Open Access

Role of miRNAs and lncRNAs in dexamethasone‑induced myotube atrophy in vitro

  • Authors:
    • Yang Li
    • Huacai Shi
    • Rui Chen
    • Shanyao Zhou
    • Si Lei
    • Yanling She
  • View Affiliations

  • Published online on: December 16, 2020     https://doi.org/10.3892/etm.2020.9577
  • Article Number: 146
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Skeletal muscle atrophy is a well‑known adverse effect of long‑term glucocorticoid (GC) therapy. MicroRNAs (miRNAs or miRs) and long non‑coding RNAs (lncRNAs) are important regulators in a number of physiological and pathological processes. However, the role of miRNAs and lncRNAs in the regulation of GC‑treated muscle atrophy remains poorly understood. In the current study, muscular atrophy was induced and the results indicated that C2C12 myotubes were thinner than normal, while the expression of muscle ring finger protein 1 and Atrogin‑1 was increased. The expression of nine miRNAs and seven lncRNAs associated with proliferation and differentiation were analyzed in a dexamethasone (DEX)‑induced muscle atrophy cell model. In addition, the mRNA expression of the downstream targets of lncRNAs that were differentially expressed between DEX‑treated and control cells were determined. The results indicated that the expression of miR‑133a, miR‑133b, miR‑206 and five lncRNAs (increased Atrolnc‑1, Dum, MAR1, linc‑MD1 and decreased Myolinc) were significantly different between the DEX and the control group. Furthermore, the relative mRNA expression of Wnt5a and MyoD was significantly different between the two groups. The results of the current study indicated that some important miRNAs and lncRNAs are associated with DEX‑induced muscle atrophy and have the potential to be further developed as a diagnostic tool for this condition.
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February-2021
Volume 21 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Li Y, Shi H, Chen R, Zhou S, Lei S and She Y: Role of miRNAs and lncRNAs in dexamethasone‑induced myotube atrophy <em>in vitro</em>. Exp Ther Med 21: 146, 2021
APA
Li, Y., Shi, H., Chen, R., Zhou, S., Lei, S., & She, Y. (2021). Role of miRNAs and lncRNAs in dexamethasone‑induced myotube atrophy <em>in vitro</em>. Experimental and Therapeutic Medicine, 21, 146. https://doi.org/10.3892/etm.2020.9577
MLA
Li, Y., Shi, H., Chen, R., Zhou, S., Lei, S., She, Y."Role of miRNAs and lncRNAs in dexamethasone‑induced myotube atrophy <em>in vitro</em>". Experimental and Therapeutic Medicine 21.2 (2021): 146.
Chicago
Li, Y., Shi, H., Chen, R., Zhou, S., Lei, S., She, Y."Role of miRNAs and lncRNAs in dexamethasone‑induced myotube atrophy <em>in vitro</em>". Experimental and Therapeutic Medicine 21, no. 2 (2021): 146. https://doi.org/10.3892/etm.2020.9577