Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
January-2021 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2021 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway

  • Authors:
    • Guangzhao Song
    • Liyan Li
    • Ying Yang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First People's Hospital of Jinan, Jinan, Shandong 250011, P.R. China, Department of Medicine, Licheng District Maternal and Child Healthcare and Family Planning Service Center, Jinan, Shandong 250100, P.R. China
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 29
    |
    Published online on: November 10, 2020
       https://doi.org/10.3892/etm.2020.9461
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the current study was to determine the expression of microRNA (miRNA/miR)‑329‑3p in patients with type 2 diabetes mellitus (T2DM) and to investigate the effect of miR‑329‑3p on vascular endothelial cell function under high‑glucose conditions. A total of 33 healthy individuals and 31 patients with T2DM were enrolled in the present study. Peripheral blood was collected from all participants. Human umbilical vein endothelial cells (HUVECs) were transfected with a miR‑329‑3p mimic or miR‑329‑3p inhibitor. Following treatment with 25 mmol/l glucose, a Cell Counting Kit‑8 assay and flow cytometry analysis were used to assess cell viability and apoptosis levels, respectively. A dual luciferase reporter assay, western blot analysis and reverse transcription‑quantitative PCR were used to assess molecular mechanism of miR‑329‑3p in HUVECs. The results revealed that plasma miR‑329‑3p expression was decreased patients with T2DM compared with healthy controls, and in HUVECs treated with high glucose concentrations. In addition, miR‑329‑3p reduced high glucose‑induced damage to HUVEC cells. miR‑329‑3p directly bound to toll like receptor (TLR)‑4 and regulated its expression at the transcriptional and post‑transcriptional levels. miR‑329‑3p was also demonstrated to be involved in the regulation of the TLR4/tumor necrosis factor receptor associated factor 6 (TRAF6)/nuclear factor (NF)‑κB signaling pathway and the nuclear translocation of NF‑κB under a high glucose environment. In conclusion, the results indicated that miR‑329‑3p may protect endothelial cells from high glucose‑induced apoptosis via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway. The present study also demonstrated that miR‑329‑3p expression in the plasma of patients with T2DM was reduced, suggesting that upregulation of miR‑329‑3p may alleviate high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Hurlow JJ, Humphreys GJ, Bowling FL and McBain AJ: Diabetic foot infection: A critical complication. Int Wound J. 15:814–821. 2018.PubMed/NCBI View Article : Google Scholar

2 

Knapp M, Tu X and Wu R: Vascular endothelial dysfunction, a major mediator in diabetic cardiomyopathy. Acta Pharmacol Sin. 40:1–8. 2019.PubMed/NCBI View Article : Google Scholar

3 

Eelen G, de Zeeuw P, Simons M and Carmeliet P: Endothelial cell metabolism in normal and diseased vasculature. Circ Res. 116:1231–1244. 2015.PubMed/NCBI View Article : Google Scholar

4 

Nakagami H, Kaneda Y, Ogihara T and Morishita R: Endothelial dysfunction in hyperglycemia as a trigger of atherosclerosis. Curr Diabet Rev. 1:59–63. 2005.PubMed/NCBI View Article : Google Scholar

5 

Mirra P, Raciti GA, Nigro C, Fiory F, D'Esposito V, Formisano P, Beguinot F and Miele C: Circulating miRNAs as intercellular messengers, potential biomarkers and therapeutic targets for Type 2 diabetes. Epigenomics. 7:653–667. 2015.PubMed/NCBI View Article : Google Scholar

6 

Hou LJ, Han JJ and Liu Y: Up-regulation of microRNA-503 by high glucose reduces the migration and proliferation but promotes the apoptosis of human umbilical vein endothelial cells by inhibiting the expression of insulin-like growth factor-1 receptor. Eur Rev Med Pharmacol Sci. 22:3515–3523. 2018.PubMed/NCBI View Article : Google Scholar

7 

Hui Y and Yin Y: MicroRNA-145 attenuates high glucose-induced oxidative stress and inflammation in retinal endothelial cells through regulating TLR4/NF-kappaB signaling. Life Sci. 207:212–218. 2018.PubMed/NCBI View Article : Google Scholar

8 

Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC and Cavaille J: A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain. Genome Res. 14:1741–1748. 2004.PubMed/NCBI View Article : Google Scholar

9 

Benetatos L, Hatzimichael E, Londin E, Vartholomatos G, Loher P, Rigoutsos I and Briasoulis E: The microRNAs within the DLK1-DIO3 genomic region: Involvement in disease pathogenesis. Cell Mol Life Sci. 70:795–814. 2013.PubMed/NCBI View Article : Google Scholar

10 

Welten SM, Bastiaansen AJ, de Jong RC, de Vries MR, Peters EA, Boonstra MC, Sheikh SP, La Monica N, Kandimalla ER, Quax PH and Nossent AY: Inhibition of 14q32 MicroRNAs miR-329, miR-487b, miR-494, and miR-495 increases neovascularization and blood flow recovery after ischemia. Circ Res. 115:696–708. 2014.PubMed/NCBI View Article : Google Scholar

11 

Wang P, Luo Y, Duan H, Xing S, Zhang J, Lu D, Feng J, Yang D, Song L and Yan X: MicroRNA 329 suppresses angiogenesis by targeting CD146. Mol Cell Biol. 33:3689–3699. 2013.PubMed/NCBI View Article : Google Scholar

12 

Vaure C and Liu YL: ‘A comparative review of toll-like receptor 4 expression and functionality in different animal species’. Front Immunol. 5(316)2014.PubMed/NCBI View Article : Google Scholar

13 

Hu L, Yang H, Ai M and Jiang S: Inhibition of TLR4 alleviates the inflammation and apoptosis of retinal ganglion cells in high glucose. Graefes Arch Clin Exp Ophthalmol. 255:2199–2210. 2017.PubMed/NCBI View Article : Google Scholar

14 

Ye EA and Steinle JJ: miR-146a attenuates inflammatory pathways mediated by TLR4/NF-κB and TNFα to protect primary human retinal Microvascular endothelial cells grown in high glucose. Mediators Inflamm. 2016(3958453)2016.PubMed/NCBI View Article : Google Scholar

15 

Zhang TH, Huang CM, Gao X, Wang JW, Hao LL and Ji Q: Gastrodin inhibits high glucose-induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF-κBp65 signaling pathway. Mol Med Rep. 17:7774–7780. 2018.PubMed/NCBI View Article : Google Scholar

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta c(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

17 

Wang X, Lu X, Zhang T, Wen C, Shi M, Tang X, Chen H, Peng C, Li H, Fang Y, et al: mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer. Oncotarget. 7:21441–21453. 2016.PubMed/NCBI View Article : Google Scholar

18 

Jiang W, Liu J, Xu T and Yu X: MiR-329 suppresses osteosarcoma development by downregulating Rab10. FEBS Lett. 590:2973–2981. 2016.PubMed/NCBI View Article : Google Scholar

19 

Li Z, Yu X, Wang Y, Shen J, Wu WK, Liang J and Feng F: By downregulating TIAM1 expression, microRNA-329 suppresses gastric cancer invasion and growth. Oncotarget. 6:17559–17569. 2015.PubMed/NCBI View Article : Google Scholar

20 

Xiao B, Tan L, He B, Liu Z and Xu R: MiRNA-329 targeting E2F1 inhibits cell proliferation in glioma cells. J Transl Med. 11(172)2013.PubMed/NCBI View Article : Google Scholar

21 

Gordon S: Pattern recognition receptors: Doubling up for the innate immune response. Cell. 111:927–930. 2002.PubMed/NCBI View Article : Google Scholar

22 

Devaraj S, Dasu MR, Rockwood J, Winter W, Griffen SC and Jialal I: Increased toll-like receptor (TLR) 2 and TLR4 expression in monocytes from patients with type 1 diabetes: Further evidence of a proinflammatory state. J Clin Endocrinol Metab. 93:578–583. 2008.PubMed/NCBI View Article : Google Scholar

23 

Dasu MR, Devaraj S, Park S and Jialal I: Increased toll-like receptor (TLR) activation and TLR ligands in recently diagnosed type 2 diabetic subjects. Diabetes Care. 33:861–868. 2010.PubMed/NCBI View Article : Google Scholar

24 

Garibotto G, Carta A, Picciotto D, Viazzi F and Verzola D: Toll-like receptor-4 signaling mediates inflammation and tissue injury in diabetic nephropathy. J Nephrol. 30:719–727. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Song G, Li L and Yang Y: MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway. Exp Ther Med 21: 29, 2021.
APA
Song, G., Li, L., & Yang, Y. (2021). MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 21, 29. https://doi.org/10.3892/etm.2020.9461
MLA
Song, G., Li, L., Yang, Y."MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 21.1 (2021): 29.
Chicago
Song, G., Li, L., Yang, Y."MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 21, no. 1 (2021): 29. https://doi.org/10.3892/etm.2020.9461
Copy and paste a formatted citation
x
Spandidos Publications style
Song G, Li L and Yang Y: MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway. Exp Ther Med 21: 29, 2021.
APA
Song, G., Li, L., & Yang, Y. (2021). MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 21, 29. https://doi.org/10.3892/etm.2020.9461
MLA
Song, G., Li, L., Yang, Y."MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 21.1 (2021): 29.
Chicago
Song, G., Li, L., Yang, Y."MicroRNA‑329‑3p alleviates high glucose‑induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 21, no. 1 (2021): 29. https://doi.org/10.3892/etm.2020.9461
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team