Open Access

Apoptosis in HUVECs induced by microRNA‑616‑3p via X‑linked inhibitor of apoptosis protein targeting

  • Authors:
    • Hua Chen
    • Xi Liu
    • Yun Wu
    • Xiayin Wu
    • Xiaoli Wen
    • Yanan Lu
    • Xingsheng Zhao
  • View Affiliations

  • Published online on: April 21, 2021     https://doi.org/10.3892/etm.2021.10093
  • Article Number: 661
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atherosclerosis causes stroke and coronary heart disease and is associated with a high mortality rate worldwide. However, the pathogenesis of atherosclerosis remains unclear. Endothelial cell apoptosis is one of the early changes observed in atherosclerosis. Previous studies have found that microRNA (miR)‑616‑3p may be involved in the development of atherosclerosis, but the specific mechanism is not clear. The present study aimed to investigate whether miR‑616‑3p is involved in endothelial cell apoptosis and its underlying mechanism. The present study demonstrated that compared with normal HUVECs, HUVECs treated with oxidized low‑density lipoprotein expressed higher miR‑616‑3p and lower X‑linked inhibitor of apoptosis protein (XIAP) levels. In the present study, HUVECs were transfected with miR‑616‑3p mimic and Cell Counting Kit‑8 (CCK‑8), flow cytometry and TUNEL staining assays demonstrated that compared with miR‑616‑3p mimic control, the miR‑616‑3p mimic promoted HUVEC apoptosis. In addition, using StarBase 3.0 for bioinformatics analysis it was predicted that miR‑616‑3p may bind to the 3'untranslated region (UTR) of XIAP mRNA. The present study performed the CCK‑8, flow cytometry, TUNEL staining and dual‑luciferase reporter assays and demonstrated that miR‑616‑3p binds to the 3'UTR of the XIAP mRNA and inhibits its expression and that this further promotes apoptosis in HUVECs. In addition, western blotting demonstrated that compared with miR‑616‑3p mimic control, the miR‑616‑3p mimic increases the level of cleaved caspase‑3 in HUVECs. In summary, the present study demonstrated that miR‑616‑3p can directly inhibit the expression of XIAP mRNA by targeting its 3'UTR which promoted apoptosis in HUVECs. miR‑616‑3p and XIAP may be used as therapeutic targets of atherosclerosis in the future.

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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen H, Liu X, Wu Y, Wu X, Wen X, Lu Y and Zhao X: Apoptosis in HUVECs induced by microRNA‑616‑3p via X‑linked inhibitor of apoptosis protein targeting. Exp Ther Med 21: 661, 2021
APA
Chen, H., Liu, X., Wu, Y., Wu, X., Wen, X., Lu, Y., & Zhao, X. (2021). Apoptosis in HUVECs induced by microRNA‑616‑3p via X‑linked inhibitor of apoptosis protein targeting. Experimental and Therapeutic Medicine, 21, 661. https://doi.org/10.3892/etm.2021.10093
MLA
Chen, H., Liu, X., Wu, Y., Wu, X., Wen, X., Lu, Y., Zhao, X."Apoptosis in HUVECs induced by microRNA‑616‑3p via X‑linked inhibitor of apoptosis protein targeting". Experimental and Therapeutic Medicine 21.6 (2021): 661.
Chicago
Chen, H., Liu, X., Wu, Y., Wu, X., Wen, X., Lu, Y., Zhao, X."Apoptosis in HUVECs induced by microRNA‑616‑3p via X‑linked inhibitor of apoptosis protein targeting". Experimental and Therapeutic Medicine 21, no. 6 (2021): 661. https://doi.org/10.3892/etm.2021.10093