Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2021 Volume 21 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2021 Volume 21 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Role of Notch, IL‑1 and leptin expression in colorectal cancer

  • Authors:
    • Nilufer Erkasap
    • Rumeysa Ozyurt
    • Mete Ozkurt
    • Serdar Erkasap
    • Fatih Yasar
    • Enver Ihtiyar
    • Evrim Ciftci
    • Funda Canaz
    • Ertugrul Colak
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Odunpazari, Eskisehir 26040, Turkey, Department of General Surgery, Eskisehir Osmangazi University Medical Faculty, Odunpazari, Eskisehir 26040, Turkey, Department of Pathology, Eskisehir Osmangazi University Medical Faculty, Odunpazari, Eskisehir 26040, Turkey, Department of Biostatistics, Eskisehir Osmangazi University Medical Faculty, Odunpazari, Eskisehir 26040, Turkey
    Copyright: © Erkasap et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 600
    |
    Published online on: April 11, 2021
       https://doi.org/10.3892/etm.2021.10032
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

An increasing number of studies have shown that angiogenesis has an important role in the progression of cancer. The growth of a new network of blood vessels is crucial for tumor growth and metastasis, which is promoted by several proangiogenic factors. Leptin, an essential adipokine that is secreted from fat tissue, is one of these pro‑angiogenic factors. It has been shown that the inhibition of leptin‑induced angiogenesis resulted in decreased levels of vascular endothelial growth factor (VEGF)/VEGFR2, hypoxia inducible factor (HIF) 1α, NF‑κB, IL‑1 and Notch and reduced the tumor growth in breast cancer. Leptin induces angiogenesis in breast cancer either by upregulating VEGFR2 in endothelial cells or by increasing VEGF/VEGFR2 expression through the Notch, IL‑1 and leptin crosstalk outcome (NILCO) pathway. NILCO is a novel mechanism that interacts with proinflammatory and proangiogenic signals, which are critical for cell proliferation and angiogenesis in cancer. Several studies have shown that components of NILCO may affect human cancer incidence and progression. However, to the best of our knowledge, the interactions between Notch, IL‑1 and leptin in human colorectal cancer have not been yet studied at the molecular level. The aim of the present study was to investigate the expression levels of genes related to the NILCO pathway in human colorectal cancer specimens. The current results demonstrated that leptin, leptin receptor (ObR) b, Notch‑1, Notch‑4, IL‑1α, IL‑1β, IL‑1R, IL‑6, JAK‑2, STAT‑1, STAT‑3, VEGFA, VEGFR1, VEGFR2, TNF‑α and NF‑κB mRNA expression levels in the cancer tissue were increased compared with the normal tissue. No significant changes in the mRNA expression levels of Jagged‑1, HIF‑1α and TNF receptor 1 were observed. Western blotting revealed that the protein expression levels of IκB were increased in the cancer tissue compared with normal tissue, whereas HIF‑1α and phosphorylated STAT‑1 levels were decreased. IL‑6 and VEGFA plasma concentrations were statistically raised and the leptin plasma concentration was also raised, although significantly, patients with cancer compared with control individuals. Together, the present findings indicated that Notch, IL‑1 and leptin may serve a crucial role in the development of colorectal cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

van Kruijsdijk RC, van der Wall E and Visseren FL: Obesity and cancer: The role of dysfunctional adipose tissue. Cancer Epidemiol Biomarkers Prev. 18:2569–2578. 2009.PubMed/NCBI View Article : Google Scholar

2 

Zhou W, Guo S and Gonzalez-Perez RR: Leptin pro-angiogenic signature in breast cancer is linked to IL-1 signalling. Br J Cancer. 104:128–137. 2011.PubMed/NCBI View Article : Google Scholar

3 

Gonzalez-Perez RR, Lanier V and Newman G: Leptin's pro-angiogenic signature in breast cancer. Cancers (Basel). 5:1140–1162. 2013.PubMed/NCBI View Article : Google Scholar

4 

Guo S and Gonzalez-Perez RR: Notch, IL-1 and leptin crosstalk outcome (NILCO) is critical for leptin-induced proliferation, migration and VEGF/VEGFR-2 expression in breast cancer. PLoS One. 6(e21467)2011.PubMed/NCBI View Article : Google Scholar

5 

Wang B, Wood IS and Trayhurn P: Hypoxia induces leptin gene expression and secretion in human preadipocytes: Differential effects of hypoxia on adipokine expression by preadipocytes. J Endocrinol. 198:127–134. 2008.PubMed/NCBI View Article : Google Scholar

6 

Elaraj DM, Weinreich DM, Varghese S, Puhlmann M, Hewitt SM, Carroll NM, Feldman ED, Turner EM and Alexander HR: The role of interleukin 1 in growth and metastasis of human cancer xenografts. Clin Cancer Res. 12:1088–1096. 2006.PubMed/NCBI View Article : Google Scholar

7 

Lipsey CC, Harbuzariu A, Daley-Brown D and Gonzalez-Perez RR: Oncogenic role of leptin and Notch interleukin-1 leptin crosstalk outcome in cancer. World J Methodol. 6:43–55. 2016.PubMed/NCBI View Article : Google Scholar

8 

Daley-Brown D, Harbuzariu A, Kurian AA, Oprea-Ilies G and Gonzalez-Perez RR: Leptin-induced Notch and IL-1 signaling crosstalk in endometrial adenocarcinoma is associated with invasiveness and chemoresistance. World J Clin Oncol. 10:222–233. 2019.PubMed/NCBI View Article : Google Scholar

9 

Lee S, Trivedi U, Johnson C, Farquharson C and Bergkvist GT: Optimised isolation method for RNA extraction suitable for RNA sequencing from feline teeth collected in a clinical setting and at post mortem. Vet Res Commun. 43:17–27. 2019.PubMed/NCBI View Article : Google Scholar

10 

Rao X, Huang X, Zhou Z and Lin X: An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 3:71–85. 2013.PubMed/NCBI

11 

Cust AE, Stocks T, Lukanova A, Lundin E, Hallmans G, Kaaks R, Jonsson H and Stattin P: The influence of overweight and insulin resistance on breast cancer risk and tumour stage at diagnosis: A prospective study. Breast Cancer Res Treat. 113:567–576. 2009.PubMed/NCBI View Article : Google Scholar

12 

Wei EK, Giovannucci E, Fuchs CS, Willett WC and Mantzoros CS: Low plasma adiponectin levels and risk of colorectal cancer in men: A prospective study. J Natl Cancer Inst. 97:1688–1694. 2005.PubMed/NCBI View Article : Google Scholar

13 

Erkasap N, Ozkurt M, Erkasap S, Yasar F, Uzuner K, Ihtiyar E, Uslu S, Kara M and Bolluk O: Leptin receptor (Ob-R) mRNA expression and serum leptin concentration in patients with colorectal and metastatic colorectal cancer. Braz J Med Biol Res. 46:306–310. 2013.PubMed/NCBI View Article : Google Scholar

14 

Endo H, Hosono K, Uchiyama T, Sakai E, Sugiyama M, Takahashi H, Nakajima N, Wada K, Takeda K, Nakagama H and Nakajima A: Leptin acts as a growth factor for colorectal tumours at stages subsequent to tumour initiation in murine colon carcinogenesis. Gut. 60:1363–1371. 2011.PubMed/NCBI View Article : Google Scholar

15 

Wegler C, Ölander M, Wiśniewski JR, Lundquist P, Zettl K, Åsberg A, Hjelmesæth J, Andersson TB and Artursson P: Global variability analysis of mRNA and protein concentrations across and within human tissues. NAR Genom Bioinform. 2(lqz010)2019.PubMed/NCBI View Article : Google Scholar

16 

Rene Gonzalez R, Watters A, Xu Y, Singh UP, Mann DR, Rueda BR and Penichet ML: Leptin-signaling inhibition results in efficient anti-tumor activity in estrogen receptor positive or negative breast cancer. Breast Cancer Res. 11(R36)2009.PubMed/NCBI View Article : Google Scholar

17 

Noah TK and Shroyer NF: Notch in the intestine: Regulation of homeostasis and pathogenesis. Annu Rev Physiol. 75:263–288. 2013.PubMed/NCBI View Article : Google Scholar

18 

Artavanis-Tsakonas S, Rand MD and Lake RJ: Notch signaling: Cell fate control and signal integration in development. Science. 284:770–776. 1999.PubMed/NCBI View Article : Google Scholar

19 

Baron M: An overview of the Notch signalling pathway. Semin Cell Dev Biol. 14:113–119. 2003.PubMed/NCBI View Article : Google Scholar

20 

Guilmeau S, Flandez M, Mariadason JM and Augenlicht LH: Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: Implications for targeting Notch signaling. Oncogene. 29:992–1002. 2010.PubMed/NCBI View Article : Google Scholar

21 

Shen Y, Wang Q, Zhao Q and Zhou J: Leptin promotes the immune escape of lung cancer by inducing proinflammatory cytokines and resistance to apoptosis. Mol Med Rep. 2:295–299. 2009.PubMed/NCBI View Article : Google Scholar

22 

De Simone V, Pallone F, Monteleone G and Stolfi C: Role of T H 17 cytokines in the control of colorectal cancer. Oncoimmunology. 2(e26617)2013.PubMed/NCBI View Article : Google Scholar

23 

Mueller L, von Seggern L, Schumacher J, Goumas F, Wilms C, Braun F and Broering DC: TNF-alpha similarly induces IL-6 and MCP-1 in fibroblasts from colorectal liver metastases and normal liver fibroblasts. Biochem Biophys Res Commun. 397:586–5891. 2010.PubMed/NCBI View Article : Google Scholar

24 

Popivanova BK, Kitamura K, Wu Y, Kondo T, Kagaya T, Kaneko S, Oshima M, Fujii C and Mukaida N: Blocking TNF-alpha in mice reduces colorectal carcinogenesis associated with chronic colitis. J Clin Invest. 118:560–570. 2008.PubMed/NCBI View Article : Google Scholar

25 

Pereira SG and Oakley F: Nuclear factor-kappaB1: Regulation and function. Int J Biochem Cell Biol. 40:1425–1430. 2008.PubMed/NCBI View Article : Google Scholar

26 

Hayden MS and Ghosh S: Signaling to NF-kappaB. Genes Dev. 18:2195–2224. 2004.PubMed/NCBI View Article : Google Scholar

27 

Puvvada SD, Funkhouser WK, Greene K, Deal A, Chu H, Baldwin AS, Tepper JE and O'Neil BH: NF-kB and Bcl-3 activation are prognostic in metastatic colorectal cancer. Oncology. 78:181–188. 2010.PubMed/NCBI View Article : Google Scholar

28 

Kwon HC, Kim SH, Oh SY, Lee S, Kwon KA, Lee JH, Choi HJ, Park KJ, Lee HS, Roh MS and Kim HJ: Clinicopathological significance of nuclear factor-kappa B, HIF-1 alpha, and vascular endothelial growth factor expression in stage III colorectal cancer. Cancer Sci. 101:1557–1561. 2010.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Erkasap N, Ozyurt R, Ozkurt M, Erkasap S, Yasar F, Ihtiyar E, Ciftci E, Canaz F and Colak E: Role of Notch, IL‑1 and leptin expression in colorectal cancer. Exp Ther Med 21: 600, 2021.
APA
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E. ... Colak, E. (2021). Role of Notch, IL‑1 and leptin expression in colorectal cancer. Experimental and Therapeutic Medicine, 21, 600. https://doi.org/10.3892/etm.2021.10032
MLA
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E., Ciftci, E., Canaz, F., Colak, E."Role of Notch, IL‑1 and leptin expression in colorectal cancer". Experimental and Therapeutic Medicine 21.6 (2021): 600.
Chicago
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E., Ciftci, E., Canaz, F., Colak, E."Role of Notch, IL‑1 and leptin expression in colorectal cancer". Experimental and Therapeutic Medicine 21, no. 6 (2021): 600. https://doi.org/10.3892/etm.2021.10032
Copy and paste a formatted citation
x
Spandidos Publications style
Erkasap N, Ozyurt R, Ozkurt M, Erkasap S, Yasar F, Ihtiyar E, Ciftci E, Canaz F and Colak E: Role of Notch, IL‑1 and leptin expression in colorectal cancer. Exp Ther Med 21: 600, 2021.
APA
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E. ... Colak, E. (2021). Role of Notch, IL‑1 and leptin expression in colorectal cancer. Experimental and Therapeutic Medicine, 21, 600. https://doi.org/10.3892/etm.2021.10032
MLA
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E., Ciftci, E., Canaz, F., Colak, E."Role of Notch, IL‑1 and leptin expression in colorectal cancer". Experimental and Therapeutic Medicine 21.6 (2021): 600.
Chicago
Erkasap, N., Ozyurt, R., Ozkurt, M., Erkasap, S., Yasar, F., Ihtiyar, E., Ciftci, E., Canaz, F., Colak, E."Role of Notch, IL‑1 and leptin expression in colorectal cancer". Experimental and Therapeutic Medicine 21, no. 6 (2021): 600. https://doi.org/10.3892/etm.2021.10032
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team