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Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes

  • Authors:
    • Yong Xie
    • Qian Li
    • Hai-Feng Zhang
    • Tu-Cheng Huang
    • Ying Yang
    • Yong-Qing Lin
    • Jing-Ting Mai
    • Zhu-Zhi Wen
    • Wo-Liang Yuan
    • Jing-Feng Wang
    • Yang-Xin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Sun Yat‑sen Memorial Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Dermatology, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 515110, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 815
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    Published online on: May 28, 2021
       https://doi.org/10.3892/etm.2021.10247
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Abstract

Numerous previous studies have found that C‑reactive protein (CRP) is associated with cardiac arrhythmia and cardiac remodeling. However, the underlying mechanisms of this association remain unclear. Sodium‑calcium exchanger 1 (NCX1) serves an important role in the regulation of intracellular calcium concentration, which is closely related with cardiac arrhythmia and cardiac remodeling. The present study aimed to evaluate the effects of CRP on NCX1 and intracellular calcium concentration in cardiomyocytes. Primary neonatal mouse ventricular cardiomyocytes were cultured and treated with varying concentrations of CRP (0, 5, 10, 20 and 40 µg/ml). The cardiomyocytes were also treated with NF‑κB‑specific inhibitor PTDC and a specific inhibitor of the reverse NCX1 KB‑R7943 before their intracellular calcium concentrations were measured. mRNA and protein expression levels of NCX1 were detected by reverse transcription‑quantitative PCR and western blotting, respectively and intracellular calcium concentration was evaluated by flow cytometry. CRP treatment significantly increased mRNA and protein expression levels of NCX1 in myocytes (P=0.024), as well as intracellular calcium concentration (P=0.01). These results were significantly attenuated by the NF‑κB‑specific inhibitor PDTC and a specific inhibitor of the reverse NCX1, KB‑R7943. CRP significantly upregulated NCX1 expression and increased intracellular calcium concentration in cardiomyocytes via the NF‑κB pathway, suggesting that CRP may serve a pro‑arrhythmia role via direct influence on the calcium homeostasis of cardiomyocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Xie Y, Li Q, Zhang H, Huang T, Yang Y, Lin Y, Mai J, Wen Z, Yuan W, Wang J, Wang J, et al: Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Exp Ther Med 22: 815, 2021.
APA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y. ... Chen, Y. (2021). Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Experimental and Therapeutic Medicine, 22, 815. https://doi.org/10.3892/etm.2021.10247
MLA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22.2 (2021): 815.
Chicago
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 815. https://doi.org/10.3892/etm.2021.10247
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Y, Li Q, Zhang H, Huang T, Yang Y, Lin Y, Mai J, Wen Z, Yuan W, Wang J, Wang J, et al: Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Exp Ther Med 22: 815, 2021.
APA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y. ... Chen, Y. (2021). Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes. Experimental and Therapeutic Medicine, 22, 815. https://doi.org/10.3892/etm.2021.10247
MLA
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22.2 (2021): 815.
Chicago
Xie, Y., Li, Q., Zhang, H., Huang, T., Yang, Y., Lin, Y., Mai, J., Wen, Z., Yuan, W., Wang, J., Chen, Y."Effect of C reactive protein on the sodium‑calcium exchanger 1 in cardiomyocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 815. https://doi.org/10.3892/etm.2021.10247
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