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Diagnostic value of CXCR3 and its ligands in spinal tuberculosis

  • Authors:
    • Xiaoqian Shang
    • Liang Wang
    • Yumei Liu
    • Xuemei Liu
    • Jie Lv
    • Xuan Zhou
    • Hao Wang
    • Shaxika Nazierhan
    • Jing Wang
    • Xiumin Ma
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Spinal Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830011, P.R. China
    Copyright: © Shang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 73
    |
    Published online on: November 25, 2020
       https://doi.org/10.3892/etm.2020.9505
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Abstract

The present study aimed to investigate whether C‑X‑C motif chemokine receptor 3 (CXCR3) and its ligands may aid in diagnosing spinal tuberculosis (ST). A total of 36 patients with ST and 20 healthy controls were enrolled in the present study. The morphology of tuberculous granuloma in spinal tissue was observed by hematoxylin and eosin staining. The presence and distribution of acid‑fast bacilli (AFB) were observed by Ziehl‑Neelsen (ZN) staining. The protein expression of Ag85B, IFN‑γ, and CXCR3 and its ligands (CXCL9 and CXCL10) were detected by immunohistochemistry. The levels of IFN‑γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST and healthy controls were detected by reverse transcription‑quantitative polymerase chain reaction and ELISA. Typical tuberculous granuloma was observed in the ST close tissue. AFB was observed by ZN staining. Positive expression of Ag85B was found in the surrounding caseous necrotic tissue of the tuberculous granuloma. IFN‑γ, CXCR3, CXCL9 and CXCL10 were expressed in the tissue surrounding the tuberculous granuloma and their expression levels were markedly higher than those in the distant tissues. The levels of IFN‑γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST were significantly higher than those in the healthy controls. Receiver operating characteristic curve analysis demonstrated that IFN‑γ, CXCR3 and CXCL10 were more reliable diagnostic markers in terms of sensitivity and specificity. IFN‑γ, CXCR3, CXCL9 and CXCL10 were highly expressed in the lesion tissue and peripheral blood samples of patients with ST, and IFN‑γ, CXCR3 and its ligands aided in diagnosing ST.
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Copy and paste a formatted citation
Spandidos Publications style
Shang X, Wang L, Liu Y, Liu X, Lv J, Zhou X, Wang H, Nazierhan S, Wang J, Ma X, Ma X, et al: Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Exp Ther Med 21: 73, 2021.
APA
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X. ... Ma, X. (2021). Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Experimental and Therapeutic Medicine, 21, 73. https://doi.org/10.3892/etm.2020.9505
MLA
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X., Wang, H., Nazierhan, S., Wang, J., Ma, X."Diagnostic value of CXCR3 and its ligands in spinal tuberculosis". Experimental and Therapeutic Medicine 21.1 (2021): 73.
Chicago
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X., Wang, H., Nazierhan, S., Wang, J., Ma, X."Diagnostic value of CXCR3 and its ligands in spinal tuberculosis". Experimental and Therapeutic Medicine 21, no. 1 (2021): 73. https://doi.org/10.3892/etm.2020.9505
Copy and paste a formatted citation
x
Spandidos Publications style
Shang X, Wang L, Liu Y, Liu X, Lv J, Zhou X, Wang H, Nazierhan S, Wang J, Ma X, Ma X, et al: Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Exp Ther Med 21: 73, 2021.
APA
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X. ... Ma, X. (2021). Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Experimental and Therapeutic Medicine, 21, 73. https://doi.org/10.3892/etm.2020.9505
MLA
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X., Wang, H., Nazierhan, S., Wang, J., Ma, X."Diagnostic value of CXCR3 and its ligands in spinal tuberculosis". Experimental and Therapeutic Medicine 21.1 (2021): 73.
Chicago
Shang, X., Wang, L., Liu, Y., Liu, X., Lv, J., Zhou, X., Wang, H., Nazierhan, S., Wang, J., Ma, X."Diagnostic value of CXCR3 and its ligands in spinal tuberculosis". Experimental and Therapeutic Medicine 21, no. 1 (2021): 73. https://doi.org/10.3892/etm.2020.9505
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