Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling

  • Authors:
    • Xiaoqing Xiao
    • Minghuan Huang
    • Chunyan Fan
    • Fuguo Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
  • Article Number: 157
    |
    Published online on: December 17, 2020
       https://doi.org/10.3892/etm.2020.9588
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Skin and in particular photoaging or premature aging, are caused by a variety of factors, including hormone imbalance and exposure to ultraviolet radiation. The aim of the present study was to explore the roles of dual oxidase 2 (DUOX2) and related NF‑κB signals in skin photoaging. Cell models of photoaging were constructed by irradiating human skin fibroblast lines (HSF2) with ultraviolet B (UVB) of different doses (0, 15, 30 and 60 mj/cm2). The cell counting kit‑8 (CCK8) was used to determine cell proliferation. Flow cytometry was used to determine the production of reactive oxygen species (ROS). A biochemical method was to determine the content of hydrogen peroxide, and the quantitative PCR (qPCR) was used to determine the expression of matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9), Col‑Ⅰ and α‑SMA in the cells. Enzyme‑linked immunosorbent assay (ELISA) was used to determine the expression of tumor necrosis factor‑α (TNF‑α) and interleukin‑6 (IL‑6). Western blot analysis was performed to determine the expression of DUOX2, p65 and p‑p65.The results showed that,UVB irradiation dose‑ and time‑dependently inhibited the proliferation of HSF2 cells. Cellular inflammatory response, ROS production and hydrogen peroxide increase was promoted. Col‑Ⅰ and α‑SMA were downregulated, MMP2 and MMP9 were upregulated, and the phosphorylation of NF‑κB p65 was promoted. The above indicators were all reversed by interference with DUOX2. Overexpression of DUOX2 has an effect that is similar to UVB irradiation, but the effects can be significantly weakened by NF‑κB inhibitor, NAC. Upregulation of DUOX2 expression plays a crucial role in UVB‑induced aging of HSF2 cells. The specific mechanism is related to the promotion of ROS production and cellular inflammatory response and activation of NF‑κB signals.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Krutmann J, Bouloc A, Sore G, Bernard BA and Passeron T: The skin aging exposome. J Dermatol Sci. 85:152–161. 2017.PubMed/NCBI View Article : Google Scholar

2 

Huang CY, Lin YT, Kuo HC, Chiou WF and Lee MH: Compounds isolated from Eriobotrya deflexa leaves protect against ultraviolet radiation B-induced photoaging in human fibroblasts. J Photochem Photobiol. 175:244–253. 2017.PubMed/NCBI View Article : Google Scholar

3 

Nichols JA and Katiyar SK: Skin photoprotection by natural polyphenols: Anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res. 302:71–83. 2010.PubMed/NCBI View Article : Google Scholar

4 

Goldman MP, Weiss RA and Weiss MA: Intense pulsed light as a nonablative approach to photoaging. Dermatol Surg. 31:1179–1187. 2005.PubMed/NCBI View Article : Google Scholar

5 

Mora Huertas AC, Schmelzer CE, Hoehenwarter W, Heyroth F and Heinz A: Molecular-level insights into aging processes of skin elastin. Biochimie. 128-129:163–173. 2016.PubMed/NCBI View Article : Google Scholar

6 

Lei X, Liu B, Han W, Ming M and He YY: UVB-Induced p21 degradation promotes apoptosis of human keratinocytes. Photochem Photobiol Sci. 9:1640–1648. 2010.PubMed/NCBI View Article : Google Scholar

7 

Duncan FJ, Martin JR, Wulff BC, Stoner GD, Tober KL, Oberyszyn TM, Kusewitt DF and Van Buskirk AM: Topical treatment with black raspberry extract reduces cutaneous UVB-induced carcinogenesis and inflammation. Cancer Prev Res (Phila). 2:665–672. 2009.PubMed/NCBI View Article : Google Scholar

8 

Sharma SD, Meeran SM and Katiyar SK: Dietary grape seed proanthocyanidins inhibit UVB-induced oxidative stress and activation of mitogen-activated protein kinases and nuclear factor-kappaB signaling in in vivo SKH-1 hairless mice. Mol Cancer Ther. 6:995–1005. 2007.PubMed/NCBI View Article : Google Scholar

9 

Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S and Voorhees JJ: Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature. 379:335–339. 1996.PubMed/NCBI View Article : Google Scholar

10 

Poon F, Kang S and Chien AL: Mechanisms and treatments of photoaging. Photodermatol Photoimmunol Photomed. 31:65–74. 2015.PubMed/NCBI View Article : Google Scholar

11 

Yokoyama T, Komori A, Nakamura M, Takii Y, Kamihira T, Shimoda S, Mori T, Fujiwara S, Koyabu M, Taniguchi K, et al: Human intrahepatic biliary epithelial cells function in innate immunity by producing IL-6 and IL-8 via the TLR4-NF-kappaB and -MAPK signaling pathways. Liver Int. 26:467–476. 2006.PubMed/NCBI View Article : Google Scholar

12 

Orlando A, Cazzaniga E, Tringali M, Gullo F, Becchetti A, Minniti S, Taraballi F, Tasciotti E and Re F: Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner. Int J Nanomedicine. 12:3547–3559. 2017.PubMed/NCBI View Article : Google Scholar

13 

Matsumura Y and Ananthaswamy HN: Toxic effects of ultraviolet radiation on the skin. Toxicol Appl Pharmacol. 195:298–308. 2004.PubMed/NCBI View Article : Google Scholar

14 

Bedard K and Krause KH: The NOX family of ROS-generating NADPH oxidases: Physiology and pathophysiology. Physiol Rev. 87:245–313. 2007.PubMed/NCBI View Article : Google Scholar

15 

Song H, Zhao H, Yang L, Li L, Zhang T, Pan J, Meng Y, Shen W and Yuan Y: Achyranthes bidentata polypeptides promotes migration of Schwann cells via NOX4/DUOX2-dependent ROS production in rats. Neurosci Lett. 696:99–107. 2019.PubMed/NCBI View Article : Google Scholar

16 

Luo J, Tang M, Huang J, He BC, Gao JL, Chen L, Zuo GW, Zhang W, Luo Q, Shi Q, et al: TGFbeta/BMP type I receptors ALK1 and ALK2 are essential for BMP9-induced osteogenic signaling in mesenchymal stem cells. J Biol Chem. 285:29588–29598. 2010.PubMed/NCBI View Article : Google Scholar

17 

Caldefie-Chézet F, Walrand S, Moinard C, Tridon A, Chassagne J and Vasson MP: Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction. Clin Chim Acta. 319:9–17. 2002.PubMed/NCBI View Article : Google Scholar

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

19 

Ko EY, Cho SH, Kwon SH, Eom CY, Jeong MS, Lee W, Kim SY, Heo SJ, Ahn G, Lee KP, et al: The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos. Fish Shellfish Immunol. 68:525–529. 2017.PubMed/NCBI View Article : Google Scholar

20 

Hsieh HY, Lee WC, Senadi GC, Hu WP, Liang JJ, Tsai TR, Chou YW, Kuo KK, Chen CY and Wang JJ: Discovery, synthetic methodology, and biological evaluation for antiphotoaging activity of bicyclic[1,2,3]triazoles: In vitro and in vivo studies. J Med Chem. 56:5422–5435. 2013.PubMed/NCBI View Article : Google Scholar

21 

Wlaschek M, Tantcheva-Poór I, Naderi L, Ma W, Schneider LA, Razi-Wolf Z, Schüller J and Scharffetter-Kochanek K: Solar UV irradiation and dermal photoaging. J Photochem Photobiol B. 63:41–51. 2001.PubMed/NCBI View Article : Google Scholar

22 

Zhuang Y and Lyga J: Inflammaging in skin and other tissues-the roles of complement system and macrophage. Inflamm Allergy Drug Targets. 13:153–161. 2014.PubMed/NCBI View Article : Google Scholar

23 

Fulop T, Larbi A, Dupuis G, Le Page A, Frost EH, Cohen AA, Witkowski JM and Franceschi C: Immunosenescence and inflamm-aging as two sides of the same coin: Friends or foes? Front Immunol. 8(1960)2017.PubMed/NCBI View Article : Google Scholar

24 

PLOS ONE Editors. Retraction: Anti-wrinkle effect of magnesium lithospermate B from salvia miltiorrhiza BUNGE: Inhibition of MMPs via NF-κB signaling. PLoS One. 14(e0216473)2019.PubMed/NCBI View Article : Google Scholar

25 

Staniforth V, Huang WC, Aravindaram K and Yang NS: Ferulic acid, a phenolic phytochemical, inhibits UVB-induced matrix metalloproteinases in mouse skin via posttranslational mechanisms. J Nutr Biochem. 23:443–451. 2012.PubMed/NCBI View Article : Google Scholar

26 

Buvelot H, Jaquet V and Krause KH: Mammalian NADPH oxidases. Methods Mol Biol. 1982:17–36. 2019.PubMed/NCBI View Article : Google Scholar

27 

Kang KA, Ryu YS, Piao MJ, Shilnikova K, Kang HK, Yi JM, Boulanger M, Paolillo R, Bossis G, Yoon SY, et al: DUOX2-mediated production of reactive oxygen species induces epithelial mesenchymal transition in 5-fluorouracil resistant human colon cancer cells. Redox Biol. 17:224–235. 2018.PubMed/NCBI View Article : Google Scholar

28 

Wu Y, Konaté MM, Lu J and Makhlouf H: IL-4 and IL-17A cooperatively promote hydrogen peroxide production, oxidative DNA damage, and upregulation of dual oxidase 2 in human colon and pancreatic cancer cells. J Immunol. 203:2532–2544. 2019.PubMed/NCBI View Article : Google Scholar

29 

Ewald CY: Redox signaling of NADPH oxidases regulates oxidative stress responses, immunity and aging. Antioxidants (Basel). 7(130)2018.PubMed/NCBI View Article : Google Scholar

30 

Little AC, Sulovari A, Danyal K, Heppner DE, Seward DJ and van der Vliet A: Paradoxical roles of dual oxidases in cancer biology. Free Radic Biol Med. 110:117–132. 2017.PubMed/NCBI View Article : Google Scholar

31 

Harper RW, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H and Wu R: Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium. FEBS Lett. 579:4911–4917. 2005.PubMed/NCBI View Article : Google Scholar

32 

Moldogazieva NT, Mokhosoev IM, Feldman NB and Lutsenko SV: ROS and RNS signalling: Adaptive redox switches through oxidative/nitrosative protein modifications. Free Radic Res. 52:507–543. 2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xiao X, Huang M, Fan C and Zuo F: DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling. Exp Ther Med 21: 157, 2021.
APA
Xiao, X., Huang, M., Fan, C., & Zuo, F. (2021). DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling. Experimental and Therapeutic Medicine, 21, 157. https://doi.org/10.3892/etm.2020.9588
MLA
Xiao, X., Huang, M., Fan, C., Zuo, F."DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling". Experimental and Therapeutic Medicine 21.2 (2021): 157.
Chicago
Xiao, X., Huang, M., Fan, C., Zuo, F."DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling". Experimental and Therapeutic Medicine 21, no. 2 (2021): 157. https://doi.org/10.3892/etm.2020.9588
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao X, Huang M, Fan C and Zuo F: DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling. Exp Ther Med 21: 157, 2021.
APA
Xiao, X., Huang, M., Fan, C., & Zuo, F. (2021). DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling. Experimental and Therapeutic Medicine, 21, 157. https://doi.org/10.3892/etm.2020.9588
MLA
Xiao, X., Huang, M., Fan, C., Zuo, F."DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling". Experimental and Therapeutic Medicine 21.2 (2021): 157.
Chicago
Xiao, X., Huang, M., Fan, C., Zuo, F."DUOX2 participates in skin aging induced by UVB in HSF2 cells by activating NF‑κB signaling". Experimental and Therapeutic Medicine 21, no. 2 (2021): 157. https://doi.org/10.3892/etm.2020.9588
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team