Open Access

Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes

  • Authors:
    • Yong Xie
    • Qian Li
    • Hai-Feng Zhang
    • Tu-Cheng Huang
    • Ying Yang
    • Yong-Qing Lin
    • Jing-Ting Mai
    • Zhu-Zhi Wen
    • Wo-Liang Yuan
    • Jing-Feng Wang
    • Yang-Xin Chen
  • View Affiliations

  • Published online on: May 28, 2021     https://doi.org/10.3892/etm.2021.10247
  • Article Number: 815
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous previous studies have found that C‑reactive protein (CRP) is associated with cardiac arrhythmia and cardiac remodeling. However, the underlying mechanisms of this association remain unclear. Sodium‑calcium exchanger 1 (NCX1) serves an important role in the regulation of intracellular calcium concentration, which is closely related with cardiac arrhythmia and cardiac remodeling. The present study aimed to evaluate the effects of CRP on NCX1 and intracellular calcium concentration in cardiomyocytes. Primary neonatal mouse ventricular cardiomyocytes were cultured and treated with varying concentrations of CRP (0, 5, 10, 20 and 40 µg/ml). The cardiomyocytes were also treated with NF‑κB‑specific inhibitor PTDC and a specific inhibitor of the reverse NCX1 KB‑R7943 before their intracellular calcium concentrations were measured. mRNA and protein expression levels of NCX1 were detected by reverse transcription‑quantitative PCR and western blotting, respectively and intracellular calcium concentration was evaluated by flow cytometry. CRP treatment significantly increased mRNA and protein expression levels of NCX1 in myocytes (P=0.024), as well as intracellular calcium concentration (P=0.01). These results were significantly attenuated by the NF‑κB‑specific inhibitor PDTC and a specific inhibitor of the reverse NCX1, KB‑R7943. CRP significantly upregulated NCX1 expression and increased intracellular calcium concentration in cardiomyocytes via the NF‑κB pathway, suggesting that CRP may serve a pro‑arrhythmia role via direct influence on the calcium homeostasis of cardiomyocytes.
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August-2021
Volume 22 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xie Y, Li Q, Zhang H, Huang T, Yang Y, Lin Y, Mai J, Wen Z, Yuan W, Wang J, Wang J, et al: Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Exp Ther Med 22: 815, 2021
APA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y. ... Chen, Y. (2021). Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Experimental and Therapeutic Medicine, 22, 815. https://doi.org/10.3892/etm.2021.10247
MLA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22.2 (2021): 815.
Chicago
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 815. https://doi.org/10.3892/etm.2021.10247