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Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms

  • Authors:
    • Yanping Cun
    • Bo Diao
    • Zhimin Zhang
    • Gang Wang
    • Jing Yu
    • Lianting Ma
    • Zhiguo Rao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, General Hospital of Central Theater Command, Wuhan, Hubei 430070, P.R. China, Department of Clinical Experiment, General Hospital of Central Theater Command, Wuhan, Hubei 430070, P.R. China, Department of Neurosurgery, General Hospital of Central Theater Command, Wuhan, Hubei 430070, P.R. China
    Copyright: © Cun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 39
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    Published online on: November 17, 2020
       https://doi.org/10.3892/etm.2020.9471
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Abstract

Stromal cell derived factor‑1 (SDF‑1) is a chemokine that plays a critical role in the homing of stem and progenitor cells, including endothelial progenitor cells (EPCs). However, little research has been undertaken to evaluate the roles of SDF‑1 in the biological functions of EPCs and related signaling pathways. The present study aimed to investigate the biological functions of EPCs in response to SDF‑1, as well as the underlying mechanisms. The effects of SDF‑1 treatment on EPC proliferation, migration and tube formation were assessed by performing MTS, Transwell and in vitro tube formation assays, respectively. The phosphorylation status of Akt and ERK was evaluated by western blotting. The present results indicated that SDF‑1 treatment enhanced EPC proliferation, migration and tube formation compared with the control group. Furthermore, SDF‑1‑induced EPC proliferation was significantly reduced following treatment with a C‑X‑C Motif Chemokine Receptor 4 antagonist (AMD3100), a PI3K inhibitor (LY294002) and the mitogen‑activated protein kinase kinase inhibitor (MEK; PD98059). SDF‑1‑induced migration and angiogenesis were significantly suppressed by the PI3K inhibitor, but not the MEK inhibitor. Moreover, SDF‑1 significantly increased the protein expression levels of phosphorylated (p)‑Akt and p‑ERK; however, SDF‑1‑induced effects on protein expression were suppressed by AMD3100, LY294002 and PD98059. Thus, SDF‑1‑induced EPC proliferation was mediated by activation of the Akt and ERK signaling pathways, whereas SDF‑1‑mediated EPC migration and tube formation only involved activation of the Akt signaling pathway.
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Spandidos Publications style
Cun Y, Diao B, Zhang Z, Wang G, Yu J, Ma L and Rao Z: Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms. Exp Ther Med 21: 39, 2021.
APA
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., & Rao, Z. (2021). Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms. Experimental and Therapeutic Medicine, 21, 39. https://doi.org/10.3892/etm.2020.9471
MLA
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., Rao, Z."Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms". Experimental and Therapeutic Medicine 21.1 (2021): 39.
Chicago
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., Rao, Z."Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms". Experimental and Therapeutic Medicine 21, no. 1 (2021): 39. https://doi.org/10.3892/etm.2020.9471
Copy and paste a formatted citation
x
Spandidos Publications style
Cun Y, Diao B, Zhang Z, Wang G, Yu J, Ma L and Rao Z: Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms. Exp Ther Med 21: 39, 2021.
APA
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., & Rao, Z. (2021). Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms. Experimental and Therapeutic Medicine, 21, 39. https://doi.org/10.3892/etm.2020.9471
MLA
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., Rao, Z."Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms". Experimental and Therapeutic Medicine 21.1 (2021): 39.
Chicago
Cun, Y., Diao, B., Zhang, Z., Wang, G., Yu, J., Ma, L., Rao, Z."Role of the stromal cell derived factor‑1 in the biological functions of endothelial progenitor cells and its underlying mechanisms". Experimental and Therapeutic Medicine 21, no. 1 (2021): 39. https://doi.org/10.3892/etm.2020.9471
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