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Article Open Access

Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model

  • Authors:
    • Weiwei Bao
    • Zhigang Xiao
    • Zhiqing Wang
    • Donglin Liu
    • Ping Tan
    • Mingfang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, 900 Hospital of The Joint Logistics Team, Fujian Medical University, Fuzhou, Fujian 350025, P.R. China, Department of Cadre Health Care, 900 Hospital of The Joint Logistics Team, Fujian Medical University, Fuzhou, Fujian 350025, P.R. China, Graduate College of Fujian Medical University, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 739
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    Published online on: May 9, 2021
       https://doi.org/10.3892/etm.2021.10171
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Abstract

Long non‑coding RNAs (lncRNAs) have been identified as a class of regulatory RNAs that participate in both physiological and pathological conditions, including acute kidney injury. However, the roles of lncRNA dysregulation in the pathogenesis of contrast‑induced acute kidney injury (CI‑AKI) are largely unknown. In the present study, the expression profiles of lncRNAs in kidney tissue were compared between rats with CI‑AKI and controls using high‑throughput RNA sequencing. In total, 910 differentially expressed (DE) lncRNAs (DElncRNAs), including 415 downregulated and 495 upregulated lncRNAs, were identified at 12 h after intra‑arterial iodinated contrast medium injection (fold change ≥2; P<0.05). Eight DElncRNAs were further selected and validated using reverse transcription‑quantitative polymerase chain reaction. A previous study defined microRNA (miRNA) and mRNA expression changes in the same CI‑AKI model. In the present study, a lncRNA‑mRNA co‑expression network comprising 349 DElncRNAs and 202 DEmRNAs was constructed. The function of these DElncRNAs was mainly associated with oxidative stress and inflammation. Additionally, lncRNA‑associated competing endogenous RNA (ceRNA) analysis revealed a network comprising 40 DElncRNA nodes, 5 DEmiRNA nodes and 59 DEmRNA nodes. Among which, the carnosine dipeptidase 1‑specific and the transmembrane protein 184B‑specific networks were likely to be associated with CI‑AKI. The results of the present study revealed the expression profile and potential roles of lncRNAs in CI‑AKI, and provide a framework for further mechanistic studies.
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Bao W, Xiao Z, Wang Z, Liu D, Tan P and Huang M: Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model. Exp Ther Med 22: 739, 2021.
APA
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., & Huang, M. (2021). Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model. Experimental and Therapeutic Medicine, 22, 739. https://doi.org/10.3892/etm.2021.10171
MLA
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., Huang, M."Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model". Experimental and Therapeutic Medicine 22.1 (2021): 739.
Chicago
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., Huang, M."Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model". Experimental and Therapeutic Medicine 22, no. 1 (2021): 739. https://doi.org/10.3892/etm.2021.10171
Copy and paste a formatted citation
x
Spandidos Publications style
Bao W, Xiao Z, Wang Z, Liu D, Tan P and Huang M: Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model. Exp Ther Med 22: 739, 2021.
APA
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., & Huang, M. (2021). Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model. Experimental and Therapeutic Medicine, 22, 739. https://doi.org/10.3892/etm.2021.10171
MLA
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., Huang, M."Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model". Experimental and Therapeutic Medicine 22.1 (2021): 739.
Chicago
Bao, W., Xiao, Z., Wang, Z., Liu, D., Tan, P., Huang, M."Comprehensive analysis of the long non‑coding RNA expression profile and functional roles in a contrast‑induced acute kidney injury rat model". Experimental and Therapeutic Medicine 22, no. 1 (2021): 739. https://doi.org/10.3892/etm.2021.10171
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