miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A

Pan,Yaozhen; Zhan,Lei; Chen,Ling; Chen,Liwen; Sun,Chengyi

doi:10.3892/etm.2021.10115

miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A

Authors:
- Yaozhen Pan
- Lei Zhan
- Ling Chen
- Liwen Chen
- Chengyi Sun
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Affiliations: Department of Biliary-Hepatic Surgery, The Affiliated Tumor Hospital of Guizhou Medical University, Guiyang, Guizhou 550000, P.R China, Department of Biliary-Hepatic Surgery, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550000, P.R China, Department of Biliary-Hepatic Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, P.R China
Published online on: April 25, 2021 https://doi.org/10.3892/etm.2021.10115
Article Number: 683

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Abstract

Liver cancer (LC) is the leading cause for tumor‑related death worldwide, and microRNAs (miRs) have been demonstrated to regulate the progression of LC. In the current study, the function of miR‑660 in LC cells was investigated, and the results indicated that miR‑660 was highly expressed in LC tissues and cells. This increased expression promoted LC cell proliferation and increased the percentage of S phase cells, while miR‑660 knockdown inhibited cell proliferation and increased the percentage of G₀/G₁ phase cells. A Ser/Thr phosphatase protein phosphatase 2 regulatory subunit βα (PPP2R2A) was indicated as the target of miR‑660, and miR‑660 could inhibit PPP2R2A levels. The luciferase reporter assay suggested that miR‑660 directly bound to the 3'‑untranslated region of PPP2R2A. Additionally, it was revealed that miR‑660 inhibited p21 expression and promoted cyclin D1 expression, confirming that miR‑660 regulated LC cell proliferation by regulating cell cycle progression. The double knockdown of miR‑660 and PPP2R2A promoted LC cell proliferation, suggesting that miR‑660 promoted LC proliferation by targeting PPP2R2A.

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