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miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4

  • Authors:
    • Fuyong Wu
    • Aiping Ji
    • Zhenkun Zhang
    • Jinfang Li
    • Penglong Li
  • View Affiliations / Copyright

    Affiliations: Third Department of Oncology, People's Hospital of Shouguang, Shouguang, Shandong 262700, P.R. China, Second Department of Oncology, People's Hospital of Shouguang, Shouguang, Shandong 262700, P.R. China, Department of Oncology, Yantai Laiyang Central Hospital, Laiyang, Shandong 265200, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 622
    |
    Published online on: April 14, 2021
       https://doi.org/10.3892/etm.2021.10054
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Abstract

Aberrant expression of microRNAs (miRNAs/miRs) plays a key role in the development of non‑small cell lung cancer (NSCLC). In the present study, lower miRNA (miR)‑491‑5p levels and a higher forkhead box P4 (FOXP4) mRNA level were observed in NSCLC tissues and cell lines, compared to adjacent tissues and the normal human lung epithelial cell line BEAS‑2B, respectively. A549 cell proliferation and migration were inhibited upon transfection of miR‑491‑5p mimics compared to miR‑negative control (NC) mimics. In addition, compared to miR‑NC mimics, overexpression of miR‑491‑5p decreased FOXP4 expression, while downregulation of miR‑491‑5p increased FOXP4 expression in A549 cells. The dual luciferase assay confirmed that the 3'untranslated region of FOXP4 was a target for miR‑491‑5p in A549 cells. Moreover, compared with the control short hairpin (sh)RNA, there was lower expression levels of TGF‑β and its downstream targets (MMP‑2 and MMP‑9) in the FOXP4 shRNA group. Similarly, compared to miR‑NC mimics, overexpression of miR‑491‑5p decreased MMP‑2 and MMP‑9 expression levels. In FOXP4‑knockdown A549 cells, overexpression of miR‑491‑5p showed little effect on cell proliferation/migration. In A549 cells, overexpression of FOXP4 partially reversed the miR‑491‑5p mimics‑induced inhibition on the cell proliferation and migration. These results may provide new insights into the role of miR‑491‑5p in NSCLC.
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1 

Torre LA, Sauer AM, Chen MS Jr, Kagawa-Singer M, Jemal A and Siegel RL: Cancer statistics for Asian Americans, Native Hawaiians, and Pacific Islanders, 2016: Converging incidence in males and females. CA Cancer J Clin. 66:182–202. 2016.PubMed/NCBI View Article : Google Scholar

2 

Chen Z, Fillmore CM, Hammerman PS, Kim CF and Wong KK: Non-small-cell lung cancers: A heterogeneous set of diseases. Nat Rev Cancer. 14:535–546. 2014.PubMed/NCBI View Article : Google Scholar

3 

Wood SL, Pernemalm M, Crosbie PA and Whetton AD: The role of the tumor-microenvironment in lung cancer-metastasis and its relationship to potential therapeutic targets. Cancer Treat Rev. 40:558–566. 2014.PubMed/NCBI View Article : Google Scholar

4 

Laskin JJ and Sandler AB: State of the art in therapy for non-small cell lung cancer. Cancer Invest. 23:427–442. 2005.PubMed/NCBI View Article : Google Scholar

5 

Lagos-Quintana M, Rauhut R, Lendeckel W and Tuschl T: Identification of novel genes coding for small expressed RNAs. Science. 294:853–858. 2001.PubMed/NCBI View Article : Google Scholar

6 

Paliouras AR, Monteverde T and Garofalo M: Oncogene-induced regulation of microRNA expression: Implications for cancer initiation, progression and therapy. Cancer Lett. 421:152–160. 2018.PubMed/NCBI View Article : Google Scholar

7 

Ye MF, Zhang JG, Guo TX and Pan XJ: MiR-504 inhibits cell proliferation and invasion by targeting LOXL2 in non small cell lung cancer. Biomed Pharmacother. 97:1289–1295. 2018.PubMed/NCBI View Article : Google Scholar

8 

Hao J, Zhao S, Zhang Y, Zhao Z, Ye R, Wen J and Li J: Emerging role of microRNAs in cancer and cancer stem cells. J Cell Biochem. 115:605–610. 2014.PubMed/NCBI View Article : Google Scholar

9 

Zhou Q, Huang SX, Zhang F, Li SJ, Liu C, Xi YY, Wang L, Wang X, He QQ, Sun CC and Li DJ: MicroRNAs: A novel potential biomarker for diagnosis and therapy in patients with non-small cell lung cancer. Cell Prolif. 50(e12394)2017.PubMed/NCBI View Article : Google Scholar

10 

Xiong K, Shao LH, Zhang HQ, Jin L, Wei W, Dong Z, Zhu YQ, Wu N, Jin SZ and Xue LX: MicroRNA-9 functions as a tumor suppressor and enhances radio-sensitivity in radio-resistant A549 cells by targeting neuropilin 1. Oncol Lett. 15:2863–2870. 2018.PubMed/NCBI View Article : Google Scholar

11 

Zhang Y, Wang Y and Wang J: MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH. Exp Ther Med. 15:2203–2211. 2018.PubMed/NCBI View Article : Google Scholar

12 

Kulda V, Svaton M, Mukensnabl P, Hrda K, Dvorak P, Houdek Z, Houfkova K, Vrzakova R, Babuska V, Pesek M and Pesta M: Predictive relevance of miR-34a, miR-224 and miR-342 in patients with advanced squamous cell carcinoma of the lung undergoing palliative chemotherapy. Oncol Lett. 15:592–599. 2018.PubMed/NCBI View Article : Google Scholar

13 

Zhang J, Wang T, Zhang Y, Wang H, Wu Y, Liu K and Pei C: Upregulation of serum miR-494 predicts poor prognosis in non-small cell lung cancer patients. Cancer Biomark. 21:763–768. 2018.PubMed/NCBI View Article : Google Scholar

14 

Cao Q, Mao ZD, Shi YJ, Chen Y, Sun Y, Zhang Q, Song L and Peng LP: MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway. Oncotarget. 7:77468–77481. 2016.PubMed/NCBI View Article : Google Scholar

15 

Luo J, Li H and Zhang C: MicroRNA-7 inhibits the malignant phenotypes of non-small cell lung cancer in vitro by targeting Pax6. Mol Med Rep. 12:5443–5448. 2015.PubMed/NCBI View Article : Google Scholar

16 

Mou K, Gu W, Gu C, Zhang J, Qwang W, Ren G and Tian J: Relationship between miR-7 expression and treatment outcomes with gefitinib in non-small cell lung cancer. Oncol Lett. 12:4613–4617. 2016.PubMed/NCBI View Article : Google Scholar

17 

Teufel A, Wong EA, Mukhopadhyay M, Malik N and Westphal H: FoxP4, a novel forkhead transcription factor. Biochim Biophys Acta. 1627:147–152. 2003.PubMed/NCBI View Article : Google Scholar

18 

Tian W, Liu J, Pei B, Wang X, Guo Y and Yuan L: Identification of miRNAs and differentially expressed genes in early phase non-small cell lung cancer. Oncol Rep. 35:2171–2176. 2016.PubMed/NCBI View Article : Google Scholar

19 

Yin Z, Ding H, He E, Chen J and Li M: Up-regulation of microRNA-491-5p suppresses cell proliferation and promotes apoptosis by targeting FOXP4 in human osteosarcoma. Cell Prolif. 50(e12308)2017.PubMed/NCBI View Article : Google Scholar

20 

Wang G and Sun Y, He Y, Ji C, Hu B and Sun Y: MicroRNA-338-3p inhibits cell proliferation in hepatocellular carcinoma by target forkhead box P4 (FOXP4). Int J Clin Exp Pathol. 8:337–344. 2015.PubMed/NCBI

21 

Yang T, Li H, Thakur A, Chen T, Xue J, Li D and Chen M: FOXP4 modulates tumor growth and independently associates with miR-138 in non-small cell lung cancer cells. Tumour Biol. 36:8185–8191. 2015.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Sin C, Li H and Crawford DA: Transcriptional regulation by FOXP1, FOXP2, and FOXP4 dimerization. J Mol Neurosci. 55:437–448. 2015.PubMed/NCBI View Article : Google Scholar

24 

Song XL, Tang Y, Lei XH, Zhao SC and Wu ZQ: miR-618 inhibits prostate cancer migration and invasion by targeting FOXP2. J Cancer. 8:2501–2510. 2017.PubMed/NCBI View Article : Google Scholar

25 

Da C, Liu Y, Zhan Y, Liu K and Wang R: Nobiletin inhibits epithelial-mesenchymal transition of human non-small cell lung cancer cells by antagonizing the TGF-β1/Smad3 signaling pathway. Oncol Rep. 35:2767–2774. 2016.PubMed/NCBI View Article : Google Scholar

26 

Hui Z, Yiling C, Wenting Y, XuQun H, ChuanYi Z and Hui L: miR-491-5p functions as a tumor suppressor by targeting JMJD2B in ERα-positive breast cancer. FEBS Lett. 589:812–821. 2015.PubMed/NCBI View Article : Google Scholar

27 

Xu Y, Hou R, Lu Q, Zhang Y, Chen L, Zheng Y and Hu B: MiR-491-5p negatively regulates cell proliferation and motility by targeting PDGFRA in prostate cancer. Am J Cancer Res. 7:2545–2553. 2017.PubMed/NCBI

28 

Sun R, Liu Z, Tong D, Yang Y, Guo B, Wang X, Zhao L and Huang C: miR-491-5p, mediated by Foxi1, functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer. Cell Death Dis. 8(e2714)2017.PubMed/NCBI View Article : Google Scholar

29 

Gong F, Ren P, Zhang Y, Jiang J and Zhang H: MicroRNAs-491-5p suppresses cell proliferation and invasion by inhibiting IGF2BP1 in non-small cell lung cancer. Am J Transl Res. 8:485–495. 2016.PubMed/NCBI

30 

Sun D, Han S, Liu C, Zhou R, Sun W, Zhang Z and Qu J: Microrna-199a-5p functions as a tumor suppressor via suppressing connective tissue growth factor (CTGF) in follicular thyroid carcinoma. Med Sci Monit. 22:1210–1217. 2016.PubMed/NCBI View Article : Google Scholar

31 

Zhang Q, Li Q, Xu T, Jiang H and Xu LG: miR-491-5p suppresses cell growth and invasion by targeting Notch3 in nasopharyngeal carcinoma. Oncol Rep. 35:3541–3547. 2016.PubMed/NCBI View Article : Google Scholar

32 

Pirooz HJ, Jafari N, Rastegari M, Fathi-Roudsari M, Tasharrofi N, Shokri G, Tamadon M, Sazegar H and Kouhkan F: Functional SNP in microRNA-491-5p binding site of MMP9 3'-UTR affects cancer susceptibility. J Cell Biochem. 119:5126–5134. 2018.PubMed/NCBI View Article : Google Scholar

33 

Stetler-Stevenson WG: The role of matrix metalloproteinases in tumor invasion, metastasis, and angiogenesis. Surg Oncol Clin N Am. 10:383–392. 2001.PubMed/NCBI

34 

Kim S, Lee J, You D, Jeong Y, Jeon M, Yu J, Kim SW, Nam SJ and Lee JE: Berberine suppresses cell motility through downregulation of TGF-β1 in triple negative breast cancer cells. Cell Physiol Biochem. 45:795–807. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wu F, Ji A, Zhang Z, Li J and Li P: miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4. Exp Ther Med 21: 622, 2021.
APA
Wu, F., Ji, A., Zhang, Z., Li, J., & Li, P. (2021). miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4. Experimental and Therapeutic Medicine, 21, 622. https://doi.org/10.3892/etm.2021.10054
MLA
Wu, F., Ji, A., Zhang, Z., Li, J., Li, P."miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4". Experimental and Therapeutic Medicine 21.6 (2021): 622.
Chicago
Wu, F., Ji, A., Zhang, Z., Li, J., Li, P."miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4". Experimental and Therapeutic Medicine 21, no. 6 (2021): 622. https://doi.org/10.3892/etm.2021.10054
Copy and paste a formatted citation
x
Spandidos Publications style
Wu F, Ji A, Zhang Z, Li J and Li P: miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4. Exp Ther Med 21: 622, 2021.
APA
Wu, F., Ji, A., Zhang, Z., Li, J., & Li, P. (2021). miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4. Experimental and Therapeutic Medicine, 21, 622. https://doi.org/10.3892/etm.2021.10054
MLA
Wu, F., Ji, A., Zhang, Z., Li, J., Li, P."miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4". Experimental and Therapeutic Medicine 21.6 (2021): 622.
Chicago
Wu, F., Ji, A., Zhang, Z., Li, J., Li, P."miR‑491‑5p inhibits the proliferation and migration of A549 cells by FOXP4". Experimental and Therapeutic Medicine 21, no. 6 (2021): 622. https://doi.org/10.3892/etm.2021.10054
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