Open Access

Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes

  • Authors:
    • Chi-Cheng Lu
    • Jai-Sing Yang
    • Yu-Jen Chiu
    • Fuu-Jen Tsai
    • Yuan-Man Hsu
    • Mei-Chin Yin
    • Yu-Ning Juan
    • Tsung-Jung Ho
    • Hao-Ping Chen
  • View Affiliations

  • Published online on: June 2, 2021     https://doi.org/10.3892/etm.2021.10254
  • Article Number: 822
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dracorhodin can be isolated from the exudates of the fruit of Daemonorops draco. Previous studies suggested that dracorhodin perchlorate can promote fibroblast proliferation and enhance angiogenesis during wound healing. In the present study, the potential bioactivity of dracorhodin perchlorate in human HaCaT keratinocytes, were investigated in vitro, with specific focus on HaCaT wound healing. The results of in vitro scratch assay demonstrated the progressive closure of the wound after treatment with dracorhodin perchlorate in a time‑dependent manner. An MTT assay and propidium iodide exclusion detected using flow cytometry were used to detect cell viability of HaCaT cells. Potential signaling pathways underlying the effects mediated by dracorhodin perchlorate in HaCaT cells were clarified by western blot analysis and kinase activity assays. Dracorhodin perchlorate significantly increased the protein expression levels of β‑catenin and activation of AKT, ERK and p38 in HaCaT cells. In addition, dracorhodin perchlorate did not induce HaCaT cell proliferation but promoted cell migration. Other mechanisms may yet be involved in the dracorhodin perchlorate‑induced wound healing process of human keratinocytes. In summary, dracorhodin perchlorate may serve to be a potential molecularly‑targeted phytochemical that can improve skin wound healing.
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August-2021
Volume 22 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lu C, Yang J, Chiu Y, Tsai F, Hsu Y, Yin M, Juan Y, Ho T and Chen H: Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Exp Ther Med 22: 822, 2021
APA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M. ... Chen, H. (2021). Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Experimental and Therapeutic Medicine, 22, 822. https://doi.org/10.3892/etm.2021.10254
MLA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22.2 (2021): 822.
Chicago
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 822. https://doi.org/10.3892/etm.2021.10254