Difference in serum miRNA expression between immunoglobulin‑sensitive and ‑insensitive incomplete Kawasaki disease patients

Wang,Yan; Li,Chunli; Niu,Ling; Fu,Mingyu; Tian,Jing; An,Xinjiang

doi:10.3892/etm.2020.9593

Difference in serum miRNA expression between immunoglobulin‑sensitive and ‑insensitive incomplete Kawasaki disease patients

Authors:
- Yan Wang
- Chunli Li
- Ling Niu
- Mingyu Fu
- Jing Tian
- Xinjiang An
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Affiliations: Department of Cardiovascular Medicine, Xuzhou Children's Hospital Affiliated to Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China
Published online on: December 18, 2020 https://doi.org/10.3892/etm.2020.9593
Article Number: 162
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the expression of microRNAs (miRNAs/miRs) and inflammatory factors in patients with immunoglobulin‑sensitive and IVIG‑insensitive incomplete Kawasaki disease (KD). One hundred and eighty‑five patients with incomplete KD were included as the study group (KD group), and 182 patients with respiratory infection as the control group. Neutrophil to lymphocyte ratio (NLR), C‑reactive protein (CRP) levels, alanine aminotransferase (ALT), aspartate aminotransferase (AST), white blood cell count (WBC), hemoglobin level (Hb), platelet count (PLT) and T cell subsets (CD3⁺, CD3⁺ CD4⁺) were compared. Patients in the KD group received aspirin (30 mg/kg orally daily) and gamma globulin (IVIG, 1 g/kg intravenously daily). According to the sensitivity to IVIG, patients were divided into IVIG‑sensitive group and IVIG‑insensitive KD group. The relative expression levels of miRNA‑21, miRNA‑145, miRNA‑155 and miRNA‑199b‑5p in the serum were detected by RT‑qPCR. Serum TNF‑α, IL‑6 and IL‑1β levels were assessed using ELISA. Before treatment, the neutrophil to lymphocyte ratio (NLR), levels C‑reactive protein, and leukocytes in the KD group were significantly higher compared with the control group (P<0.05). After medical intervention, the relative expression of miRNA‑21, miRNA‑145 and miRNA‑155 in the serum of patients in IVIG‑sensitive and IVIG‑insensitive KD groups were increased when compared with these levels in the control group (P<0.05). Meanwhile, the relative expression of miRNA‑199b‑5p was decreased (P<0.05). Compared with the IVIG‑sensitive KD group, the relative expression levels of miRNA‑145 and miRNA‑155 were increased in the serum of patients in the IVIG‑insensitive KD group (P<0.05). Compared with the control group, the levels of TNF‑α, IL‑6 and IL‑1β were increased in the serum of patients in the IVIG‑sensitive and IVIG‑insensitive KD groups (P<0.05). Compared with the IVIG‑sensitive KD group, the serum levels of TNF‑α and IL‑6 were increased in patients of the IVIG‑insensitive KD group (P<0.05). Except for NLR and CRP, there were differences in the expression of peripheral blood miRNA‑145, miRNA‑155 and serum TNF‑α and IL‑6 in patients with immunoglobulin‑sensitive and ‑insensitive incomplete KD.

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