miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4
- Fuyong Wu
- Aiping Ji
- Zhenkun Zhang
- Jinfang Li
- Penglong Li
Affiliations: Third Department of Oncology, People's Hospital of Shouguang, Shouguang, Shandong 262700, P.R. China, Second Department of Oncology, People's Hospital of Shouguang, Shouguang, Shandong 262700, P.R. China, Department of Oncology, Yantai Laiyang Central Hospital, Laiyang, Shandong 265200, P.R. China
- Published online on: April 14, 2021 https://doi.org/10.3892/etm.2021.10054
Copyright: © Wu
et al. This is an open access article distributed under the
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Aberrant expression of microRNAs (miRNAs/miRs) plays a key role in the development of non‑small cell lung cancer (NSCLC). In the present study, lower miRNA (miR)‑491‑5p levels and a higher forkhead box P4 (FOXP4) mRNA level were observed in NSCLC tissues and cell lines, compared to adjacent tissues and the normal human lung epithelial cell line BEAS‑2B, respectively. A549 cell proliferation and migration were inhibited upon transfection of miR‑491‑5p mimics compared to miR‑negative control (NC) mimics. In addition, compared to miR‑NC mimics, overexpression of miR‑491‑5p decreased FOXP4 expression, while downregulation of miR‑491‑5p increased FOXP4 expression in A549 cells. The dual luciferase assay confirmed that the 3'untranslated region of FOXP4 was a target for miR‑491‑5p in A549 cells. Moreover, compared with the control short hairpin (sh)RNA, there was lower expression levels of TGF‑β and its downstream targets (MMP‑2 and MMP‑9) in the FOXP4 shRNA group. Similarly, compared to miR‑NC mimics, overexpression of miR‑491‑5p decreased MMP‑2 and MMP‑9 expression levels. In FOXP4‑knockdown A549 cells, overexpression of miR‑491‑5p showed little effect on cell proliferation/migration. In A549 cells, overexpression of FOXP4 partially reversed the miR‑491‑5p mimics‑induced inhibition on the cell proliferation and migration. These results may provide new insights into the role of miR‑491‑5p in NSCLC.