Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2

Liang,Ying; Kang,Liying; Qi,Zihe; Gao,Xing; Quan,Huili; Lin,Huifang

doi:10.3892/etm.2020.9496

Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H₂O₂

Authors:
- Ying Liang
- Liying Kang
- Zihe Qi
- Xing Gao
- Huili Quan
- Huifang Lin
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Affiliations: Reproductive Medicine Center and Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, P.R. China, Department of Science and Technology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China, Department of Gynecology and Obstetrics, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, P.R. China
Published online on: November 19, 2020 https://doi.org/10.3892/etm.2020.9496
Article Number: 64
Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water‑soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H₂O₂‑induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit‑8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor‑α (TNF‑α) were measured. Western blotting was performed to detect the protein expression of cleaved caspase‑3 and caspase‑9. Furthermore, immunocytochemistry was used to detect the expression of TNF‑α. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H₂O₂‑induced increased MDA levels, and reversed the H₂O₂‑induced suppression of SOD and GSH activities in KGN cells (P<0.05). Treatment with Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B significantly reduced the overexpression of cleaved caspase‑3, cleaved caspase‑9 and TNF‑α compared with the H₂O₂‑treated group (P<0.05). Therefore, the present results indicated that Salvia miltiorrhiza solution and its main water‑soluble compounds, salvianic acid A and salvianolic acid B, ameliorated KGN cell damage induced by H₂O₂.

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