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Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes

  • Authors:
    • Chi-Cheng Lu
    • Jai-Sing Yang
    • Yu-Jen Chiu
    • Fuu-Jen Tsai
    • Yuan-Man Hsu
    • Mei-Chin Yin
    • Yu-Ning Juan
    • Tsung-Jung Ho
    • Hao-Ping Chen
  • View Affiliations / Copyright

    Affiliations: Department of Sport Performance, National Taiwan University of Sport, Taichung 40404, Taiwan, R.O.C., Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan, R.O.C., Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veteran General Hospital, Taipei 11217, Taiwan, R.O.C., Human Genetics Center, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan, R.O.C., Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan, R.O.C.
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 822
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    Published online on: June 2, 2021
       https://doi.org/10.3892/etm.2021.10254
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Abstract

Dracorhodin can be isolated from the exudates of the fruit of Daemonorops draco. Previous studies suggested that dracorhodin perchlorate can promote fibroblast proliferation and enhance angiogenesis during wound healing. In the present study, the potential bioactivity of dracorhodin perchlorate in human HaCaT keratinocytes, were investigated in vitro, with specific focus on HaCaT wound healing. The results of in vitro scratch assay demonstrated the progressive closure of the wound after treatment with dracorhodin perchlorate in a time‑dependent manner. An MTT assay and propidium iodide exclusion detected using flow cytometry were used to detect cell viability of HaCaT cells. Potential signaling pathways underlying the effects mediated by dracorhodin perchlorate in HaCaT cells were clarified by western blot analysis and kinase activity assays. Dracorhodin perchlorate significantly increased the protein expression levels of β‑catenin and activation of AKT, ERK and p38 in HaCaT cells. In addition, dracorhodin perchlorate did not induce HaCaT cell proliferation but promoted cell migration. Other mechanisms may yet be involved in the dracorhodin perchlorate‑induced wound healing process of human keratinocytes. In summary, dracorhodin perchlorate may serve to be a potential molecularly‑targeted phytochemical that can improve skin wound healing.
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Copy and paste a formatted citation
Spandidos Publications style
Lu C, Yang J, Chiu Y, Tsai F, Hsu Y, Yin M, Juan Y, Ho T and Chen H: Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Exp Ther Med 22: 822, 2021.
APA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M. ... Chen, H. (2021). Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Experimental and Therapeutic Medicine, 22, 822. https://doi.org/10.3892/etm.2021.10254
MLA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22.2 (2021): 822.
Chicago
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 822. https://doi.org/10.3892/etm.2021.10254
Copy and paste a formatted citation
x
Spandidos Publications style
Lu C, Yang J, Chiu Y, Tsai F, Hsu Y, Yin M, Juan Y, Ho T and Chen H: Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Exp Ther Med 22: 822, 2021.
APA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M. ... Chen, H. (2021). Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes. Experimental and Therapeutic Medicine, 22, 822. https://doi.org/10.3892/etm.2021.10254
MLA
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22.2 (2021): 822.
Chicago
Lu, C., Yang, J., Chiu, Y., Tsai, F., Hsu, Y., Yin, M., Juan, Y., Ho, T., Chen, H."Dracorhodin perchlorate enhances wound healing via β‑catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes". Experimental and Therapeutic Medicine 22, no. 2 (2021): 822. https://doi.org/10.3892/etm.2021.10254
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