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Dendritic cell‑CD4+ T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients

  • Authors:
    • Luminita Daniela Cernescu
    • Laura Haidar
    • Carmen Panaitescu
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    Affiliations: Discipline of Dermatovenerology, Department XIV Microbiology, ‘Victor Babes’ University of Medicine and Pharmacy Timisoara, Timisoara RO‑300041, Romania, Discipline of Physiology, Department III Functional Sciences, ‘Victor Babes’ University of Medicine and Pharmacy Timisoara, Timisoara RO‑300041, Romania
    Copyright: © Cernescu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 95
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    Published online on: November 26, 2020
       https://doi.org/10.3892/etm.2020.9527
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Abstract

Allergic asthma is a chronic airway inflammatory disorder triggered by inhalant allergens. Interleukin (IL)‑4 and IL‑13 play a main role in the generation of T helper cell type 2 (Th2) immune response, induction of immunoglobulin E (IgE) synthesis and persistence of airway inflammation. The aim of the present study was to investigate the influence of Dermatophagoides pteronyssinus allergen Der p 1, the major allergen of house dust mite, on the synthesis of IL‑4 and IL‑13 by monocyte‑derived dendritic cells (DCs) and naive CD4+ T cells cocultured with DCs, as well as their role in the production of serum IgE, in house dust mite (HDM) allergic patients. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood of patients allergic to HDM and healthy donors and incubated with granulocyte‑macrophage colony‑stimulating factor (GM‑CSF) and IL‑4 to generate immature DCs. The obtained cells were stimulated for 24 h with Der p 1 to induce DC maturation, washed, and afterwards cocultured for 24 h with autologous naive CD4+ T cells. Culture supernatants were harvested for IL‑4, IL‑13 and IFN‑γ level measurements. DCs stimulation with Der p 1 induced higher synthesis of IL‑4 and IL‑13 in HDM allergic patients, compared to healthy donors. The allergic group showed significant correlation between IL‑13 production by Der p 1‑pulsed DCs, and total serum IgE and IL‑4 production of the same cells and Der p‑specific IgE. To conclude, IL‑4 and IL‑13 are critically related to the regulation of serum IgE production in patients with allergic asthma. The relevance of these two cytokines in the pathophysiology of Th2 asthma endotype makes them an appropriate target in its management.
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Spandidos Publications style
Cernescu LD, Haidar L and Panaitescu C: Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients. Exp Ther Med 21: 95, 2021.
APA
Cernescu, L.D., Haidar, L., & Panaitescu, C. (2021). Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients. Experimental and Therapeutic Medicine, 21, 95. https://doi.org/10.3892/etm.2020.9527
MLA
Cernescu, L. D., Haidar, L., Panaitescu, C."Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients". Experimental and Therapeutic Medicine 21.1 (2021): 95.
Chicago
Cernescu, L. D., Haidar, L., Panaitescu, C."Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients". Experimental and Therapeutic Medicine 21, no. 1 (2021): 95. https://doi.org/10.3892/etm.2020.9527
Copy and paste a formatted citation
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Spandidos Publications style
Cernescu LD, Haidar L and Panaitescu C: Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients. Exp Ther Med 21: 95, 2021.
APA
Cernescu, L.D., Haidar, L., & Panaitescu, C. (2021). Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients. Experimental and Therapeutic Medicine, 21, 95. https://doi.org/10.3892/etm.2020.9527
MLA
Cernescu, L. D., Haidar, L., Panaitescu, C."Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients". Experimental and Therapeutic Medicine 21.1 (2021): 95.
Chicago
Cernescu, L. D., Haidar, L., Panaitescu, C."Dendritic cell‑CD4<sup>+</sup> T cell interaction: The differential role of IL‑4/IL‑13 in serum IgE levels in house dust mite allergic patients". Experimental and Therapeutic Medicine 21, no. 1 (2021): 95. https://doi.org/10.3892/etm.2020.9527
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