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Article

miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A

  • Authors:
    • Yaozhen Pan
    • Lei Zhan
    • Ling Chen
    • Liwen Chen
    • Chengyi Sun
  • View Affiliations / Copyright

    Affiliations: Department of Biliary-Hepatic Surgery, The Affiliated Tumor Hospital of Guizhou Medical University, Guiyang, Guizhou 550000, P.R China, Department of Biliary-Hepatic Surgery, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550000, P.R China, Department of Biliary-Hepatic Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, P.R China
  • Article Number: 683
    |
    Published online on: April 25, 2021
       https://doi.org/10.3892/etm.2021.10115
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Abstract

Liver cancer (LC) is the leading cause for tumor‑related death worldwide, and microRNAs (miRs) have been demonstrated to regulate the progression of LC. In the current study, the function of miR‑660 in LC cells was investigated, and the results indicated that miR‑660 was highly expressed in LC tissues and cells. This increased expression promoted LC cell proliferation and increased the percentage of S phase cells, while miR‑660 knockdown inhibited cell proliferation and increased the percentage of G0/G1 phase cells. A Ser/Thr phosphatase protein phosphatase 2 regulatory subunit βα (PPP2R2A) was indicated as the target of miR‑660, and miR‑660 could inhibit PPP2R2A levels. The luciferase reporter assay suggested that miR‑660 directly bound to the 3'‑untranslated region of PPP2R2A. Additionally, it was revealed that miR‑660 inhibited p21 expression and promoted cyclin D1 expression, confirming that miR‑660 regulated LC cell proliferation by regulating cell cycle progression. The double knockdown of miR‑660 and PPP2R2A promoted LC cell proliferation, suggesting that miR‑660 promoted LC proliferation by targeting PPP2R2A.
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1 

Dong Q, Li C, Che X, Qu J, Fan Y, Li X, Li Y, Wang Q, Liu Y, Yang X and Qu X: MicroRNA-891b is an independent prognostic factor of pancreatic cancer by targeting Cbl-b to suppress the growth of pancreatic cancer cells. Oncotarget. 7:82338–82353. 2016.PubMed/NCBI View Article : Google Scholar

2 

Ørom UA, Nielsen FC and Lund AH: MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation. Mol Cell. 30:460–471. 2008.PubMed/NCBI View Article : Google Scholar

3 

Liu M, Roth A, Yu M, Morris R, Bersani F, Rivera MN, Lu J, Shioda T, Vasudevan S, Ramaswamy S, et al: The IGF2 intronic miR-483 selectively enhances transcription from IGF2 fetal promoters and enhances tumorigenesis. Genes Dev. 27:2543–2548. 2013.PubMed/NCBI View Article : Google Scholar

4 

Lin Q, Ma L, Liu Z, Yang Z, Wang J, Liu J and Jiang G: Targeting microRNAs: A new action mechanism of natural compounds. Oncotarget. 8:15961–15970. 2017.PubMed/NCBI View Article : Google Scholar

5 

Xu L, Dai W, Li J, He L, Wang F, Xia Y, Chen K, Li S, Liu T, Lu J, et al: Methylation-regulated miR-124-1 suppresses tumorigenesis in hepatocellular carcinoma by targeting CASC3. Oncotarget. 7:26027–26041. 2016.PubMed/NCBI View Article : Google Scholar

6 

Fang Y, Xue JL, Shen Q, Chen J and Tian L: MicroRNA-7 inhibits tumor growth and metastasis by targeting the phosphoinositide 3-kinase/Akt pathway in hepatocellular carcinoma. Hepatology. 55:1852–1862. 2012.PubMed/NCBI View Article : Google Scholar

7 

Liu CC, Lin SP, Hsu HS, Yang SH, Lin CH, Yang MH, Hung MC and Hung SC: Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells. Nat Commun. 7(11798)2016.PubMed/NCBI View Article : Google Scholar

8 

Cristóbal I, Manso R, Rincón R, Caramés C, Senin C, Borrero A, Martínez-Useros J, Rodriguez M, Zazo S, Aguilera O, et al: PP2A inhibition is a common event in colorectal cancer and its restoration using FTY720 shows promising therapeutic potential. Mol Cancer Ther. 13:938–947. 2014.PubMed/NCBI View Article : Google Scholar

9 

Wang Q, Li J, Wu W, Shen R, Jiang H, Qian Y, Tang Y, Bai T, Wu S, Wei L, et al: Smad4-dependent suppressor pituitary homeobox 2 promotes PPP2R2A-mediated inhibition of Akt pathway in pancreatic cancer. Oncotarget. 7:11208–11222. 2016.PubMed/NCBI View Article : Google Scholar

10 

Li X, Zhang J and Ma D: PPP2R2A binds and dephosphorylates GFPT2 in breast cancer cells. Sheng Wu Gong Cheng Xue Bao. 34:956–963. 2018.PubMed/NCBI View Article : Google Scholar : (In Chinese).

11 

Qin Q, Wang X, Yan N, Song RH, Cai TT, Zhang W, Guan LJ, Muhali FS and Zhang JA: Aberrant expression of miRNA and mRNAs in lesioned tissues of Graves' disease. Cell Physiol Biochem. 35:1934–1942. 2015.PubMed/NCBI View Article : Google Scholar

12 

Dmitriev P, Stankevicins L, Ansseau E, Petrov A, Barat A, Dessen P, Robert T, Turki A, Lazar V, Labourer E, et al: Defective regulation of microRNA target genes in myoblasts from facioscapulohumeral dystrophy patients. J Biol Chem. 288:34989–35002. 2013.PubMed/NCBI View Article : Google Scholar

13 

Satoh J, Kino Y and Niida S: MicroRNA-seq data analysis pipeline to identify blood biomarkers for Alzheimer's disease from public data. Biomarker insights. 10:21–31. 2015.PubMed/NCBI View Article : Google Scholar

14 

Fortunato O, Boeri M, Moro M, Verri C, Mensah M, Conte D, Caleca L, Roz L, Pastorino U and Sozzi G: Mir-660 is downregulated in lung cancer patients and its replacement inhibits lung tumorigenesis by targeting MDM2-p53 interaction. Cell Death Dis. 5(e1564)2014.PubMed/NCBI View Article : Google Scholar

15 

Cribbs AP, Kennedy A, Gregory B and Brennan FM: Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells. BMC Biotechnol. 13(98)2013.PubMed/NCBI View Article : Google Scholar

16 

Lin H, Dai T, Xiong H, Zhao X, Chen X, Yu C, Li J, Wang X and Song L: Unregulated miR-96 induces cell proliferation in human breast cancer by downregulating transcriptional factor FOXO3a. PLoS One. 5(e15797)2010.PubMed/NCBI View Article : Google Scholar

17 

Alvarez-Fernández M, Halim VA, Aprelia M, Laoukili J, Mohammed S and Medema RH: Protein phosphatase 2A (B55α) prevents premature activation of forkhead transcription factor FoxM1 by antagonizing cyclin A/cyclin-dependent kinase-mediated phosphorylation. J Biol Chem. 286:33029–33036. 2011.PubMed/NCBI View Article : Google Scholar

18 

Forte D, Salvestrini V, Corradi G, Rossi L, Catani L, Lemoli RM, Cavo M and Curti A: The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling. Oncotarget. 8:2261–2274. 2017.PubMed/NCBI View Article : Google Scholar

19 

Pestell RG: New roles of cyclin D1. Am J Pathol. 183:3–9. 2013.PubMed/NCBI View Article : Google Scholar

20 

Xiao F, Zhang W, Chen L, Chen F, Xie H, Xing C, Yu X, Ding S, Chen K, Guo H, et al: MicroRNA-503 inhibits the G1/S transition by downregulating cyclin D3 and E2F3 in hepatocellular carcinoma. J Transl Med. 11(195)2013.PubMed/NCBI View Article : Google Scholar

21 

Simerzin A, Zorde-Khvalevsky E, Rivkin M, Adar R, Zucman-Rossi J, Couchy G, Roskams T, Govaere O, Oren M, Giladi H and Galun E: The liver-specific microRNA-122*, the complementary strand of microRNA-122, acts as a tumor suppressor by modulating the p53/mouse double minute 2 homolog circuitry. Hepatology. 64:1623–1636. 2016.PubMed/NCBI View Article : Google Scholar

22 

Fei L and Xu H: Role of MCM2-7 protein phosphorylation in human cancer cells. Cell Biosci. 8(43)2018.PubMed/NCBI View Article : Google Scholar

23 

Zhao W, Cao L, Zeng S, Qin H and Men T: Upregulation of miR-556-5p promoted prostate cancer cell proliferation by suppressing PPP2R2A expression. Biomed Pharmacother. 75:142–147. 2015.PubMed/NCBI View Article : Google Scholar

24 

Shen S, Yue H, Li Y, Qin J, Li K, Liu Y and Wang J: Upregulation of miR-136 in human non-small cell lung cancer cells promotes Erk1/2 activation by targeting PPP2R2A. Tumour Biol. 35:631–640. 2014.PubMed/NCBI View Article : Google Scholar

25 

Liang WL, Cao J, Xu B, Yang P, Shen F, Sun Z, Li WL, Wang Q and Liu F: miR-892a regulated PPP2R2A expression and promoted cell proliferation of human colorectal cancer cells. Biomed Pharmacother. 72:119–124. 2015.PubMed/NCBI View Article : Google Scholar

26 

Zeng LP, Hu ZM, Li K and Xia K: miR-222 attenuates cisplatin-induced cell death by targeting the PPP2R2A/Akt/mTOR Axis in bladder cancer cells. J Cell Mol Med. 20:559–567. 2016.PubMed/NCBI View Article : Google Scholar

27 

Wong QW, Ching AK, Chan AW, Choy KW, To KF, Lai PB and Wong N: MiR-222 overexpression confers cell migratory advantages in hepatocellular carcinoma through enhancing AKT signaling. Clin Cancer Res. 16:867–875. 2010.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Pan Y, Zhan L, Chen L, Chen L and Sun C: miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A. Exp Ther Med 22: 683, 2021.
APA
Pan, Y., Zhan, L., Chen, L., Chen, L., & Sun, C. (2021). miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A. Experimental and Therapeutic Medicine, 22, 683. https://doi.org/10.3892/etm.2021.10115
MLA
Pan, Y., Zhan, L., Chen, L., Chen, L., Sun, C."miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A". Experimental and Therapeutic Medicine 22.1 (2021): 683.
Chicago
Pan, Y., Zhan, L., Chen, L., Chen, L., Sun, C."miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A". Experimental and Therapeutic Medicine 22, no. 1 (2021): 683. https://doi.org/10.3892/etm.2021.10115
Copy and paste a formatted citation
x
Spandidos Publications style
Pan Y, Zhan L, Chen L, Chen L and Sun C: miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A. Exp Ther Med 22: 683, 2021.
APA
Pan, Y., Zhan, L., Chen, L., Chen, L., & Sun, C. (2021). miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A. Experimental and Therapeutic Medicine, 22, 683. https://doi.org/10.3892/etm.2021.10115
MLA
Pan, Y., Zhan, L., Chen, L., Chen, L., Sun, C."miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A". Experimental and Therapeutic Medicine 22.1 (2021): 683.
Chicago
Pan, Y., Zhan, L., Chen, L., Chen, L., Sun, C."miR‑660 promotes liver cancer cell proliferation by targeting PPP2R2A". Experimental and Therapeutic Medicine 22, no. 1 (2021): 683. https://doi.org/10.3892/etm.2021.10115
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