Open Access

Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats

  • Authors:
    • Hongfang Li
    • Yunyan Tang
    • Weipeng Wei
    • Chengchen Yin
    • Fushan Tang
  • View Affiliations

  • Published online on: April 2, 2021     https://doi.org/10.3892/etm.2021.10020
  • Article Number: 588
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Xiaochaihu decoction is one of the most important traditional Chinese medicines that is widely used with other drugs in clinical practice, and may cause drug‑drug interactions. However, there is not sufficient experimental evidence for the effects of Xiaochaihu decoction on cytochrome P450s (CYPs). The aim of the present study was to investigate the effects of Xiaochaihu decoction on the mRNA and protein levels of hepatic CYPs. Eighty normal male Sprague‑Dawley (SD) rats were randomly divided into two groups based on body weight and duration of drug administration (3 and 6 days). Each group was further divided into subgroups: Control group (2 ml 5‰ CMC‑Na); hepatic enzyme inducer group (50 mg/kg/day rifampicin); and experimental groups (Xiaochaihu decoction: Low dose, 1.7 g/kg/day; medium dose, 3.4 g/kg/day; high dose, 6.8 g/kg/day). The effects of Xiaochaihu decoction on Cyp1a2, Cyp3a1, Cyp2d6, and Cyp1b1 mRNA and protein expression in rats were evaluated using reverse transcription quantitative reverse transcription polymerase chain reaction and western blot analysis. After 3 days, medium dose of Xiaochaihu decoction inhibited the mRNA and protein expression of Cyp1a2, Cyp3a1 and Cyp1b1. In addition, after 6 days, Xiaochaihu decoction induced Cyp3a1 mRNA expression at low and medium doses; Cyp2d6 mRNA expression at low and high doses; and Cyp2d6 protein expression at high doses. Nonetheless, the gene and protein expression of Cyp1b1 was not affected at any dose. The findings of the present study may provide insights into potential drug‑drug interactions associated with Xiaochaihu decoction.
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June-2021
Volume 21 Issue 6

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Copy and paste a formatted citation
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Spandidos Publications style
Li H, Tang Y, Wei W, Yin C and Tang F: Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats. Exp Ther Med 21: 588, 2021
APA
Li, H., Tang, Y., Wei, W., Yin, C., & Tang, F. (2021). Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats. Experimental and Therapeutic Medicine, 21, 588. https://doi.org/10.3892/etm.2021.10020
MLA
Li, H., Tang, Y., Wei, W., Yin, C., Tang, F."Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats". Experimental and Therapeutic Medicine 21.6 (2021): 588.
Chicago
Li, H., Tang, Y., Wei, W., Yin, C., Tang, F."Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats". Experimental and Therapeutic Medicine 21, no. 6 (2021): 588. https://doi.org/10.3892/etm.2021.10020