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Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts

  • Authors:
    • Fang Xie
    • Li Teng
    • Jiajie Xu
    • Jianjian Lu
    • Chao Zhang
    • Liya Yang
    • Xiaoyang Ma
    • Minghao Zhao
  • View Affiliations / Copyright

    Affiliations: Cranio‑Maxillo‑Facial Surgery Department 2, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 139
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    Published online on: December 14, 2020
       https://doi.org/10.3892/etm.2020.9571
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Abstract

Pathological scars occur during skin wound healing, and the use of adipose‑derived stem cells (ADSCs) is one of the various treatments. The present study aimed to investigate the in vitro effects of ADSCs on the biological properties of hypertrophic scar fibroblasts (HSFs) and keloid fibroblasts (KFs), such as proliferation, migration, and the synthesis of extracellular matrix proteins. Transwell chambers were used to establish a co‑culture system of ADSCs with normal skin fibroblasts (NFs), HSFs or KFs. The effect of ADSCs on the proliferation of fibroblasts was evaluated by CCK8 measurement, while the migration ability of fibroblasts was assessed using cell scratch assay. The expression of extracellular matrix proteins was measured by immunoblotting. Co‑culture of NFs with ADSCs did not affect cell proliferation and migration, nor the expression of extracellular matrix proteins [collagen‑I, collagen‑III, fibronectin (FN) and α‑smooth muscle actin (α‑SMA)] in NFs. However, as with the inhibitor SB431542, ADSCs significantly inhibited cell proliferation and migration and the expression of extracellular matrix proteins (collagen‑I, collagen‑III, FN and α‑SMA), but also suppressed the protein expression of transforming growth factor β1 (TGF‑β1), phosphorylated (p‑) mothers against decapentaplegic homolog (Smad) 2, p‑Smad3 and Smad7 in HSFs and KFs. The results show that ADSCs inhibited cell proliferation and migration and the expression of extracellular matrix proteins in HSCs and KFs in vitro, possibly through inhibition of the TGF‑β1/Smad pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xie F, Teng L, Xu J, Lu J, Zhang C, Yang L, Ma X and Zhao M: Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts. Exp Ther Med 21: 139, 2021.
APA
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L. ... Zhao, M. (2021). Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts. Experimental and Therapeutic Medicine, 21, 139. https://doi.org/10.3892/etm.2020.9571
MLA
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L., Ma, X., Zhao, M."Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts". Experimental and Therapeutic Medicine 21.2 (2021): 139.
Chicago
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L., Ma, X., Zhao, M."Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts". Experimental and Therapeutic Medicine 21, no. 2 (2021): 139. https://doi.org/10.3892/etm.2020.9571
Copy and paste a formatted citation
x
Spandidos Publications style
Xie F, Teng L, Xu J, Lu J, Zhang C, Yang L, Ma X and Zhao M: Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts. Exp Ther Med 21: 139, 2021.
APA
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L. ... Zhao, M. (2021). Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts. Experimental and Therapeutic Medicine, 21, 139. https://doi.org/10.3892/etm.2020.9571
MLA
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L., Ma, X., Zhao, M."Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts". Experimental and Therapeutic Medicine 21.2 (2021): 139.
Chicago
Xie, F., Teng, L., Xu, J., Lu, J., Zhang, C., Yang, L., Ma, X., Zhao, M."Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts". Experimental and Therapeutic Medicine 21, no. 2 (2021): 139. https://doi.org/10.3892/etm.2020.9571
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