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Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells

  • Authors:
    • Andrada Iftode
    • George Andrei Drăghici
    • Ioana Macașoi
    • Iasmina  Marcovici
    • Dorina E. Coricovac
    • Razvan Dragoi
    • Alina Tischer
    • Leda Kovatsi
    • Aristidis M. Tsatsakis
    • Octavian Cretu
    • Cristina Dehelean
  • View Affiliations / Copyright

    Affiliations: Department of Toxicology and Drug Industry, Faculty of Pharmacy, ‘Victor Babes’ University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Balneology, Rehabilitation and Rheumatology, ‘Victor Babes’ University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Surgery I, Faculty of Medicine, ‘Victor Babes’ University of Medicine and Pharmacy, 300041 Timisoara, Romania, Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece, Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece
    Copyright: © Iftode et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 100
    |
    Published online on: November 26, 2020
       https://doi.org/10.3892/etm.2020.9532
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Abstract

Recent scientific evidence suggests a link between epigenetic changes (DNA methylation) and tumorigenesis. Moreover, a potential carcinogenic mechanism of cadmium was associated with changes in DNA methylation. In this study we investigated the impact of CdCl2 and CuSO4 aqueous solutions on DNA methylation in HT‑29 cells by quantifying DNA methyltransferase (DNMT1, DNMT3A and DNMT3B) mRNA expression. Furthermore, we also studied the cytotoxic and anti‑migratory potential of these substances. The results showed a dose‑dependent decrease of viable cell percentage following 24 h of exposure (at concentrations of 0.05; 0.2; 1; 10 and 100 µg/ml), and an inhibitory effect on HT‑29 cell migration capacity. In addition, RT‑qPCR results showed that cadmium acts as a hypomethylating agent by suppressing DNMT expression, whereas copper acts as a hypermethylating compound by increasing DNMT expression. These findings suggest a cytotoxic potential of both cadmium and copper on HT‑29 cells and their capacity to induce epigenetic changes.
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Iftode A, Drăghici GA, Macașoi I, Marcovici I, Coricovac DE, Dragoi R, Tischer A, Kovatsi L, Tsatsakis AM, Cretu O, Cretu O, et al: Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells. Exp Ther Med 21: 100, 2021.
APA
Iftode, A., Drăghici, G.A., Macașoi, I., Marcovici, I., Coricovac, D.E., Dragoi, R. ... Dehelean, C. (2021). Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells. Experimental and Therapeutic Medicine, 21, 100. https://doi.org/10.3892/etm.2020.9532
MLA
Iftode, A., Drăghici, G. A., Macașoi, I., Marcovici, I., Coricovac, D. E., Dragoi, R., Tischer, A., Kovatsi, L., Tsatsakis, A. M., Cretu, O., Dehelean, C."Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells". Experimental and Therapeutic Medicine 21.1 (2021): 100.
Chicago
Iftode, A., Drăghici, G. A., Macașoi, I., Marcovici, I., Coricovac, D. E., Dragoi, R., Tischer, A., Kovatsi, L., Tsatsakis, A. M., Cretu, O., Dehelean, C."Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells". Experimental and Therapeutic Medicine 21, no. 1 (2021): 100. https://doi.org/10.3892/etm.2020.9532
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Spandidos Publications style
Iftode A, Drăghici GA, Macașoi I, Marcovici I, Coricovac DE, Dragoi R, Tischer A, Kovatsi L, Tsatsakis AM, Cretu O, Cretu O, et al: Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells. Exp Ther Med 21: 100, 2021.
APA
Iftode, A., Drăghici, G.A., Macașoi, I., Marcovici, I., Coricovac, D.E., Dragoi, R. ... Dehelean, C. (2021). Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells. Experimental and Therapeutic Medicine, 21, 100. https://doi.org/10.3892/etm.2020.9532
MLA
Iftode, A., Drăghici, G. A., Macașoi, I., Marcovici, I., Coricovac, D. E., Dragoi, R., Tischer, A., Kovatsi, L., Tsatsakis, A. M., Cretu, O., Dehelean, C."Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells". Experimental and Therapeutic Medicine 21.1 (2021): 100.
Chicago
Iftode, A., Drăghici, G. A., Macașoi, I., Marcovici, I., Coricovac, D. E., Dragoi, R., Tischer, A., Kovatsi, L., Tsatsakis, A. M., Cretu, O., Dehelean, C."Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells". Experimental and Therapeutic Medicine 21, no. 1 (2021): 100. https://doi.org/10.3892/etm.2020.9532
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