Open Access

Transforming growth factor‑β1‑induced podocyte injury is associated with increased microRNA‑155 expression, enhanced inflammatory responses and MAPK pathway activation

  • Authors:
    • Xintong Zheng
    • Qiuhong Zhong
    • Xu Lin
    • Xianjun Gu
    • Xiaoyan Ling
    • Zhao Liang
    • Qing Qin
    • Xiuri Du
  • View Affiliations

  • Published online on: April 14, 2021     https://doi.org/10.3892/etm.2021.10052
  • Article Number: 620
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA‑155 (miR‑155) is associated with various diseases. However, the potential role of miR‑155 in early glomerular disease (EGD) remains elusive. In the present study, the clinical significance of urinary miR‑155 expression was explored in patients with EGD using receiver operating characteristic curve analysis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with transforming growth factor‑β1 (TGF‑β1) at different concentrations and durations. The gene expression levels of mRNAs and miR‑155 were detected using reverse transcription‑quantitative PCR. Synaptopodin, CD2‑associated protein (CD2AP), p38, and extracellular signal‑regulated kinase (Erk) 1/2 expressions were detected using western blotting. Cell supernatants were collected for assaying tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 concentrations using enzyme‑linked immunosorbent assay. The Pearson correlation analysis was used to analyze the correlation between miR‑155 levels and TNF‑α or IL‑6. It was found that miR‑155 levels in urine have high sensitivity and specificity in the diagnosis of EGD. Time‑ and dose‑dependent TGF‑β1 treatments downregulated synaptopodin and CD2AP expression levels, and activated the p38 and Erk 1/2 pathway. However, these effects were attenuated by p38 and Erk 1/2 phosphorylation inhibitors. Additionally, TNF‑α and IL‑6 secretions were elevated, and their concentrations were positively correlated with the expression of miR‑155 during podocyte injury. Thus, the present study indicated that miR‑155 is a potential biomarker for the diagnosis of EGD, and its expression is associated with the release of pro‑inflammatory cytokines and activation of mitogen‑activated protein kinase (MAPK) pathway in TGF‑β1‑induced podocyte injury. The present study suggests that the TGF‑β1/miR‑155/MAPK axis is a novel target in the mechanism of EGD.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zheng X, Zhong Q, Lin X, Gu X, Ling X, Liang Z, Qin Q and Du X: Transforming growth factor‑β1‑induced podocyte injury is associated with increased microRNA‑155 expression, enhanced inflammatory responses and MAPK pathway activation. Exp Ther Med 21: 620, 2021
APA
Zheng, X., Zhong, Q., Lin, X., Gu, X., Ling, X., Liang, Z. ... Du, X. (2021). Transforming growth factor‑β1‑induced podocyte injury is associated with increased microRNA‑155 expression, enhanced inflammatory responses and MAPK pathway activation. Experimental and Therapeutic Medicine, 21, 620. https://doi.org/10.3892/etm.2021.10052
MLA
Zheng, X., Zhong, Q., Lin, X., Gu, X., Ling, X., Liang, Z., Qin, Q., Du, X."Transforming growth factor‑β1‑induced podocyte injury is associated with increased microRNA‑155 expression, enhanced inflammatory responses and MAPK pathway activation". Experimental and Therapeutic Medicine 21.6 (2021): 620.
Chicago
Zheng, X., Zhong, Q., Lin, X., Gu, X., Ling, X., Liang, Z., Qin, Q., Du, X."Transforming growth factor‑β1‑induced podocyte injury is associated with increased microRNA‑155 expression, enhanced inflammatory responses and MAPK pathway activation". Experimental and Therapeutic Medicine 21, no. 6 (2021): 620. https://doi.org/10.3892/etm.2021.10052