Macrophages induce the expression of lncRNA ATB via the secretion of TGF‑β to relieve ischemia‑reperfusion injury in cardiomyocytes

Lin,Hong; Xu,Wen-Sheng; Liu,Xiong-Wei; Wang,Zhi; Yan,Jiao; Zhang,Tao

doi:10.3892/etm.2021.10342

Macrophages induce the expression of lncRNA ATB via the secretion of TGF‑β to relieve ischemia‑reperfusion injury in cardiomyocytes

Authors:
- Hong Lin
- Wen-Sheng Xu
- Xiong-Wei Liu
- Zhi Wang
- Jiao Yan
- Tao Zhang
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Affiliations: Department of Functional Diagnosis, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014040, P.R. China, Department of Immunology, Basic and Forensic Medicine of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014040, P.R. China
Published online on: June 29, 2021 https://doi.org/10.3892/etm.2021.10342
Article Number: 910
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cardiac ischemia‑reperfusion can cause severe damage to cardiomyocytes. Previous studies have revealed that TGF‑β can alleviate ischemia‑reperfusion injury in cardiomyocytes by inducing the expression of long non‑coding RNA (lncRNA) activated by TGF‑β (ATB). However, M2 macrophages can secrete a large amount of TGF‑β. However, whether M2 macrophages alleviate the ischemia‑reperfusion‑induced injury of cardiomyocytes by secreting TGF‑β is unclear. In the present study, macrophages and cardiomyocytes were cultured under oxygen‑glucose deprivation/reoxygenation (OGD/R) conditions to simulate ischemia‑reperfusion injury. M2‑type macrophage markers (IL‑10, Arginase‑1 and IL‑13) were validated using reverse transcription‑quantitative PCR and western blotting. Subsequently, the culture medium of M2‑type macrophages was collected for the treatment of cardiomyocytes, which were cultured under OGD/R conditions. The levels of inflammatory factors and oxidase enzymes were detected with ELISA. The apoptotic rates of cardiomyocytes were detected by flow cytometry. The expression of cell apoptosis‑related proteins and the phosphorylation levels of NF‑κB were analyzed by western blotting. The expression levels of specific inflammatory cytokines and the levels of malondialdehyde and lactate dehydrogenase were suppressed in cardiomyocytes following treatment with culture medium derived from M2‑type macrophages, which were cultured under OGD/R conditions. Furthermore, OGD/R‑induced apoptosis of cardiomyocytes was also relieved following treatment of the cells with macrophage medium. It was found that M2‑type macrophages could secrete TGF‑β and that the culture medium of M2‑type macrophages could activate the expression of lncRNA ATB in cardiomyocytes. TGF‑β secreted by M2 macrophages relieved the inflammatory response, oxidative stress and apoptosis of cardiomyocytes by inducing the expression of lncRNA ATB.

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