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The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR

  • Authors:
    • Donghui Zhou
    • Xiaohua Zhu
    • Xuan Wu
    • Jingjing Zheng
    • Laizhen Tou
    • Yong Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of General Surgery, Lishui Municipal Central Hospital, Lishui, Zhejiang 323000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 798
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    Published online on: May 25, 2021
       https://doi.org/10.3892/etm.2021.10230
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Abstract

Gastric cancer (GC) poses a serious threat to human health worldwide. Serine/arginine rich splicing factor 1 (SRSF1) has been reported to serve regulatory roles during the tumorigenesis of GC. In addition, the macrophage stimulating 1 receptor (MST1R) signaling pathway was found to participate in the progression of GC. However, the association between MST1R and SRSF1 in the tumorigenesis of GC remains unclear. The expression levels of MST1R and the recepteur d'origine nantais (RON) Δ160 splicing variant were analyzed in cells using western blotting and immunofluorescence staining. Co‑immunoprecipitation assays were used to investigate the interaction between SRSF1 and MST1R. A Cell Counting Kit‑8 assay was performed to analyze cell viability. Flow cytometry and Transwell assays were used to determine cell apoptosis and invasiveness levels. The potential interaction between SFSR1 and long non‑coding RNAs (lncRNAs) was investigated with an online bioinformatics tool. The findings of the present study revealed that the expression levels of MST1R and RON Δ160 were significantly upregulated in GC Kato III cells. SRSF1 was found to be regulated by the lncRNA FOXF1 adjacent non‑coding developmental regulatory RNA (FENDRR). The knockdown of SRSF1 or FENDRR downregulated the expression levels of MST1R in Kato III cells. In addition, the expression levels of RON Δ160 were markedly downregulated in Kato III cells following the knockdown of FENDRR. Meanwhile, SRSF1 directly bound to MST1R, while this phenomenon was partially reversed by FENDRR short interfering RNA. FENDRR could interact with SRSF1 in Kato III cells and the knockdown of FENDRR also induced the apoptosis of GC cells. In conclusion, the findings of the present study suggested that the lncRNA FENDRR may function as an oncogene during the progression of GC by regulating alternative splicing of MST1R and SRSF1 expression levels. lncRNA FENDRR may serve as a potential marker for the diagnosis or target for the treatment of GC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou D, Zhu X, Wu X, Zheng J, Tou L and Zhou Y: The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR. Exp Ther Med 22: 798, 2021.
APA
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., & Zhou, Y. (2021). The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR. Experimental and Therapeutic Medicine, 22, 798. https://doi.org/10.3892/etm.2021.10230
MLA
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., Zhou, Y."The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR". Experimental and Therapeutic Medicine 22.2 (2021): 798.
Chicago
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., Zhou, Y."The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR". Experimental and Therapeutic Medicine 22, no. 2 (2021): 798. https://doi.org/10.3892/etm.2021.10230
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou D, Zhu X, Wu X, Zheng J, Tou L and Zhou Y: The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR. Exp Ther Med 22: 798, 2021.
APA
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., & Zhou, Y. (2021). The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR. Experimental and Therapeutic Medicine, 22, 798. https://doi.org/10.3892/etm.2021.10230
MLA
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., Zhou, Y."The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR". Experimental and Therapeutic Medicine 22.2 (2021): 798.
Chicago
Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., Zhou, Y."The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR". Experimental and Therapeutic Medicine 22, no. 2 (2021): 798. https://doi.org/10.3892/etm.2021.10230
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