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Article

Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells

  • Authors:
    • Sujin Lee
    • Dong-Sung Lee
    • Jun-Hyeog Jang
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, College of Medicine, Inha University, Incheon 22212, Republic of Korea, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea
  • Article Number: 166
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    Published online on: December 21, 2020
       https://doi.org/10.3892/etm.2020.9597
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Abstract

The extracellular matrix components laminin and elastin serve key roles in stem cell therapy. Elastin‑like polypeptides (ELPs), derived from a soluble form of elastin, affect the proliferation and differentiation of various types of cells. In the present study, a novel protein was designed containing globular domains 1‑3 of laminin α5 (Lα5LG1‑3) fused to ELPs (Lα5LG1‑3/ELP). Lα5LG1‑3/ELP was expressed in Escherichia coli and displayed a molecular size of ~70 kDa on 12% SDS‑polyacrylamide gels. The cellular activities, such as cellular adhesion (adhesion assay) and proliferation (MTT cytotoxicity assay), of human mesenchymal stem cells (hMSCs) treated with 1 µg/ml of Lα5LG1‑3/ELP were enhanced compared with those of untreated cells. Additionally, the number of undifferentiated hMSCs and their degree of stemness were assessed based on the gene expression levels of the stem cell markers cluster differentiation 90 (CD90), endoglin (CD105) and CD73. The expression levels of these markers were upregulated by 2.42‑, 2.29‑ and 1.92‑fold, respectively, in the hMSCs treated with Lα5LG1‑3/ELP compared with the levels in untreated controls. Thus, Lα5LG1‑3/ELP may be used to enhance the viability of hMSCs and preserve their undifferentiated state, whereby the clinical applications of hMSCs may be improved.
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Copy and paste a formatted citation
Spandidos Publications style
Lee S, Lee D and Jang J: Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Exp Ther Med 21: 166, 2021.
APA
Lee, S., Lee, D., & Jang, J. (2021). Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Experimental and Therapeutic Medicine, 21, 166. https://doi.org/10.3892/etm.2020.9597
MLA
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21.2 (2021): 166.
Chicago
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21, no. 2 (2021): 166. https://doi.org/10.3892/etm.2020.9597
Copy and paste a formatted citation
x
Spandidos Publications style
Lee S, Lee D and Jang J: Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Exp Ther Med 21: 166, 2021.
APA
Lee, S., Lee, D., & Jang, J. (2021). Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Experimental and Therapeutic Medicine, 21, 166. https://doi.org/10.3892/etm.2020.9597
MLA
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21.2 (2021): 166.
Chicago
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21, no. 2 (2021): 166. https://doi.org/10.3892/etm.2020.9597
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