Open Access

miR‑221‑3p and miR‑222‑3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer

  • Authors:
    • Ting Ye
    • Lili Zhong
    • Xuemei Ye
    • Jie Liu
    • Linfa Li
    • Heqing Yi
  • View Affiliations

  • Published online on: April 19, 2021     https://doi.org/10.3892/etm.2021.10084
  • Article Number: 652
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The expression levels of microRNA (miR)‑221‑3p and miR‑222‑3p in thyroid cancer have been found to be upregulated compared with those in normal tissues. The present study aimed to determine the effects and potential underlying mechanisms of miR‑221‑3p and miR‑222‑3p on the regulation of radioactive iodine (131I) uptake and radiosensitivity of thyroid cancer cells. The potential regulatory target genes of miR‑221‑3p and miR‑222‑3p were predicted by bioinformatics analysis, and reverse transcription‑quantitative polymerase chain reaction was used to verify miR‑221‑3p, miR‑222‑3p and target gene expression levels in thyroid cancer tissues and cell lines. Overexpression of miR‑221‑3p or miR‑222‑3p in cell models was performed using lentivirus infection. Knockdown of miR‑221‑3p and miR‑222‑3p in cells was achieved using oligonucleotide inhibitor transfection. Western blotting was used to analyze the expression levels of target proteins. In addition, the effects of miR‑221‑3p and miR‑222‑3p on the radiosensitivity of thyroid cancer cells were verified using a colony formation assay. The results of the present study revealed that the expression levels of miR‑221‑3p and miR‑222‑3p were significantly upregulated, while the expression levels of suppressor of cytokine signaling 3 (SOCS3) were downregulated in thyroid cancer tissues. Furthermore, miR‑221‑3p and miR‑222‑3p overexpression downregulated the expression levels of SOCS3, E‑cadherin and solute carrier family 5 member 5 (NIS), and upregulated the expression levels of phosphorylated STAT3 and vimentin. Following the overexpression of miR‑221‑3p or miR‑222‑3p in the FTC133 and TPC1 cell lines, their radiosensitivity was suppressed. In conclusion, the findings of the present study suggested that miR‑221‑3p and miR‑222‑3p may downregulate the expression levels of NIS and promote radioresistance. The potential mechanism was hypothesized to be associated with the miR‑221‑3p and miR‑222‑3p targeting of the SOCS3 gene, which may subsequently activate the STAT3 signaling pathway.

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June-2021
Volume 21 Issue 6

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Spandidos Publications style
Ye T, Zhong L, Ye X, Liu J, Li L and Yi H: miR‑221‑3p and miR‑222‑3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer. Exp Ther Med 21: 652, 2021
APA
Ye, T., Zhong, L., Ye, X., Liu, J., Li, L., & Yi, H. (2021). miR‑221‑3p and miR‑222‑3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer. Experimental and Therapeutic Medicine, 21, 652. https://doi.org/10.3892/etm.2021.10084
MLA
Ye, T., Zhong, L., Ye, X., Liu, J., Li, L., Yi, H."miR‑221‑3p and miR‑222‑3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer". Experimental and Therapeutic Medicine 21.6 (2021): 652.
Chicago
Ye, T., Zhong, L., Ye, X., Liu, J., Li, L., Yi, H."miR‑221‑3p and miR‑222‑3p regulate the SOCS3/STAT3 signaling pathway to downregulate the expression of NIS and reduce radiosensitivity in thyroid cancer". Experimental and Therapeutic Medicine 21, no. 6 (2021): 652. https://doi.org/10.3892/etm.2021.10084