, C., Shangguan, J., Yang, L., & Guo, S. (2021). Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas. Experimental and Therapeutic Medicine, 21, 22. https://doi.org/10.3892/etm.2020.9454
MLA
Liang, C., Shangguan, J., Yang, L., Guo, S."Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas". Experimental and Therapeutic Medicine 21.1 (2021): 22.
Chicago
Liang, C., Shangguan, J., Yang, L., Guo, S."Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas". Experimental and Therapeutic Medicine 21, no. 1 (2021): 22. https://doi.org/10.3892/etm.2020.9454
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