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ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide

  • Authors:
    • Xueru Chen
    • Zijin Xiang
    • Dangchi Li
    • Xiuting Zhu
    • Xiangdong Peng
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Jiangxi University of Technology High School, Nanchang, Jiangxi 330029, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 175
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    Published online on: December 28, 2020
       https://doi.org/10.3892/etm.2020.9606
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Abstract

Molecular‑targeted therapy has had a significant impact on glioma. Notably, actin‑like 6A (ACTL6A) has been indicated to be essential for embryonic development and tumor progression. However, the role of ACTL6A in glioma remains unclear. The present study aimed to investigate the effects of ACTL6A on glioma cell migration and sensitivity to temozolomide (TMZ). The expression levels of ACTL6A were analyzed in patients with glioma, and survival curves were created using data from The Cancer Genome Atlas. U251 and T98G cells were transfected with short hairpin (sh)RNA for use in loss‑of‑function experiments to investigate the biological function and molecular mechanisms of ACTL6A. Furthermore, an MTT assay was used to assess the effect of ACTL6A on the sensitivity of glioma cells to TMZ. The results demonstrated that ACTL6A was expressed at higher levels in glioma tissues compared with normal brain tissues. Furthermore, high expression of ACTL6A was associated with a poor prognosis. The knockdown of ACTL6A significantly inhibited the migration phenotype in glioma cells and significantly decreased the levels of phosphorylated AKT in glioma cells. The AKT signaling activator SC79 partly attenuated the inhibitory effects of ACTL6A shRNA on glioma cell migration. Additionally, the knockdown of ACTL6A enhanced the sensitivity of glioma cells to TMZ. In conclusion, these results suggest that ACTL6A knockdown inhibited the migration of human glioma cells, at least in part through inactivation of the AKT signaling pathway, and increased the sensitivity of glioma cells to TMZ. Therefore, ACTL6A may be a potential therapeutic target for glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Xiang Z, Li D, Zhu X and Peng X: ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide. Exp Ther Med 21: 175, 2021.
APA
Chen, X., Xiang, Z., Li, D., Zhu, X., & Peng, X. (2021). ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide. Experimental and Therapeutic Medicine, 21, 175. https://doi.org/10.3892/etm.2020.9606
MLA
Chen, X., Xiang, Z., Li, D., Zhu, X., Peng, X."ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide". Experimental and Therapeutic Medicine 21.2 (2021): 175.
Chicago
Chen, X., Xiang, Z., Li, D., Zhu, X., Peng, X."ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide". Experimental and Therapeutic Medicine 21, no. 2 (2021): 175. https://doi.org/10.3892/etm.2020.9606
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Xiang Z, Li D, Zhu X and Peng X: ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide. Exp Ther Med 21: 175, 2021.
APA
Chen, X., Xiang, Z., Li, D., Zhu, X., & Peng, X. (2021). ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide. Experimental and Therapeutic Medicine, 21, 175. https://doi.org/10.3892/etm.2020.9606
MLA
Chen, X., Xiang, Z., Li, D., Zhu, X., Peng, X."ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide". Experimental and Therapeutic Medicine 21.2 (2021): 175.
Chicago
Chen, X., Xiang, Z., Li, D., Zhu, X., Peng, X."ACTL6A knockdown inhibits cell migration by suppressing the AKT signaling pathway and enhances the sensitivity of glioma cells to temozolomide". Experimental and Therapeutic Medicine 21, no. 2 (2021): 175. https://doi.org/10.3892/etm.2020.9606
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