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Article

A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction

  • Authors:
    • Yewei Yin
    • Tu Hu
    • Aiqun Xiang
    • Yanyan Fu
    • Yang Zhao
    • Xiaoying Wu
    • Xiaoying Wu
    • Dan Wen
  • View Affiliations / Copyright

    Affiliations: Eye Center of Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pathology, College of Basic Medicine, Central South University, Changsha, Hunan 410008, P.R. China
  • Article Number: 681
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    Published online on: April 25, 2021
       https://doi.org/10.3892/etm.2021.10113
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Abstract

The aim of the present study was to investigate the histological and morphological characteristics of corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction (SMILE) surgery by light and electron microscopy. A total of 20 human corneal stromal lenticules extracted during SMILE surgery were sent for microscopic examination immediately after surgery. Of these, six were observed under a light microscope and 14 were observed under an electron microscope. The smoothness of the front and rear surface of the lenticules observed under an electron microscope was rated on a scale of 0 to 4 according to unified evaluation criteria and the scores were statistically compared. Under the light microscope, the edge of the cross section of the corneal stromal lenticules was deeply stained and certain burrs and broken collagen fibers were observed. The swollen corneal stromal fibers were distributed irregularly, with a few bubbles of different sizes. Under the electron microscope, the surface of the lenticules ablated using a femtosecond laser was not perfectly smooth and the front surface was smoother than the rear surface. The side edge of the lenticules ablated using a femtosecond laser was not as regular as the edge cut using microscissors. Necrosis and dissolution of collagen fibers were observed near the surface of the lenticules. In conclusion, the surface quality of corneal stromal lenticules ablated using a femtosecond laser was not optimal when observed under a microscope. Increased attention should be paid to the histology and morphology of the corneal surface following laser ablation.
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Copy and paste a formatted citation
Spandidos Publications style
Yin Y, Hu T, Xiang A, Fu Y, Zhao Y, Wu X, Wu X and Wen D: A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction. Exp Ther Med 22: 681, 2021.
APA
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X. ... Wen, D. (2021). A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction. Experimental and Therapeutic Medicine, 22, 681. https://doi.org/10.3892/etm.2021.10113
MLA
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X., Wu, X., Wen, D."A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction". Experimental and Therapeutic Medicine 22.1 (2021): 681.
Chicago
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X., Wu, X., Wen, D."A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction". Experimental and Therapeutic Medicine 22, no. 1 (2021): 681. https://doi.org/10.3892/etm.2021.10113
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Hu T, Xiang A, Fu Y, Zhao Y, Wu X, Wu X and Wen D: A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction. Exp Ther Med 22: 681, 2021.
APA
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X. ... Wen, D. (2021). A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction. Experimental and Therapeutic Medicine, 22, 681. https://doi.org/10.3892/etm.2021.10113
MLA
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X., Wu, X., Wen, D."A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction". Experimental and Therapeutic Medicine 22.1 (2021): 681.
Chicago
Yin, Y., Hu, T., Xiang, A., Fu, Y., Zhao, Y., Wu, X., Wu, X., Wen, D."A microscopic study of the corneal stromal lenticules extracted during femtosecond laser‑assisted small incision lenticule extraction". Experimental and Therapeutic Medicine 22, no. 1 (2021): 681. https://doi.org/10.3892/etm.2021.10113
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