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Effects of Salvia miltiorrhiza extract on lung adenocarcinoma

  • Authors:
    • Huixiang Tian
    • Yueqin Li
    • Jie Mei
    • Lei Cao
    • Jiye Yin
    • Zhaoqian Liu
    • Juan Chen
    • Xiangping Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 794
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    Published online on: May 25, 2021
       https://doi.org/10.3892/etm.2021.10226
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Abstract

Lung adenocarcinoma is the most common subtype of non‑small cell lung carcinoma. Tanshinone I is an important fat‑soluble component in the extract of Salvia miltiorrhiza that has been reported to inhibit lung adenocarcinoma cell proliferation. However, no studies have clearly demonstrated changes in lung adenocarcinoma gene expression and signaling pathway enrichment following Tanshinone I treatment. And it remains unclear whether salvianolate has an effect on lung adenocarcinoma. The present study downloaded the GSE9315 dataset from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) and the underlying signaling pathways involved after Tanshinone I administration in the lung adenocarcinoma cell line CL1‑5. The results revealed that there were 28 and 102 DEGs in the low dosage group (0.01 and 0.10 µg/ml Tanshinone I) and medium dosage groups (1 and 10 µg/ml Tanshinone I), respectively. In the low dosage group, DEGs were mainly enriched in ‘positive regulation of T‑helper cell differentiation’ and ‘protein complex’. In the medium dosage group, 102 DEGs were enriched in ‘MAPK cascade’ and ‘extracellular exosome’. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated enrichment of both groups in the PI3K‑Akt signaling pathway. Furthermore, there were nine overlapping DEGs [ADP ribosylation factor‑interacting protein 2, chemokine (C‑X‑C motif) ligand 6, SH2 domain‑containing adaptor protein B, Src homology 2 domain‑containing transforming protein1, collagen type VI α1 chain, elastin, integrin subunit α, endoplasmic reticulum mannosyl‑oligosaccharide 1,2‑α‑mannosidase and sterile α motif domain‑containing 9 like] between the two groups, which serve to be potential targets for the treatment of lung adenocarcinoma. The present study also investigated the possible effects of salvianolate on lung adenocarcinoma in vivo using nude mouse xenograft models injected with the A549 cell line. The data revealed that salvianolate not only suppressed lung adenocarcinoma tumor growth of in nude mice, but also downregulated the expression levels of ATP7A and ATP7B, which are important proteins in the tumorigenesis and chemotherapy of lung adenocarcinoma. The present study provided evidence for the potential use of Salvia miltiorrhiza extract for treating lung adenocarcinomas in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Tian H, Li Y, Mei J, Cao L, Yin J, Liu Z, Chen J and Li X: Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma. Exp Ther Med 22: 794, 2021.
APA
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z. ... Li, X. (2021). Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma. Experimental and Therapeutic Medicine, 22, 794. https://doi.org/10.3892/etm.2021.10226
MLA
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z., Chen, J., Li, X."Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma". Experimental and Therapeutic Medicine 22.2 (2021): 794.
Chicago
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z., Chen, J., Li, X."Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma". Experimental and Therapeutic Medicine 22, no. 2 (2021): 794. https://doi.org/10.3892/etm.2021.10226
Copy and paste a formatted citation
x
Spandidos Publications style
Tian H, Li Y, Mei J, Cao L, Yin J, Liu Z, Chen J and Li X: Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma. Exp Ther Med 22: 794, 2021.
APA
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z. ... Li, X. (2021). Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma. Experimental and Therapeutic Medicine, 22, 794. https://doi.org/10.3892/etm.2021.10226
MLA
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z., Chen, J., Li, X."Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma". Experimental and Therapeutic Medicine 22.2 (2021): 794.
Chicago
Tian, H., Li, Y., Mei, J., Cao, L., Yin, J., Liu, Z., Chen, J., Li, X."Effects of <em>Salvia miltiorrhiza</em> extract on lung adenocarcinoma". Experimental and Therapeutic Medicine 22, no. 2 (2021): 794. https://doi.org/10.3892/etm.2021.10226
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