miR‑1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS‑induced cell model of sepsis

Ling,Ping; Tang,Rong; Wang,Huazhu; Deng,Xiuqin; Chen,Jianli

doi:10.3892/etm.2021.10062

miR‑1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS‑induced cell model of sepsis

Authors:
- Ping Ling
- Rong Tang
- Huazhu Wang
- Xiuqin Deng
- Jianli Chen
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Affiliations: Pediatric Intensive Care Unit, Guiyang Maternal and Child Health Care Hospital, Guiyang, Guizhou 550003, P.R. China
Published online on: April 15, 2021 https://doi.org/10.3892/etm.2021.10062
Article Number: 630
Copyright: © Ling et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) have been reported to be potential clinical biomarkers for sepsis. miR‑1184 is a multifunctional microRNA that exerts roles in the development of various diseases. However, the role of miR‑1184 in children with sepsis remain unknown. In the present study, THP‑1 cells were stimulated with 1 µg/ml lipopolysaccharide (LPS) for 24 h to establish an in vitro sepsis model. Reverse transcription‑quantitative PCR was used to evaluate the expression of miR‑1184 in clinical specimens, and of IL‑6, TNF‑α, IL‑1β, miR‑1184 and TNF receptor type 1‑associated DEATH domain protein (TRADD) in cells with and without LPS treatment. Cell apoptosis was assessed using flow cytometry. Binding between miR‑1184 and TRADD was predicted using bioinformatics software, and a luciferase reporter assay was performed to verify the interaction between miR‑1184 and TRADD in LPS‑induced THP‑1 cells. In addition, western blot analysis was performed to detect TRADD and proteins associated with the NF‑κB pathway. The results showed that miR‑1184 was downregulated in the blood of children with sepsis and LPS‑induced THP‑1 cells. Overexpression of miR‑1184 alleviated the LPS‑induced production of inflammatory cytokines and cell apoptosis. Moreover, TRADD was verified to be a direct target of miR‑1184. Upregulation of TRADD reversed the effects of miR‑1184 on the LPS‑induced inflammatory response and apoptosis of THP‑1 cells. Furthermore, the NF‑κB pathway was shown to be associated with the regulatory role of miR‑1184 in sepsis. The present study provides evidence that miR‑1184 exerts inhibitory effects on inflammatory responses and apoptosis in sepsis by targeting TRADD, which suggests that miR‑1184 may be a novel potential target for the therapy of children with sepsis.

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