Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells

  • Authors:
    • Sujin Lee
    • Dong-Sung Lee
    • Jun-Hyeog Jang
  • View Affiliations

  • Published online on: December 21, 2020     https://doi.org/10.3892/etm.2020.9597
  • Article Number: 166
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The extracellular matrix components laminin and elastin serve key roles in stem cell therapy. Elastin‑like polypeptides (ELPs), derived from a soluble form of elastin, affect the proliferation and differentiation of various types of cells. In the present study, a novel protein was designed containing globular domains 1‑3 of laminin α5 (Lα5LG1‑3) fused to ELPs (Lα5LG1‑3/ELP). Lα5LG1‑3/ELP was expressed in Escherichia coli and displayed a molecular size of ~70 kDa on 12% SDS‑polyacrylamide gels. The cellular activities, such as cellular adhesion (adhesion assay) and proliferation (MTT cytotoxicity assay), of human mesenchymal stem cells (hMSCs) treated with 1 µg/ml of Lα5LG1‑3/ELP were enhanced compared with those of untreated cells. Additionally, the number of undifferentiated hMSCs and their degree of stemness were assessed based on the gene expression levels of the stem cell markers cluster differentiation 90 (CD90), endoglin (CD105) and CD73. The expression levels of these markers were upregulated by 2.42‑, 2.29‑ and 1.92‑fold, respectively, in the hMSCs treated with Lα5LG1‑3/ELP compared with the levels in untreated controls. Thus, Lα5LG1‑3/ELP may be used to enhance the viability of hMSCs and preserve their undifferentiated state, whereby the clinical applications of hMSCs may be improved.
View Figures
View References

Related Articles

Journal Cover

February-2021
Volume 21 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee S, Lee D and Jang J: Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Exp Ther Med 21: 166, 2021
APA
Lee, S., Lee, D., & Jang, J. (2021). Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells. Experimental and Therapeutic Medicine, 21, 166. https://doi.org/10.3892/etm.2020.9597
MLA
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21.2 (2021): 166.
Chicago
Lee, S., Lee, D., Jang, J."Recombinant laminin α5 LG1-3 domains support the stemness of human mesenchymal stem cells". Experimental and Therapeutic Medicine 21, no. 2 (2021): 166. https://doi.org/10.3892/etm.2020.9597